ID   HPPA2_RHILT             Reviewed;         215 AA.
AC   Q93AS1;
DT   25-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   25-MAY-2022, entry version 63.
DE   RecName: Full=Pyrophosphate-energized proton pump 2;
DE            EC=7.1.3.1;
DE   AltName: Full=Membrane-bound proton-translocating pyrophosphatase 2;
DE   AltName: Full=Pyrophosphate-energized inorganic pyrophosphatase 2;
DE            Short=H(+)-PPase 2;
DE   Flags: Fragment;
GN   Name=hppA2;
OS   Rhizobium leguminosarum bv. trifolii.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX   NCBI_TaxID=386;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 14480 / 3D1k22a;
RA   Jumas-Bilak E., Michaux-Charachon S., Teyssier C.;
RT   "High prevalence of the H+-proton-pumping inorganic pyrophosphatase gene in
RT   alpha proteobacteria and evidence of lateral transfer in its phylogeny.";
RL   Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Proton pump that utilizes the energy of pyrophosphate
CC       hydrolysis as the driving force for proton movement across the
CC       membrane. Generates a proton motive force (By similarity).
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=diphosphate + H(+)(in) + H2O = 2 H(+)(out) + 2 phosphate;
CC         Xref=Rhea:RHEA:13973, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:43474; EC=7.1.3.1;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the H(+)-translocating pyrophosphatase (TC
CC       3.A.10) family. {ECO:0000305}.
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DR   EMBL; AF417516; AAL14975.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q93AS1; -.
DR   SMR; Q93AS1; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0004427; F:inorganic diphosphatase activity; IEA:InterPro.
DR   GO; GO:0009678; F:pyrophosphate hydrolysis-driven proton transmembrane transporter activity; IEA:UniProtKB-EC.
DR   InterPro; IPR004131; PPase-energised_H-pump.
DR   PANTHER; PTHR31998; PTHR31998; 1.
DR   Pfam; PF03030; H_PPase; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Hydrogen ion transport; Ion transport;
KW   Magnesium; Membrane; Translocase; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           <1..>215
FT                   /note="Pyrophosphate-energized proton pump 2"
FT                   /id="PRO_0000217026"
FT   TRANSMEM        16..36
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        51..71
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        86..106
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        136..156
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        164..184
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   NON_TER         1
FT   NON_TER         215
SQ   SEQUENCE   215 AA;  21711 MW;  9B8D950E65B48318 CRC64;
     AAIFFAGTPI LESAMVYPLA ICGACILTSI AGTFFVKLGT NNSIMGALYK GLIATGVFSV
     AGLAVATYAT VGWGTIGTVA GMEITGTNLF FCGLVGLVVT ALIVVITEYY TGTNKRPVNS
     IAQASVTGHG TNVIQGLAVS LESTALPAIV IVGGIIGTYQ LGGLFGTGIA VTAMLGLAGM
     IVALDAFGPV TDNAGGIAEM AGLDPDVRKR PTRWM