ID   HPPK_METEA              Reviewed;         158 AA.
AC   P71512; C5B123;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   22-SEP-2009, sequence version 2.
DT   03-AUG-2022, entry version 120.
DE   RecName: Full=2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase {ECO:0000250|UniProtKB:P26281};
DE            EC=2.7.6.3 {ECO:0000250|UniProtKB:P26281};
DE   AltName: Full=6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase {ECO:0000250|UniProtKB:P26281};
DE            Short=PPPK {ECO:0000250|UniProtKB:P26281};
DE   AltName: Full=7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase {ECO:0000250|UniProtKB:P26281};
DE            Short=HPPK {ECO:0000250|UniProtKB:P26281};
GN   Name=folK; Synonyms=folA; OrderedLocusNames=MexAM1_META1p1743;
OS   Methylorubrum extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB
OS   9133 / AM1) (Methylobacterium extorquens).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Methylobacteriaceae; Methylorubrum.
OX   NCBI_TaxID=272630;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9168622; DOI=10.1099/00221287-143-5-1729;
RA   Chistoserdova L.V., Lidstrom M.E.;
RT   "Molecular and mutational analysis of a DNA region separating two
RT   methylotrophy gene clusters in Methylobacterium extorquens AM1.";
RL   Microbiology 143:1729-1736(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1;
RX   PubMed=19440302; DOI=10.1371/journal.pone.0005584;
RA   Vuilleumier S., Chistoserdova L., Lee M.-C., Bringel F., Lajus A., Zhou Y.,
RA   Gourion B., Barbe V., Chang J., Cruveiller S., Dossat C., Gillett W.,
RA   Gruffaz C., Haugen E., Hourcade E., Levy R., Mangenot S., Muller E.,
RA   Nadalig T., Pagni M., Penny C., Peyraud R., Robinson D.G., Roche D.,
RA   Rouy Z., Saenampechek C., Salvignol G., Vallenet D., Wu Z., Marx C.J.,
RA   Vorholt J.A., Olson M.V., Kaul R., Weissenbach J., Medigue C.,
RA   Lidstrom M.E.;
RT   "Methylobacterium genome sequences: a reference blueprint to investigate
RT   microbial metabolism of C1 compounds from natural and industrial sources.";
RL   PLoS ONE 4:E5584-E5584(2009).
CC   -!- FUNCTION: Catalyzes the transfer of pyrophosphate from adenosine
CC       triphosphate (ATP) to 6-hydroxymethyl-7,8-dihydropterin, an enzymatic
CC       step in folate biosynthesis pathway. {ECO:0000250|UniProtKB:P26281}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=6-hydroxymethyl-7,8-dihydropterin + ATP = (7,8-dihydropterin-
CC         6-yl)methyl diphosphate + AMP + H(+); Xref=Rhea:RHEA:11412,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:44841,
CC         ChEBI:CHEBI:72950, ChEBI:CHEBI:456215; EC=2.7.6.3;
CC         Evidence={ECO:0000250|UniProtKB:P26281};
CC   -!- PATHWAY: Cofactor biosynthesis; tetrahydrofolate biosynthesis; 2-amino-
CC       4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-
CC       dihydroneopterin triphosphate: step 4/4.
CC       {ECO:0000250|UniProtKB:P26281}.
CC   -!- SIMILARITY: Belongs to the HPPK family. {ECO:0000305}.
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DR   EMBL; U72662; AAB58893.1; -; Genomic_DNA.
DR   EMBL; CP001510; ACS39587.1; -; Genomic_DNA.
DR   RefSeq; WP_003597615.1; NC_012808.1.
DR   AlphaFoldDB; P71512; -.
DR   SMR; P71512; -.
DR   STRING; 272630.MexAM1_META1p1743; -.
DR   PRIDE; P71512; -.
DR   EnsemblBacteria; ACS39587; ACS39587; MexAM1_META1p1743.
DR   KEGG; mea:Mex_1p1743; -.
DR   eggNOG; COG0801; Bacteria.
DR   HOGENOM; CLU_097916_2_3_5; -.
DR   OMA; QVILRQN; -.
DR   OrthoDB; 1953248at2; -.
DR   UniPathway; UPA00077; UER00155.
DR   Proteomes; UP000009081; Chromosome.
DR   GO; GO:0003848; F:2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046656; P:folic acid biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   GO; GO:0046654; P:tetrahydrofolate biosynthetic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd00483; HPPK; 1.
DR   Gene3D; 3.30.70.560; -; 1.
DR   InterPro; IPR000550; Hppk.
DR   InterPro; IPR035907; Hppk_sf.
DR   Pfam; PF01288; HPPK; 1.
DR   SUPFAM; SSF55083; SSF55083; 1.
DR   TIGRFAMs; TIGR01498; folK; 1.
DR   PROSITE; PS00794; HPPK; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Folate biosynthesis; Kinase; Nucleotide-binding; Transferase.
FT   CHAIN           1..158
FT                   /note="2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
FT                   pyrophosphokinase"
FT                   /id="PRO_0000168253"
FT   CONFLICT        87
FT                   /note="R -> P (in Ref. 1; AAB58893)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   158 AA;  17206 MW;  0C56DD5EEB13B7CD CRC64;
     MTRAYLGLGS NIGDKAAMLA GAVEHLAATP GIRVVARSAD YRTPPWGDTD QDWFLNAAVA
     IDTELTPHGL LEVCLSIEAA LGRVRERRWG PRVIDIDVLA YEGAQVSDER LVLPHRFVRE
     RAFVLVPLAE IAPDLVIGGE TVREALAKLD PSGIERVE