HPPA_BRUA4
ID HPPA_BRUA4 Reviewed; 718 AA.
AC Q8VRZ2; A6X1Y3;
DT 25-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 13-NOV-2007, sequence version 2.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=K(+)-insensitive pyrophosphate-energized proton pump {ECO:0000255|HAMAP-Rule:MF_01129};
DE EC=7.1.3.1 {ECO:0000255|HAMAP-Rule:MF_01129};
DE AltName: Full=Membrane-bound proton-translocating pyrophosphatase {ECO:0000255|HAMAP-Rule:MF_01129};
DE AltName: Full=Pyrophosphate-energized inorganic pyrophosphatase {ECO:0000255|HAMAP-Rule:MF_01129};
DE Short=H(+)-PPase {ECO:0000255|HAMAP-Rule:MF_01129};
GN Name=hppA {ECO:0000255|HAMAP-Rule:MF_01129}; OrderedLocusNames=Oant_2524;
OS Brucella anthropi (strain ATCC 49188 / DSM 6882 / CCUG 24695 / JCM 21032 /
OS LMG 3331 / NBRC 15819 / NCTC 12168 / Alc 37) (Ochrobactrum anthropi).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX NCBI_TaxID=439375;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 49188 / DSM 6882 / CCUG 24695 / JCM 21032 / LMG 3331 / NBRC
RC 15819 / NCTC 12168 / Alc 37;
RX PubMed=21685287; DOI=10.1128/jb.05335-11;
RA Chain P.S., Lang D.M., Comerci D.J., Malfatti S.A., Vergez L.M., Shin M.,
RA Ugalde R.A., Garcia E., Tolmasky M.E.;
RT "Genome of Ochrobactrum anthropi ATCC 49188 T, a versatile opportunistic
RT pathogen and symbiont of several eukaryotic hosts.";
RL J. Bacteriol. 193:4274-4275(2011).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 242-455.
RA Jumas-Bilak E., Michaux-Charachon S., Teyssier C.;
RT "High prevalence of the H+ proton-pumping inorganic pyrophosphatase gene in
RT alpha proteobacteria and evidence of lateral transfer in its phylogeny.";
RL Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Proton pump that utilizes the energy of pyrophosphate
CC hydrolysis as the driving force for proton movement across the
CC membrane. Generates a proton motive force. {ECO:0000255|HAMAP-
CC Rule:MF_01129}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=diphosphate + H(+)(in) + H2O = 2 H(+)(out) + 2 phosphate;
CC Xref=Rhea:RHEA:13973, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:43474; EC=7.1.3.1;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01129};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01129};
CC -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01129}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01129}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_01129}.
CC -!- SIMILARITY: Belongs to the H(+)-translocating pyrophosphatase (TC
CC 3.A.10) family. K(+)-insensitive subfamily. {ECO:0000255|HAMAP-
CC Rule:MF_01129}.
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DR EMBL; CP000758; ABS15237.1; -; Genomic_DNA.
DR EMBL; AF417514; AAL69330.1; -; Genomic_DNA.
DR RefSeq; WP_012092338.1; NC_009667.1.
DR AlphaFoldDB; Q8VRZ2; -.
DR SMR; Q8VRZ2; -.
DR EnsemblBacteria; ABS15237; ABS15237; Oant_2524.
DR KEGG; oan:Oant_2524; -.
DR PATRIC; fig|439375.7.peg.2657; -.
DR eggNOG; COG3808; Bacteria.
DR HOGENOM; CLU_008743_3_1_5; -.
DR OMA; MATTAMQ; -.
DR OrthoDB; 98036at2; -.
DR PhylomeDB; Q8VRZ2; -.
DR Proteomes; UP000002301; Chromosome 1.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR GO; GO:0004427; F:inorganic diphosphatase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0009678; F:pyrophosphate hydrolysis-driven proton transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR HAMAP; MF_01129; PPase_energized_pump; 1.
DR InterPro; IPR004131; PPase-energised_H-pump.
DR PANTHER; PTHR31998; PTHR31998; 1.
DR Pfam; PF03030; H_PPase; 1.
DR PIRSF; PIRSF001265; H+-PPase; 1.
DR TIGRFAMs; TIGR01104; V_PPase; 1.
PE 3: Inferred from homology;
KW Calcium; Cell inner membrane; Cell membrane; Hydrogen ion transport;
KW Ion transport; Magnesium; Membrane; Metal-binding; Reference proteome;
KW Translocase; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..718
FT /note="K(+)-insensitive pyrophosphate-energized proton
FT pump"
FT /id="PRO_0000217023"
FT TRANSMEM 6..26
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01129"
FT TRANSMEM 54..76
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01129"
FT TRANSMEM 81..103
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01129"
FT TRANSMEM 112..132
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01129"
FT TRANSMEM 133..153
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01129"
FT TRANSMEM 168..188
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01129"
FT TRANSMEM 240..260
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01129"
FT TRANSMEM 265..285
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01129"
FT TRANSMEM 300..320
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01129"
FT TRANSMEM 335..355
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01129"
FT TRANSMEM 385..405
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01129"
FT TRANSMEM 413..433
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01129"
FT TRANSMEM 472..492
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01129"
FT TRANSMEM 524..544
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01129"
FT TRANSMEM 593..613
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01129"
FT TRANSMEM 620..640
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01129"
FT TRANSMEM 695..715
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01129"
FT BINDING 190
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 193
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 197
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 220
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 223
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 441
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 650
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT BINDING 682
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT BINDING 686
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT BINDING 689
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT SITE 182
FT /note="Important for ion transport"
FT /evidence="ECO:0000250"
FT SITE 227
FT /note="Important for ion transport"
FT /evidence="ECO:0000250"
FT SITE 234
FT /note="Important for ion transport"
FT /evidence="ECO:0000250"
FT SITE 471
FT /note="Determinant of potassium independence"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01129"
FT SITE 690
FT /note="Important for ion transport"
FT /evidence="ECO:0000250"
FT SITE 701
FT /note="Important for ion transport"
FT /evidence="ECO:0000250"
FT CONFLICT 259
FT /note="I -> V (in Ref. 2; AAL69330)"
FT /evidence="ECO:0000305"
FT CONFLICT 288..290
FT /note="GVN -> SVD (in Ref. 2; AAL69330)"
FT /evidence="ECO:0000305"
FT CONFLICT 314
FT /note="I -> V (in Ref. 2; AAL69330)"
FT /evidence="ECO:0000305"
FT CONFLICT 320
FT /note="V -> I (in Ref. 2; AAL69330)"
FT /evidence="ECO:0000305"
FT CONFLICT 340
FT /note="L -> I (in Ref. 2; AAL69330)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 718 AA; 73272 MW; 478B489906207833 CRC64;
MQMGVAVLVL VIACGVVSVL FAIWAIRSVL AADQGTQRMQ EIAEAIREGA SAYLTRQYST
IAIVGVVVFL AAWYLLSISA AIGFLIGAVL SGVTGFIGMH VSVRANVRTA QAASLSLAGG
LELAFKSGAI TGLLVAGLAL LGVSVYYFIL TVWLGYSPSD RTVIDALVSL GFGASLISIF
ARLGGGIFTK GADVGGDLVG KVEAGIPEDD PRNPATIADN VGDNVGDCAG MAADLFETYA
VTVVATMVLG AIFFNGSDIL SSVMLYPLMI CGACVITSIV GTFFVKLGVN GSIMGALYKG
LIATGLLSIV GLAIANTLTV GWGEIGTVAG KSITGTNLFL CGLIGLIVTG LIVVITEYYT
GTNKRPVNSI AQASVTGHGT NVIQGLAVSL ESTALPAIVI VGGIISTYQL AGLFGTAIAV
TAMLGIAGMI VALDAFGPVT DNAGGIAEMA GLDPEVRKAT DALDAVGNTT KAVTKGYAIG
SAGLGALVLF AAYSNDLAYF AANGQTYPYF ADMGPVSFDL SNPYVVAGLI FGGLIPYLFG
GMAMTAVGRA GGAVVQEVRR QFREKPGIMT GKERPDYARA VDLLTRAAIR EMIIPSLLPV
LAPIVVYFGV LLISGSKAAA FAALGASLLG VIVNGLFVAI SMTSGGGAWD NAKKSFEDGF
TDADGVKHLK GSEAHKASVT GDTVGDPYKD TAGPAVNPAI KITNIVALLL LAVLAHMS
