ID   HPPA_HELCL              Reviewed;         197 AA.
AC   Q8VNJ8;
DT   25-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   25-MAY-2022, entry version 65.
DE   RecName: Full=Putative K(+)-stimulated pyrophosphate-energized sodium pump;
DE            EC=7.2.3.-;
DE   AltName: Full=Membrane-bound sodium-translocating pyrophosphatase;
DE   AltName: Full=Pyrophosphate-energized inorganic pyrophosphatase;
DE            Short=Na(+)-PPase;
DE   Flags: Fragment;
GN   Name=hppA; Synonyms=vppA;
OS   Heliobacterium chlorum.
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Heliobacteriaceae;
OC   Heliobacterium.
OX   NCBI_TaxID=2698;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 35205 / DSM 3682 / Gest/Favinger;
RA   Perez-Castineira J.R., Losada M., Serrano A.;
RT   "Evidence for a wide distribution of proton-translocating pyrophosphatases
RT   among photosynthetic organisms.";
RL   Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Sodium pump that utilizes the energy of pyrophosphate
CC       hydrolysis as the driving force for Na(+) movement across the membrane.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=diphosphate + H2O + Na(+)(in) = H(+) + Na(+)(out) + 2
CC         phosphate; Xref=Rhea:RHEA:57884, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:29101, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:43474;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- ACTIVITY REGULATION: Requires K(+) for maximal activity. {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC       protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the H(+)-translocating pyrophosphatase (TC
CC       3.A.10) family. K(+)-stimulated subfamily. {ECO:0000305}.
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DR   EMBL; AJ251371; CAC80906.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q8VNJ8; -.
DR   SMR; Q8VNJ8; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0004427; F:inorganic diphosphatase activity; IEA:InterPro.
DR   GO; GO:0009678; F:pyrophosphate hydrolysis-driven proton transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR   InterPro; IPR004131; PPase-energised_H-pump.
DR   PANTHER; PTHR31998; PTHR31998; 1.
DR   Pfam; PF03030; H_PPase; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Ion transport; Magnesium; Membrane; Potassium; Sodium;
KW   Sodium transport; Translocase; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           <1..>197
FT                   /note="Putative K(+)-stimulated pyrophosphate-energized
FT                   sodium pump"
FT                   /id="PRO_0000217004"
FT   TRANSMEM        31..51
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        79..99
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        146..166
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        168..188
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   SITE            29
FT                   /note="Determinant of potassium dependence"
FT                   /evidence="ECO:0000250"
FT   NON_TER         1
FT   NON_TER         197
SQ   SEQUENCE   197 AA;  20230 MW;  DA4FC7F465481D6E CRC64;
     AGGIAEMAEL GPEVRKKTDK LDAVGNTTAA VAKGFAIGSA ALTALALFTA FAEEVVKSPG
     VSSALHGSHF VLNLLEPKII IGLFIGGTVP FLFCAFAMEA VGKAAFEMIG EVRRQFREIP
     GLMEGKAKPD YARCVDISTR AAIRQMIAPG LLAVITPLIV GFGFGAQALG GMLAGVTVAG
     VLLAIFMANA GGAWDHA