ID   HPPA_ANAMA              Reviewed;         141 AA.
AC   Q8VRZ1;
DT   25-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   02-JUN-2021, entry version 58.
DE   RecName: Full=Pyrophosphate-energized proton pump;
DE            EC=7.1.3.1;
DE   AltName: Full=Membrane-bound proton-translocating pyrophosphatase;
DE   AltName: Full=Pyrophosphate-energized inorganic pyrophosphatase;
DE            Short=H(+)-PPase;
DE   Flags: Fragment;
GN   Name=hppA;
OS   Anaplasma marginale.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Anaplasmataceae; Anaplasma.
OX   NCBI_TaxID=770;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Jumas-Bilak E., Michaux-Charachon S., Teyssier C.;
RT   "High prevalence of the H+ proton-pumping inorganic pyrophosphatase gene in
RT   alpha proteobacteria and evidence of lateral transfer in its phylogeny.";
RL   Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Proton pump that utilizes the energy of pyrophosphate
CC       hydrolysis as the driving force for proton movement across the
CC       membrane. Generates a proton motive force (By similarity).
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=diphosphate + H(+)(in) + H2O = 2 H(+)(out) + 2 phosphate;
CC         Xref=Rhea:RHEA:13973, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:43474; EC=7.1.3.1;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the H(+)-translocating pyrophosphatase (TC
CC       3.A.10) family. {ECO:0000305}.
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DR   EMBL; AF417515; AAL69331.1; -; Genomic_DNA.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0004427; F:inorganic diphosphatase activity; IEA:InterPro.
DR   GO; GO:0009678; F:pyrophosphate hydrolysis-driven proton transmembrane transporter activity; IEA:UniProtKB-EC.
DR   InterPro; IPR004131; PPase-energised_H-pump.
DR   PANTHER; PTHR31998; PTHR31998; 1.
DR   Pfam; PF03030; H_PPase; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Hydrogen ion transport; Ion transport;
KW   Magnesium; Membrane; Translocase; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           <1..>141
FT                   /note="Pyrophosphate-energized proton pump"
FT                   /id="PRO_0000217009"
FT   TRANSMEM        11..31
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        46..66
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        121..141
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   NON_TER         1
FT   NON_TER         141
SQ   SEQUENCE   141 AA;  14012 MW;  6B684177277A6E1C CRC64;
     NGSIMGALYK GLIATGLLSI VGLGVANTLT VGWGEIGTVA GKSITGTNLF VCGLIGLIVT
     GLIVVITEYY TGTNKRPVNS XAQASVTGHG TNVIQGLAVS LESTALPAIV IVGGIIXTYQ
     LAGLFGTAIA VTAMLGIAGM I