HPPA_RHIRD
ID HPPA_RHIRD Reviewed; 189 AA.
AC Q8VPZ0;
DT 25-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 70.
DE RecName: Full=Putative K(+)-stimulated pyrophosphate-energized sodium pump;
DE EC=7.2.3.-;
DE AltName: Full=Membrane-bound sodium-translocating pyrophosphatase;
DE AltName: Full=Pyrophosphate-energized inorganic pyrophosphatase;
DE Short=Na(+)-PPase;
DE Flags: Fragment;
GN Name=hppA; Synonyms=vppA;
OS Rhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium
OS radiobacter).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC Agrobacterium tumefaciens complex.
OX NCBI_TaxID=358;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=D44;
RA Perez-Castineira J.R., Losada M., Serrano A.;
RT "Presence of proton-translocating pyrophosphatase genes in root nodule-
RT making bacteria (Rhizobia) and pathogenic tumour-making bacteria
RT (Agrobacterium).";
RL Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Sodium pump that utilizes the energy of pyrophosphate
CC hydrolysis as the driving force for Na(+) movement across the membrane.
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=diphosphate + H2O + Na(+)(in) = H(+) + Na(+)(out) + 2
CC phosphate; Xref=Rhea:RHEA:57884, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:29101, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:43474;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- ACTIVITY REGULATION: Requires K(+) for maximal activity. {ECO:0000250}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the H(+)-translocating pyrophosphatase (TC
CC 3.A.10) family. K(+)-stimulated subfamily. {ECO:0000305}.
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DR EMBL; AJ251785; CAC80979.1; -; Genomic_DNA.
DR AlphaFoldDB; Q8VPZ0; -.
DR SMR; Q8VPZ0; -.
DR PRIDE; Q8VPZ0; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004427; F:inorganic diphosphatase activity; IEA:InterPro.
DR GO; GO:0009678; F:pyrophosphate hydrolysis-driven proton transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR InterPro; IPR004131; PPase-energised_H-pump.
DR PANTHER; PTHR31998; PTHR31998; 1.
DR Pfam; PF03030; H_PPase; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Ion transport; Magnesium; Membrane;
KW Potassium; Sodium; Sodium transport; Translocase; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN <1..>189
FT /note="Putative K(+)-stimulated pyrophosphate-energized
FT sodium pump"
FT /id="PRO_0000217008"
FT TRANSMEM 31..51
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 78..98
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 146..166
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 168..188
FT /note="Helical"
FT /evidence="ECO:0000255"
FT SITE 29
FT /note="Determinant of potassium dependence"
FT /evidence="ECO:0000250"
FT NON_TER 1
FT NON_TER 189
SQ SEQUENCE 189 AA; 19439 MW; E8CEB6C2DD06A6CD CRC64;
AGGIAEMAEL GPEVRKTTDK LDSVGNTTAA IAKGFAIGSA ALTALALFTA FAEEIAKNPK
LTGLLVDGHL VVNLTEPGVI IGIFLGATLP FLVCAFTMEA VGKAAFEMIE EVRRQFREIP
GIMEGTGRPD YAKCVDISTK AAIREMMLPG VFAVGAPLIV GFLLGAKALA GFLAGVTASG
VLLAIFSPS
