ID   HPPA_MYXXA              Reviewed;         275 AA.
AC   Q93NB7;
DT   25-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   25-MAY-2022, entry version 68.
DE   RecName: Full=Putative K(+)-stimulated pyrophosphate-energized sodium pump;
DE            EC=7.2.3.-;
DE   AltName: Full=Membrane-bound sodium-translocating pyrophosphatase;
DE   AltName: Full=Pyrophosphate-energized inorganic pyrophosphatase;
DE            Short=Na(+)-PPase;
DE   Flags: Fragment;
GN   Name=hppA;
OS   Myxococcus xanthus.
OC   Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales;
OC   Cystobacterineae; Myxococcaceae; Myxococcus.
OX   NCBI_TaxID=34;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=DZF1;
RX   PubMed=11087177; DOI=10.1089/10906590050179783;
RA   Inouye S., Jain R., Ueki T., Nariya H., Xu C.Y., Hsu M.Y.,
RA   Fernandez-Luque B.A., Munoz-Dorado J., Farez-Vidal E., Inouye M.;
RT   "A large family of eukaryotic-like protein Ser/Thr kinases of Myxococcus
RT   xanthus, a developmental bacterium.";
RL   Microb. Comp. Genomics 5:103-120(2000).
CC   -!- FUNCTION: Sodium pump that utilizes the energy of pyrophosphate
CC       hydrolysis as the driving force for Na(+) movement across the membrane.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=diphosphate + H2O + Na(+)(in) = H(+) + Na(+)(out) + 2
CC         phosphate; Xref=Rhea:RHEA:57884, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:29101, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:43474;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- ACTIVITY REGULATION: Requires K(+) for maximal activity. {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the H(+)-translocating pyrophosphatase (TC
CC       3.A.10) family. K(+)-stimulated subfamily. {ECO:0000305}.
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DR   EMBL; AF377340; AAK64453.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q93NB7; -.
DR   SMR; Q93NB7; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0004427; F:inorganic diphosphatase activity; IEA:InterPro.
DR   GO; GO:0009678; F:pyrophosphate hydrolysis-driven proton transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR   InterPro; IPR004131; PPase-energised_H-pump.
DR   PANTHER; PTHR31998; PTHR31998; 1.
DR   Pfam; PF03030; H_PPase; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Ion transport; Magnesium; Membrane;
KW   Potassium; Sodium; Sodium transport; Translocase; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           <1..275
FT                   /note="Putative K(+)-stimulated pyrophosphate-energized
FT                   sodium pump"
FT                   /id="PRO_0000217021"
FT   TRANSMEM        51..71
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        96..116
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        163..182
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        189..211
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        254..274
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   SITE            49
FT                   /note="Determinant of potassium dependence"
FT                   /evidence="ECO:0000250"
FT   NON_TER         1
SQ   SEQUENCE   275 AA;  27791 MW;  E4BA754C13706146 CRC64;
     MLGGTAVVMT VDAYGPISDN AGGISEMSGL GPEVRAITDE LDAVGNTTAA IGKGFAIGSA
     TLTVIALFSA FNLEVNHTRI AAGLPEMSLQ LTNPNVIVGL LLGSILPFLV GASTMLAVGR
     AAGAIVEEIG RQFREIPGLM ELKADPDPKK IVDIATKSAL QEMVFPGIIA VAAPPLVGYL
     LGPGALAGLL AGSLVVGATM ALYMANAGGA WDNAKKFIEK GKLPGHAKGS AVHKAAVVGD
     MVGDPFKDTS GPGVAILIKV MSVVSLLVAS LIALR