HPPA_CHLAA
ID HPPA_CHLAA Reviewed; 775 AA.
AC Q8VNW3; A9W9P8;
DT 25-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 18-MAR-2008, sequence version 2.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=K(+)-insensitive pyrophosphate-energized proton pump {ECO:0000255|HAMAP-Rule:MF_01129};
DE EC=7.1.3.1 {ECO:0000255|HAMAP-Rule:MF_01129};
DE AltName: Full=Membrane-bound proton-translocating pyrophosphatase {ECO:0000255|HAMAP-Rule:MF_01129};
DE AltName: Full=Pyrophosphate-energized inorganic pyrophosphatase {ECO:0000255|HAMAP-Rule:MF_01129};
DE Short=H(+)-PPase {ECO:0000255|HAMAP-Rule:MF_01129};
GN Name=hppA {ECO:0000255|HAMAP-Rule:MF_01129}; Synonyms=vppA;
GN OrderedLocusNames=Caur_1306;
OS Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl).
OC Bacteria; Chloroflexi; Chloroflexia; Chloroflexales; Chloroflexineae;
OC Chloroflexaceae; Chloroflexus.
OX NCBI_TaxID=324602;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29366 / DSM 635 / J-10-fl;
RX PubMed=21714912; DOI=10.1186/1471-2164-12-334;
RA Tang K.H., Barry K., Chertkov O., Dalin E., Han C.S., Hauser L.J.,
RA Honchak B.M., Karbach L.E., Land M.L., Lapidus A., Larimer F.W.,
RA Mikhailova N., Pitluck S., Pierson B.K., Blankenship R.E.;
RT "Complete genome sequence of the filamentous anoxygenic phototrophic
RT bacterium Chloroflexus aurantiacus.";
RL BMC Genomics 12:334-334(2011).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 474-673.
RA Perez-Castineira J.R., Losada M., Serrano A.;
RT "Evidence for a wide distribution of proton-translocating pyrophosphatases
RT among photosynthetic organisms.";
RL Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Proton pump that utilizes the energy of pyrophosphate
CC hydrolysis as the driving force for proton movement across the
CC membrane. Generates a proton motive force. {ECO:0000255|HAMAP-
CC Rule:MF_01129}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=diphosphate + H(+)(in) + H2O = 2 H(+)(out) + 2 phosphate;
CC Xref=Rhea:RHEA:13973, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:43474; EC=7.1.3.1;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01129};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01129};
CC -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01129}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_01129};
CC Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_01129}.
CC -!- SIMILARITY: Belongs to the H(+)-translocating pyrophosphatase (TC
CC 3.A.10) family. K(+)-insensitive subfamily. {ECO:0000255|HAMAP-
CC Rule:MF_01129}.
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DR EMBL; CP000909; ABY34534.1; -; Genomic_DNA.
DR EMBL; AJ251370; CAC80905.1; -; Genomic_DNA.
DR RefSeq; WP_012257190.1; NC_010175.1.
DR RefSeq; YP_001634923.1; NC_010175.1.
DR AlphaFoldDB; Q8VNW3; -.
DR SMR; Q8VNW3; -.
DR STRING; 324602.Caur_1306; -.
DR EnsemblBacteria; ABY34534; ABY34534; Caur_1306.
DR KEGG; cau:Caur_1306; -.
DR PATRIC; fig|324602.8.peg.1495; -.
DR eggNOG; COG3808; Bacteria.
DR HOGENOM; CLU_008743_3_1_0; -.
DR InParanoid; Q8VNW3; -.
DR OMA; MATTAMQ; -.
DR Proteomes; UP000002008; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR GO; GO:0004427; F:inorganic diphosphatase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0009678; F:pyrophosphate hydrolysis-driven proton transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR HAMAP; MF_01129; PPase_energized_pump; 1.
DR InterPro; IPR004131; PPase-energised_H-pump.
DR PANTHER; PTHR31998; PTHR31998; 1.
DR Pfam; PF03030; H_PPase; 1.
DR PIRSF; PIRSF001265; H+-PPase; 1.
DR TIGRFAMs; TIGR01104; V_PPase; 1.
PE 3: Inferred from homology;
KW Calcium; Cell membrane; Hydrogen ion transport; Ion transport; Magnesium;
KW Membrane; Metal-binding; Reference proteome; Translocase; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..775
FT /note="K(+)-insensitive pyrophosphate-energized proton
FT pump"
FT /id="PRO_0000217014"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01129"
FT TRANSMEM 65..85
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01129"
FT TRANSMEM 108..128
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01129"
FT TRANSMEM 160..180
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01129"
FT TRANSMEM 185..205
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01129"
FT TRANSMEM 259..279
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01129"
FT TRANSMEM 288..308
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01129"
FT TRANSMEM 327..347
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01129"
FT TRANSMEM 359..379
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01129"
FT TRANSMEM 413..433
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01129"
FT TRANSMEM 442..462
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01129"
FT TRANSMEM 508..528
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01129"
FT TRANSMEM 555..575
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01129"
FT TRANSMEM 622..642
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01129"
FT TRANSMEM 644..664
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01129"
FT TRANSMEM 710..730
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01129"
FT TRANSMEM 735..755
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01129"
FT BINDING 208
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 211
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 215
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 241
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 472
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 671
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT BINDING 697
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT BINDING 701
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT BINDING 704
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT SITE 200
FT /note="Important for ion transport"
FT /evidence="ECO:0000250"
FT SITE 245
FT /note="Important for ion transport"
FT /evidence="ECO:0000250"
FT SITE 252
FT /note="Important for ion transport"
FT /evidence="ECO:0000250"
FT SITE 502
FT /note="Determinant of potassium independence"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01129"
FT SITE 705
FT /note="Important for ion transport"
FT /evidence="ECO:0000250"
FT SITE 716
FT /note="Important for ion transport"
FT /evidence="ECO:0000250"
SQ SEQUENCE 775 AA; 80486 MW; 509F7B985C75D931 CRC64;
MQDLNAVQSL AVWAVLVISL LGIGYAFFIR SQILAQDTGT PKMREVWGFI KTGANAYLSQ
QFRTISILIV ILTFVLAASV FIIPPTTEAV ERFGSKEAAT IWVAIGRAVA FLMGSLFSYA
VGFVGMNVAV EGNVRVAAAA RKGYNPALQV AYKSGSVTGM LTVGLGLLGG TLIFMVFGIA
APDALLGFGF GGSLIALFMR VGGGIYTKAA DVGADLVGKV EAGIPEDDPR NAAVIADLVG
DNVGDCAGMA ADVFESFEVT LVSALILGLV LGDAVVGTIG DGAYDLRFII FPLVLRAIGV
VASVIGNLFV TTDERKRNAM AAMNRGFYIA AGLAIAASAA ATPVFMVNEA TGEVDWRPFF
ATLSGVVLAI VLDKLTEYFT STHFSPVKET SKASQTGSAT NILSGLALGM ESSVWAILVI
SASIFTSVLI YAGEPAATQF TAILYGVSLT GIGMLLLTGN TISMDSFGPI SDNANGIGEM
AGLDKNARNV MDDLDAVGNT TKAVTKGIAI GSAVIAAVAL YGSYFTDVNK VLQQMINEGR
QGIELLASIN VAAPPVFIGL LIGGAVPFLF SALTIRAVSR AAAQIVNEVR RQFRIPGLME
GKVQPDYARA VQISTTAAQK ELISLGLIAV MVPIIVGFTL GVEALGGFLA GIILTGQLMA
VFQANAGGAW DNAKKYIEEG NFGGKHSEPH KAAVVGDTVG DPLKDTAGPA LNPMIKVINL
VALIIAPIVV TIEPGSPGVI IAMIICGAAL VWAIWQSKRE SEALKEIAQA PASAD
