HPPD_XENTR
ID HPPD_XENTR Reviewed; 394 AA.
AC Q5BKL0;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 12-APR-2005, sequence version 1.
DT 03-AUG-2022, entry version 90.
DE RecName: Full=4-hydroxyphenylpyruvate dioxygenase;
DE EC=1.13.11.27 {ECO:0000250|UniProtKB:P32755};
DE AltName: Full=4-hydroxyphenylpyruvic acid oxidase;
DE Short=4HPPD;
DE Short=HPD;
DE Short=HPPDase;
GN Name=hpd;
OS Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX NCBI_TaxID=8364;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the conversion of 4-hydroxyphenylpyruvic acid to
CC homogentisic acid, one of the steps in tyrosine catabolism.
CC {ECO:0000250|UniProtKB:P32755}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-(4-hydroxyphenyl)pyruvate + O2 = CO2 + homogentisate;
CC Xref=Rhea:RHEA:16189, ChEBI:CHEBI:15379, ChEBI:CHEBI:16169,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:36242; EC=1.13.11.27;
CC Evidence={ECO:0000250|UniProtKB:P32755};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16190;
CC Evidence={ECO:0000250|UniProtKB:P32755};
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC Evidence={ECO:0000250|UniProtKB:P32755};
CC Note=Binds 1 Fe cation per subunit. {ECO:0000250|UniProtKB:P32755};
CC -!- PATHWAY: Amino-acid degradation; L-phenylalanine degradation;
CC acetoacetate and fumarate from L-phenylalanine: step 3/6.
CC -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:P32755}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P32755}.
CC Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:P32755};
CC Peripheral membrane protein {ECO:0000250|UniProtKB:P32755}. Golgi
CC apparatus membrane {ECO:0000250|UniProtKB:P32755}; Peripheral membrane
CC protein {ECO:0000250|UniProtKB:P32755}.
CC -!- SIMILARITY: Belongs to the 4HPPD family. {ECO:0000305}.
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DR EMBL; BC091035; AAH91035.1; -; mRNA.
DR RefSeq; NP_001025588.1; NM_001030417.1.
DR AlphaFoldDB; Q5BKL0; -.
DR SMR; Q5BKL0; -.
DR STRING; 8364.ENSXETP00000062603; -.
DR PaxDb; Q5BKL0; -.
DR PRIDE; Q5BKL0; -.
DR DNASU; 594976; -.
DR Ensembl; ENSXETT00000085370; ENSXETP00000064696; ENSXETG00000007789.
DR GeneID; 594976; -.
DR KEGG; xtr:594976; -.
DR CTD; 594976; -.
DR Xenbase; XB-GENE-986330; hpd-like.2.
DR eggNOG; KOG0638; Eukaryota.
DR HOGENOM; CLU_034004_3_1_1; -.
DR InParanoid; Q5BKL0; -.
DR OrthoDB; 1087836at2759; -.
DR PhylomeDB; Q5BKL0; -.
DR UniPathway; UPA00139; UER00362.
DR Proteomes; UP000008143; Chromosome 2.
DR Proteomes; UP000790000; Unplaced.
DR Bgee; ENSXETG00000007789; Expressed in liver and 7 other tissues.
DR ExpressionAtlas; Q5BKL0; baseline and differential.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0000139; C:Golgi membrane; IBA:GO_Central.
DR GO; GO:0003868; F:4-hydroxyphenylpyruvate dioxygenase activity; ISS:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR GO; GO:0006559; P:L-phenylalanine catabolic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0006572; P:tyrosine catabolic process; ISS:UniProtKB.
DR CDD; cd07250; HPPD_C_like; 1.
DR CDD; cd08342; HPPD_N_like; 1.
DR Gene3D; 3.10.180.10; -; 2.
DR InterPro; IPR005956; 4OHPhenylPyrv_dOase.
DR InterPro; IPR041735; 4OHPhenylPyrv_dOase_C.
DR InterPro; IPR041736; 4OHPhenylPyrv_dOase_N.
DR InterPro; IPR029068; Glyas_Bleomycin-R_OHBP_Dase.
DR InterPro; IPR004360; Glyas_Fos-R_dOase_dom.
DR InterPro; IPR037523; VOC.
DR PANTHER; PTHR11959; PTHR11959; 1.
DR Pfam; PF00903; Glyoxalase; 2.
DR PIRSF; PIRSF009283; HPP_dOase; 1.
DR SUPFAM; SSF54593; SSF54593; 1.
DR TIGRFAMs; TIGR01263; 4HPPD; 1.
DR PROSITE; PS51819; VOC; 2.
PE 2: Evidence at transcript level;
KW Cytoplasm; Dioxygenase; Endoplasmic reticulum; Golgi apparatus; Iron;
KW Membrane; Metal-binding; Oxidoreductase; Phenylalanine catabolism;
KW Reference proteome; Repeat; Tyrosine catabolism.
FT CHAIN 1..394
FT /note="4-hydroxyphenylpyruvate dioxygenase"
FT /id="PRO_0000088393"
FT DOMAIN 18..149
FT /note="VOC 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01163"
FT DOMAIN 181..339
FT /note="VOC 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01163"
FT BINDING 184
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250|UniProtKB:P32755"
FT BINDING 267
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250|UniProtKB:P32755"
FT BINDING 350
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250|UniProtKB:P32755"
SQ SEQUENCE 394 AA; 44989 MW; A0347A1DE03EC07C CRC64;
MTSYTDKGEK HARGRFLSFH HLTFWVGNAK QAASFYCDKF GFEPCAYKGL ETGSRDVVSH
AIKQDKIIFV FQSPLNPGNQ EMGQHMIKHG DGVKDVAFQV EDCDFLFQKA KDHGAVVVRE
PWIEEDEGGK VKYAVLQTYG DTTHTLLEYL GPYRGVFLPG YKEPLFRDPL LPTLPSGCLS
FIDHIVGNQP DNEMVPIVEW YQKCLLFHRF WSVDDKQIHT EYSSLRSIVV TNYEETIKMP
INEPAAGKKK SQIQEYVDYY GSAGVQHIAL NTSNIIKAVK NLKSRGIEFL SAPDTYYEEL
RKKLKTAKIT VKEDLNVLQE LKILVDYDDK GYLLQIFTKP MQDRPTLFLE VIQRYNHFGF
GAGNFKSLFE AIETDQDARG NLTIYAANGE HQVL
