ID   HPNC_SINFN              Reviewed;         292 AA.
AC   P55351;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   25-MAY-2022, entry version 122.
DE   RecName: Full=Hydroxysqualene synthase {ECO:0000250|UniProtKB:Q6N3F1};
DE            Short=HSQ synthase {ECO:0000250|UniProtKB:Q6N3F1};
DE            Short=HSQase {ECO:0000250|UniProtKB:Q6N3F1};
DE            EC=4.2.3.156 {ECO:0000250|UniProtKB:Q6N3F1};
GN   OrderedLocusNames=NGR_a00430; ORFNames=y4aD;
OS   Sinorhizobium fredii (strain NBRC 101917 / NGR234).
OG   Plasmid sym pNGR234a.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=394;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 101917 / NGR234;
RX   PubMed=9163424; DOI=10.1038/387394a0;
RA   Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A.,
RA   Perret X.;
RT   "Molecular basis of symbiosis between Rhizobium and legumes.";
RL   Nature 387:394-401(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 101917 / NGR234;
RX   PubMed=19376903; DOI=10.1128/aem.00515-09;
RA   Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A.,
RA   Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A.,
RA   Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A.,
RA   Broughton W.J., Perret X., Strittmatter A.W., Streit W.R.;
RT   "Rhizobium sp. strain NGR234 possesses a remarkable number of secretion
RT   systems.";
RL   Appl. Environ. Microbiol. 75:4035-4045(2009).
CC   -!- FUNCTION: Involved in the biosynthesis of the hopanoid precursor
CC       squalene (SQ) from farnesyl diphosphate (FPP). Catalyzes the second
CC       step, the conversion of presqualene diphosphate (PSPP) to
CC       hydroxysqualene (HSQ). {ECO:0000250|UniProtKB:Q6N3F1}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + presqualene diphosphate = diphosphate + hydroxysqualene;
CC         Xref=Rhea:RHEA:47984, ChEBI:CHEBI:15377, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:57310, ChEBI:CHEBI:88123; EC=4.2.3.156;
CC         Evidence={ECO:0000250|UniProtKB:Q6N3F1};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; hopanoid biosynthesis.
CC       {ECO:0000250|UniProtKB:Q6N3F1}.
CC   -!- SIMILARITY: Belongs to the phytoene/squalene synthase family. HpnC
CC       subfamily. {ECO:0000305}.
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DR   EMBL; U00090; AAB91602.1; -; Genomic_DNA.
DR   RefSeq; NP_443764.1; NC_000914.2.
DR   RefSeq; WP_010875085.1; NC_000914.2.
DR   AlphaFoldDB; P55351; -.
DR   SMR; P55351; -.
DR   STRING; 394.NGR_a00430; -.
DR   EnsemblBacteria; AAB91602; AAB91602; NGR_a00430.
DR   KEGG; rhi:NGR_a00430; -.
DR   PATRIC; fig|394.7.peg.40; -.
DR   eggNOG; COG1562; Bacteria.
DR   HOGENOM; CLU_037269_0_1_5; -.
DR   OMA; DYCRYSA; -.
DR   OrthoDB; 1449625at2; -.
DR   UniPathway; UPA00337; -.
DR   Proteomes; UP000001054; Plasmid sym pNGR234a.
DR   GO; GO:0004311; F:farnesyltranstransferase activity; IEA:InterPro.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   GO; GO:0009058; P:biosynthetic process; IEA:InterPro.
DR   CDD; cd00683; Trans_IPPS_HH; 1.
DR   Gene3D; 1.10.600.10; -; 1.
DR   InterPro; IPR017827; HSQ_synthase_HpnC.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR002060; Squ/phyt_synthse.
DR   InterPro; IPR044843; Trans_IPPS_bact-type.
DR   InterPro; IPR033904; Trans_IPPS_HH.
DR   PANTHER; PTHR31480:SF17; PTHR31480:SF17; 1.
DR   Pfam; PF00494; SQS_PSY; 1.
DR   SFLD; SFLDG01212; Phytoene_synthase_like; 1.
DR   SUPFAM; SSF48576; SSF48576; 1.
DR   TIGRFAMs; TIGR03464; HpnC; 1.
PE   3: Inferred from homology;
KW   Lyase; Plasmid; Reference proteome.
FT   CHAIN           1..292
FT                   /note="Hydroxysqualene synthase"
FT                   /id="PRO_0000200798"
SQ   SEQUENCE   292 AA;  32540 MW;  1055E5E7F3614379 CRC64;
     MTSGSGPRSD AMHREKNFPV ASWIIHPRHR ALILAFYRFV RMADDIADHA TLAPDEKLLY
     LDLLEAELLG KGETRAEAVH LRTALDRRGM PPRHALDLLT AFRMDVTKQR YENWDEVIDY
     CRYSAMPVGR FVLDVHGESA ATWPASDVLC AGLQVCNHLQ DCGKDFLNLN RVYIPRDALS
     ASGASIEELG AAKSSLQMLQ CLRSLAAKAE VLLNGGGALV SQVKDFRLRF EVSVILSFAD
     KIVSMLKMRD PLRERVHLSP LELLLHGVGA MANEAARRAF GRGTRFKTTV DV