HPRT_METAR
ID HPRT_METAR Reviewed; 191 AA.
AC Q0W496;
DT 22-FEB-2012, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 1.
DT 25-MAY-2022, entry version 73.
DE RecName: Full=Hypoxanthine/guanine phosphoribosyltransferase {ECO:0000255|HAMAP-Rule:MF_01467};
DE Short=HGPRTase {ECO:0000255|HAMAP-Rule:MF_01467};
DE EC=2.4.2.8 {ECO:0000255|HAMAP-Rule:MF_01467};
GN Name=hpt {ECO:0000255|HAMAP-Rule:MF_01467}; OrderedLocusNames=UNCMA_14320;
GN ORFNames=RCIX1545;
OS Methanocella arvoryzae (strain DSM 22066 / NBRC 105507 / MRE50).
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanocellales; Methanocellaceae; Methanocella.
OX NCBI_TaxID=351160;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 22066 / NBRC 105507 / MRE50;
RX PubMed=16857943; DOI=10.1126/science.1127062;
RA Erkel C., Kube M., Reinhardt R., Liesack W.;
RT "Genome of rice cluster I archaea -- the key methane producers in the rice
RT rhizosphere.";
RL Science 313:370-372(2006).
CC -!- FUNCTION: Catalyzes a salvage reaction resulting in the formation of
CC IMP that is energically less costly than de novo synthesis.
CC {ECO:0000255|HAMAP-Rule:MF_01467}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=diphosphate + IMP = 5-phospho-alpha-D-ribose 1-diphosphate +
CC hypoxanthine; Xref=Rhea:RHEA:17973, ChEBI:CHEBI:17368,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:58017, ChEBI:CHEBI:58053; EC=2.4.2.8;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01467};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=diphosphate + GMP = 5-phospho-alpha-D-ribose 1-diphosphate +
CC guanine; Xref=Rhea:RHEA:25424, ChEBI:CHEBI:16235, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:58017, ChEBI:CHEBI:58115; EC=2.4.2.8;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01467};
CC -!- PATHWAY: Purine metabolism; IMP biosynthesis via salvage pathway; IMP
CC from hypoxanthine: step 1/1. {ECO:0000255|HAMAP-Rule:MF_01467}.
CC -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01467}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01467}.
CC -!- SIMILARITY: Belongs to the purine/pyrimidine phosphoribosyltransferase
CC family. Archaeal HPRT subfamily. {ECO:0000255|HAMAP-Rule:MF_01467}.
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DR EMBL; AM114193; CAJ36797.1; -; Genomic_DNA.
DR RefSeq; WP_012035760.1; NC_009464.1.
DR AlphaFoldDB; Q0W496; -.
DR SMR; Q0W496; -.
DR STRING; 351160.RCIX1545; -.
DR EnsemblBacteria; CAJ36797; CAJ36797; RCIX1545.
DR GeneID; 5142946; -.
DR KEGG; rci:RCIX1545; -.
DR PATRIC; fig|351160.9.peg.1477; -.
DR eggNOG; arCOG00030; Archaea.
DR OMA; FIHPISD; -.
DR OrthoDB; 88302at2157; -.
DR UniPathway; UPA00591; UER00648.
DR Proteomes; UP000000663; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0052657; F:guanine phosphoribosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0004422; F:hypoxanthine phosphoribosyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0043103; P:hypoxanthine salvage; IEA:UniProtKB-UniRule.
DR GO; GO:0032264; P:IMP salvage; IEA:UniProtKB-UniPathway.
DR GO; GO:0006166; P:purine ribonucleoside salvage; IEA:UniProtKB-KW.
DR CDD; cd06223; PRTases_typeI; 1.
DR Gene3D; 3.40.50.2020; -; 1.
DR HAMAP; MF_01467; Hypx_phosphoribosyltr; 1.
DR InterPro; IPR026597; HGPRTase-like.
DR InterPro; IPR000836; PRibTrfase_dom.
DR InterPro; IPR029057; PRTase-like.
DR Pfam; PF00156; Pribosyltran; 1.
DR SUPFAM; SSF53271; SSF53271; 1.
DR PROSITE; PS00103; PUR_PYR_PR_TRANSFER; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Glycosyltransferase; Purine salvage; Reference proteome;
KW Transferase.
FT CHAIN 1..191
FT /note="Hypoxanthine/guanine phosphoribosyltransferase"
FT /id="PRO_0000415491"
SQ SEQUENCE 191 AA; 20732 MW; 553959D65ABA508B CRC64;
MLKILKESLK TAPIVKRGTY NYFIHPISDG VPVVKPELLR DVIACIVKNA DLDVDKIVTI
EAMGLPIGAS LSQITDIPFI IIRKRKYELP GEIAVHQATG YSKGELYLNG ICKGDRVLIV
DDVISTGGTL AAVIKALEMA GAEIKDIVVV IQRGNGKKII EDMGYNVQTL VTIDVDENGV
QVLGCIDDEC K