ID   HPND_RHOPA              Reviewed;         279 AA.
AC   Q6N3F2;
DT   27-SEP-2017, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Presqualene diphosphate synthase {ECO:0000303|PubMed:26258173};
DE            Short=PSPP synthase {ECO:0000303|PubMed:26258173};
DE            Short=PSPPase {ECO:0000303|PubMed:26258173};
DE            EC=2.5.1.103 {ECO:0000269|PubMed:26258173};
GN   Name=hpnD {ECO:0000303|PubMed:26258173};
GN   OrderedLocusNames=RPA3742 {ECO:0000312|EMBL:CAE29183.1};
OS   Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bradyrhizobiaceae; Rhodopseudomonas.
OX   NCBI_TaxID=258594;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-98 / CGA009;
RX   PubMed=14704707; DOI=10.1038/nbt923;
RA   Larimer F.W., Chain P., Hauser L., Lamerdin J.E., Malfatti S., Do L.,
RA   Land M.L., Pelletier D.A., Beatty J.T., Lang A.S., Tabita F.R.,
RA   Gibson J.L., Hanson T.E., Bobst C., Torres y Torres J.L., Peres C.,
RA   Harrison F.H., Gibson J., Harwood C.S.;
RT   "Complete genome sequence of the metabolically versatile photosynthetic
RT   bacterium Rhodopseudomonas palustris.";
RL   Nat. Biotechnol. 22:55-61(2004).
RN   [2]
RP   FUNCTION, CATALYTIC ACTIVITY, AND PATHWAY.
RC   STRAIN=BisB5;
RX   PubMed=26258173; DOI=10.1021/acscentsci.5b00115;
RA   Pan J.J., Solbiati J.O., Ramamoorthy G., Hillerich B.S., Seidel R.D.,
RA   Cronan J.E., Almo S.C., Poulter C.D.;
RT   "Biosynthesis of squalene from farnesyl diphosphate in bacteria: three
RT   steps catalyzed by three enzymes.";
RL   ACS Cent. Sci. 1:77-82(2015).
CC   -!- FUNCTION: Involved in the biosynthesis of the hopanoid precursor
CC       squalene (SQ) from farnesyl diphosphate (FPP). Catalyzes the first
CC       step, the formation of presqualene diphosphate (PSPP) from two
CC       molecules of FPP. {ECO:0000269|PubMed:26258173}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 (2E,6E)-farnesyl diphosphate = diphosphate + presqualene
CC         diphosphate; Xref=Rhea:RHEA:22672, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:57310, ChEBI:CHEBI:175763; EC=2.5.1.103;
CC         Evidence={ECO:0000269|PubMed:26258173};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; hopanoid biosynthesis.
CC       {ECO:0000269|PubMed:26258173}.
CC   -!- SIMILARITY: Belongs to the phytoene/squalene synthase family. HpnD
CC       subfamily. {ECO:0000305}.
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DR   EMBL; BX572605; CAE29183.1; -; Genomic_DNA.
DR   RefSeq; WP_011159280.1; NC_005296.1.
DR   AlphaFoldDB; Q6N3F2; -.
DR   SMR; Q6N3F2; -.
DR   STRING; 258594.RPA3742; -.
DR   PRIDE; Q6N3F2; -.
DR   DNASU; 2692931; -.
DR   EnsemblBacteria; CAE29183; CAE29183; RPA3742.
DR   GeneID; 66894848; -.
DR   KEGG; rpa:RPA3742; -.
DR   eggNOG; COG1562; Bacteria.
DR   HOGENOM; CLU_037269_1_1_5; -.
DR   OMA; VKAPRIM; -.
DR   PhylomeDB; Q6N3F2; -.
DR   BioCyc; RPAL258594:TX73_RS19100-MON; -.
DR   UniPathway; UPA00337; -.
DR   Proteomes; UP000001426; Chromosome.
DR   GO; GO:0004311; F:farnesyltranstransferase activity; IEA:InterPro.
DR   GO; GO:0016767; F:geranylgeranyl-diphosphate geranylgeranyltransferase activity; IEA:InterPro.
DR   GO; GO:0016117; P:carotenoid biosynthetic process; IEA:InterPro.
DR   CDD; cd00683; Trans_IPPS_HH; 1.
DR   Gene3D; 1.10.600.10; -; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR017828; SQ_synth_HpnD-like.
DR   InterPro; IPR002060; Squ/phyt_synthse.
DR   InterPro; IPR019845; Squalene/phytoene_synthase_CS.
DR   InterPro; IPR044843; Trans_IPPS_bact-type.
DR   InterPro; IPR033904; Trans_IPPS_HH.
DR   Pfam; PF00494; SQS_PSY; 1.
DR   SFLD; SFLDG01212; Phytoene_synthase_like; 1.
DR   SUPFAM; SSF48576; SSF48576; 1.
DR   TIGRFAMs; TIGR03465; HpnD; 1.
DR   PROSITE; PS01044; SQUALEN_PHYTOEN_SYN_1; 1.
DR   PROSITE; PS01045; SQUALEN_PHYTOEN_SYN_2; 1.
PE   1: Evidence at protein level;
KW   Reference proteome; Transferase.
FT   CHAIN           1..279
FT                   /note="Presqualene diphosphate synthase"
FT                   /id="PRO_0000441693"
SQ   SEQUENCE   279 AA;  30906 MW;  64D8036612604A23 CRC64;
     MTVHATPEPA AHQGVALGSS FYAAMRILPR PQREAMFQVY SFCRFVDDIA DSDRPREQRV
     AELQQWRDDI AALYRGAPPP RLADYQESLR TFGLKREDFE AIIDGMEMDV DADIRAPDEA
     TLDLYCDRVA SAVGRLSVRI FGLPEADGIE LSHHLGRALQ LTNILRDIDE DAGIGRLYLP
     SELLHKVGIT ATDPRVVAAD SALPSVCAPL VERALAHFAA ADKVMNRNPR RVVKAPRIMG
     KYYYSILQLL IARGFAAPRA PVKLGKASKI AILLQYAIV