ID   HPPA_ALLVI              Reviewed;         197 AA.
AC   Q8VNU8;
DT   25-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   25-MAY-2022, entry version 67.
DE   RecName: Full=K(+)-insensitive pyrophosphate-energized proton pump;
DE            EC=7.1.3.1;
DE   AltName: Full=Membrane-bound proton-translocating pyrophosphatase;
DE   AltName: Full=Pyrophosphate-energized inorganic pyrophosphatase;
DE            Short=H(+)-PPase;
DE   Flags: Fragment;
GN   Name=hppA; Synonyms=vppA;
OS   Allochromatium vinosum (Chromatium vinosum).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Chromatiaceae;
OC   Allochromatium.
OX   NCBI_TaxID=1049;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Perez-Castineira J.R., Losada M., Serrano A.;
RT   "Evidence for a wide distribution of proton-translocating pyrophosphatases
RT   among photosynthetic organisms.";
RL   Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Proton pump that utilizes the energy of pyrophosphate
CC       hydrolysis as the driving force for proton movement across the
CC       membrane. Generates a proton motive force (By similarity).
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=diphosphate + H(+)(in) + H2O = 2 H(+)(out) + 2 phosphate;
CC         Xref=Rhea:RHEA:13973, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:43474; EC=7.1.3.1;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the H(+)-translocating pyrophosphatase (TC
CC       3.A.10) family. K(+)-insensitive subfamily. {ECO:0000305}.
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DR   EMBL; AJ251726; CAC80903.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q8VNU8; -.
DR   SMR; Q8VNU8; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0004427; F:inorganic diphosphatase activity; IEA:InterPro.
DR   GO; GO:0009678; F:pyrophosphate hydrolysis-driven proton transmembrane transporter activity; IEA:UniProtKB-EC.
DR   InterPro; IPR004131; PPase-energised_H-pump.
DR   PANTHER; PTHR31998; PTHR31998; 1.
DR   Pfam; PF03030; H_PPase; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Hydrogen ion transport; Ion transport;
KW   Magnesium; Membrane; Translocase; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           <1..>197
FT                   /note="K(+)-insensitive pyrophosphate-energized proton
FT                   pump"
FT                   /id="PRO_0000217016"
FT   TRANSMEM        30..50
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        70..90
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        139..159
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        168..188
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   SITE            29
FT                   /note="Determinant of potassium independence"
FT                   /evidence="ECO:0000250"
FT   NON_TER         1
FT   NON_TER         197
SQ   SEQUENCE   197 AA;  20155 MW;  A22803C29E83EA58 CRC64;
     AGGIAEMSGL PEDVRKTTDA LDAVGNTTKA VTKGYAIGSA GLAALVLFAD YTHNLEVAGK
     MQVFSLSDPA VIIGLFIGGL VPYLFGAMAM EAVGRAAGSV VIEVRRQFRE IKGIMEGTAK
     PDYSRAVDLL TKAAIKEMVV PSLLPILVPV AVAIGMNILM GDGAGIRALG GMLIGTIVTG
     LFVAISMCTG GGAWDNA