ID   3SUC2_NAJKA             Reviewed;          65 AA.
AC   P82463;
DT   11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2000, sequence version 1.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=Muscarinic toxin-like protein 2 {ECO:0000303|PubMed:11082188};
DE            Short=MTLP-2 {ECO:0000303|PubMed:11082188};
OS   Naja kaouthia (Monocled cobra) (Naja siamensis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Elapidae; Elapinae; Naja.
OX   NCBI_TaxID=8649;
RN   [1]
RP   PROTEIN SEQUENCE, MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RC   TISSUE=Venom;
RX   PubMed=11082188; DOI=10.1046/j.1432-1033.2000.01775.x;
RA   Kukhtina V.V., Weise C., Muranova T.A., Starkov V.G., Franke P., Hucho F.,
RA   Wnendt S., Gillen C., Tsetlin V.I., Utkin Y.N.;
RT   "Muscarinic toxin-like proteins from cobra venom.";
RL   Eur. J. Biochem. 267:6784-6789(2000).
CC   -!- SUBUNIT: Monomer. {ECO:0000305|PubMed:11082188}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:11082188}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=7293; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:11082188};
CC   -!- MISCELLANEOUS: Has no effect on ligand binding to any of the mAChR
CC       subtypes at concentrations up to 1 uM. {ECO:0000269|PubMed:11082188}.
CC   -!- MISCELLANEOUS: Is classified as a P-type cytotoxin, since a proline
CC       residue stands at position 33 (Pro-31 in standard classification).
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the snake three-finger toxin family. Short-chain
CC       subfamily. Type C muscarinic toxin sub-subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P82463; -.
DR   SMR; P82463; -.
DR   PRIDE; P82463; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   CDD; cd00206; snake_toxin; 1.
DR   Gene3D; 2.10.60.10; -; 1.
DR   InterPro; IPR003572; Cytotoxin_Cobra.
DR   InterPro; IPR003571; Snake_3FTx.
DR   InterPro; IPR045860; Snake_toxin-like_sf.
DR   InterPro; IPR018354; Snake_toxin_con_site.
DR   InterPro; IPR035076; Toxin/TOLIP.
DR   Pfam; PF00087; Toxin_TOLIP; 1.
DR   PRINTS; PR00282; CYTOTOXIN.
DR   SUPFAM; SSF57302; SSF57302; 1.
DR   PROSITE; PS00272; SNAKE_TOXIN; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Neurotoxin;
KW   Postsynaptic neurotoxin; Secreted; Toxin.
FT   CHAIN           1..65
FT                   /note="Muscarinic toxin-like protein 2"
FT                   /evidence="ECO:0000269|PubMed:11082188"
FT                   /id="PRO_0000093651"
FT   DISULFID        3..24
FT                   /evidence="ECO:0000250|UniProtKB:P60301"
FT   DISULFID        17..42
FT                   /evidence="ECO:0000250|UniProtKB:P60301"
FT   DISULFID        46..57
FT                   /evidence="ECO:0000250|UniProtKB:P60301"
FT   DISULFID        58..63
FT                   /evidence="ECO:0000250|UniProtKB:P60301"
SQ   SEQUENCE   65 AA;  7298 MW;  8789C92D9C67AF6F CRC64;
     LTCVKEKSIF GVTTEDCPDG QNLCFKRWHM IVPGRYKKTR GCAATCPIAE NRDVIECCST
     DKCNL