ID   HPND_SINFN              Reviewed;         279 AA.
AC   P55350;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   25-MAY-2022, entry version 119.
DE   RecName: Full=Presqualene diphosphate synthase {ECO:0000250|UniProtKB:Q6N3F2};
DE            Short=PSPP synthase {ECO:0000250|UniProtKB:Q6N3F2};
DE            Short=PSPPase {ECO:0000250|UniProtKB:Q6N3F2};
DE            EC=2.5.1.103 {ECO:0000250|UniProtKB:Q6N3F2};
GN   OrderedLocusNames=NGR_a00440; ORFNames=y4aC;
OS   Sinorhizobium fredii (strain NBRC 101917 / NGR234).
OG   Plasmid sym pNGR234a.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=394;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 101917 / NGR234;
RX   PubMed=9163424; DOI=10.1038/387394a0;
RA   Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A.,
RA   Perret X.;
RT   "Molecular basis of symbiosis between Rhizobium and legumes.";
RL   Nature 387:394-401(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 101917 / NGR234;
RX   PubMed=19376903; DOI=10.1128/aem.00515-09;
RA   Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A.,
RA   Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A.,
RA   Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A.,
RA   Broughton W.J., Perret X., Strittmatter A.W., Streit W.R.;
RT   "Rhizobium sp. strain NGR234 possesses a remarkable number of secretion
RT   systems.";
RL   Appl. Environ. Microbiol. 75:4035-4045(2009).
CC   -!- FUNCTION: Involved in the biosynthesis of the hopanoid precursor
CC       squalene (SQ) from farnesyl diphosphate (FPP). Catalyzes the first
CC       step, the formation of presqualene diphosphate (PSPP) from two
CC       molecules of FPP. {ECO:0000250|UniProtKB:Q6N3F2}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 (2E,6E)-farnesyl diphosphate = diphosphate + presqualene
CC         diphosphate; Xref=Rhea:RHEA:22672, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:57310, ChEBI:CHEBI:175763; EC=2.5.1.103;
CC         Evidence={ECO:0000250|UniProtKB:Q6N3F2};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; hopanoid biosynthesis.
CC       {ECO:0000250|UniProtKB:Q6N3F2}.
CC   -!- SIMILARITY: Belongs to the phytoene/squalene synthase family. HpnD
CC       subfamily. {ECO:0000305}.
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DR   EMBL; U00090; AAB91601.1; -; Genomic_DNA.
DR   RefSeq; NP_443763.1; NC_000914.2.
DR   RefSeq; WP_010875086.1; NC_000914.2.
DR   AlphaFoldDB; P55350; -.
DR   SMR; P55350; -.
DR   STRING; 394.NGR_a00440; -.
DR   EnsemblBacteria; AAB91601; AAB91601; NGR_a00440.
DR   KEGG; rhi:NGR_a00440; -.
DR   PATRIC; fig|394.7.peg.41; -.
DR   eggNOG; COG1562; Bacteria.
DR   HOGENOM; CLU_037269_1_1_5; -.
DR   OMA; VKAPRIM; -.
DR   OrthoDB; 1449625at2; -.
DR   UniPathway; UPA00337; -.
DR   Proteomes; UP000001054; Plasmid sym pNGR234a.
DR   GO; GO:0004311; F:farnesyltranstransferase activity; IEA:InterPro.
DR   GO; GO:0016767; F:geranylgeranyl-diphosphate geranylgeranyltransferase activity; IEA:InterPro.
DR   GO; GO:0016117; P:carotenoid biosynthetic process; IEA:InterPro.
DR   CDD; cd00683; Trans_IPPS_HH; 1.
DR   Gene3D; 1.10.600.10; -; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR017828; SQ_synth_HpnD-like.
DR   InterPro; IPR002060; Squ/phyt_synthse.
DR   InterPro; IPR019845; Squalene/phytoene_synthase_CS.
DR   InterPro; IPR044843; Trans_IPPS_bact-type.
DR   InterPro; IPR033904; Trans_IPPS_HH.
DR   Pfam; PF00494; SQS_PSY; 1.
DR   SFLD; SFLDG01212; Phytoene_synthase_like; 1.
DR   SFLD; SFLDG01018; Squalene/Phytoene_Synthase_Lik; 1.
DR   SUPFAM; SSF48576; SSF48576; 1.
DR   TIGRFAMs; TIGR03465; HpnD; 1.
DR   PROSITE; PS01044; SQUALEN_PHYTOEN_SYN_1; 1.
DR   PROSITE; PS01045; SQUALEN_PHYTOEN_SYN_2; 1.
PE   3: Inferred from homology;
KW   Plasmid; Reference proteome; Transferase.
FT   CHAIN           1..279
FT                   /note="Presqualene diphosphate synthase"
FT                   /id="PRO_0000067442"
SQ   SEQUENCE   279 AA;  31354 MW;  DD236CB8F72BAFC5 CRC64;
     MRAEAAAHAN HRSTALGSSF YLGMRTLPPV QREAIFQIYS FCRQVDDIAD SDEPREHRLA
     ALQQWRDHID ALYQCVPPPR LKDYLASVTT FGLKREDFLA VVDGMEMDVL QDIRAPKMAT
     LDLYCDRVAS AVGRMSVRVF GLSEEDGIAL AHHLGRALQL TNILRDIDED AGLGRLYIPR
     ESLDHAGITS SDPHKVIADK ALPKACAPLA QRAMMHFAES DEIMNCNPRR IVRAPTIMSK
     CYRAILDLLL IRGFAAPREP VRVTNLTKRA ILFRYALML