ID   AP1G2_ARATH             Reviewed;         862 AA.
AC   Q9ZUI6; Q0WSK2;
DT   05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT   05-OCT-2010, sequence version 2.
DT   03-AUG-2022, entry version 129.
DE   RecName: Full=AP-1 complex subunit gamma-2;
DE   AltName: Full=Adaptor protein complex AP-1 large subunit gamma-2;
DE   AltName: Full=Adaptor-related protein complex 1 subunit gamma-2;
DE   AltName: Full=Clathrin assembly protein complex 1 gamma-2 large chain;
DE   AltName: Full=Gamma-adaptin 2;
GN   OrderedLocusNames=At1g60070; ORFNames=T2K10.12;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA   Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   GENE FAMILY, AND REVIEW.
RX   PubMed=11598180; DOI=10.1091/mbc.12.10.2907;
RA   Boehm M., Bonifacino J.S.;
RT   "Adaptins: the final recount.";
RL   Mol. Biol. Cell 12:2907-2920(2001).
RN   [5]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND COMPONENT OF THE AP-1 COMPLEX.
RX   PubMed=23543752; DOI=10.1093/pcp/pct048;
RA   Teh O.K., Shimono Y., Shirakawa M., Fukao Y., Tamura K., Shimada T.,
RA   Hara-Nishimura I.;
RT   "The AP-1 mu adaptin is required for KNOLLE localization at the cell plate
RT   to mediate cytokinesis in Arabidopsis.";
RL   Plant Cell Physiol. 54:838-847(2013).
CC   -!- FUNCTION: Subunit of clathrin-associated adaptor protein complex 1 that
CC       plays a role in protein sorting at the trans-Golgi network and early
CC       endosomes (TGN/EE). The AP complexes mediate both the recruitment of
CC       clathrin to membranes and the recognition of sorting signals within the
CC       cytosolic tails of transmembrane cargo molecules (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Adaptor protein complex 1 (AP-1) is a heterotetramer composed
CC       of two large adaptins (gamma-type subunit and beta-type subunit), a
CC       medium adaptin (mu-type subunit) and a small adaptin (sigma-type
CC       subunit).
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus {ECO:0000250}. Cytoplasmic
CC       vesicle, clathrin-coated vesicle membrane {ECO:0000250}; Peripheral
CC       membrane protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=1;
CC         Comment=A number of isoforms are produced. According to EST
CC         sequences.;
CC       Name=1;
CC         IsoId=Q9ZUI6-1; Sequence=Displayed;
CC   -!- SIMILARITY: Belongs to the adaptor complexes large subunit family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAD14483.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AC005966; AAD14483.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002684; AEE33655.1; -; Genomic_DNA.
DR   EMBL; AK227926; BAE99896.1; -; mRNA.
DR   PIR; B96625; B96625.
DR   RefSeq; NP_176215.2; NM_104699.3. [Q9ZUI6-1]
DR   AlphaFoldDB; Q9ZUI6; -.
DR   SMR; Q9ZUI6; -.
DR   BioGRID; 27526; 6.
DR   STRING; 3702.AT1G60070.2; -.
DR   iPTMnet; Q9ZUI6; -.
DR   PaxDb; Q9ZUI6; -.
DR   PRIDE; Q9ZUI6; -.
DR   ProteomicsDB; 244961; -. [Q9ZUI6-1]
DR   EnsemblPlants; AT1G60070.1; AT1G60070.1; AT1G60070. [Q9ZUI6-1]
DR   GeneID; 842301; -.
DR   Gramene; AT1G60070.1; AT1G60070.1; AT1G60070. [Q9ZUI6-1]
DR   KEGG; ath:AT1G60070; -.
DR   Araport; AT1G60070; -.
DR   eggNOG; KOG1062; Eukaryota.
DR   HOGENOM; CLU_003824_0_0_1; -.
DR   InParanoid; Q9ZUI6; -.
DR   OMA; NEFKPVM; -.
DR   PhylomeDB; Q9ZUI6; -.
DR   PRO; PR:Q9ZUI6; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; Q9ZUI6; baseline and differential.
DR   Genevisible; Q9ZUI6; AT.
DR   GO; GO:0030121; C:AP-1 adaptor complex; IBA:GO_Central.
DR   GO; GO:0140312; F:cargo adaptor activity; IBA:GO_Central.
DR   GO; GO:0035615; F:clathrin adaptor activity; IBA:GO_Central.
DR   GO; GO:0006896; P:Golgi to vacuole transport; IBA:GO_Central.
DR   GO; GO:0006886; P:intracellular protein transport; IEA:InterPro.
DR   GO; GO:0006898; P:receptor-mediated endocytosis; IBA:GO_Central.
DR   Gene3D; 1.25.10.10; -; 1.
DR   InterPro; IPR017107; AP1_complex_gsu.
DR   InterPro; IPR011989; ARM-like.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR002553; Clathrin/coatomer_adapt-like_N.
DR   InterPro; IPR008152; Clathrin_a/b/g-adaptin_app_Ig.
DR   InterPro; IPR013041; Clathrin_app_Ig-like_sf.
DR   InterPro; IPR008153; GAE_dom.
DR   Pfam; PF01602; Adaptin_N; 1.
DR   Pfam; PF02883; Alpha_adaptinC2; 1.
DR   PIRSF; PIRSF037094; AP1_complex_gamma; 1.
DR   SMART; SM00809; Alpha_adaptinC2; 1.
DR   SUPFAM; SSF48371; SSF48371; 1.
DR   SUPFAM; SSF49348; SSF49348; 1.
DR   PROSITE; PS50180; GAE; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Cytoplasmic vesicle; Golgi apparatus; Membrane;
KW   Protein transport; Reference proteome; Repeat; Transport.
FT   CHAIN           1..862
FT                   /note="AP-1 complex subunit gamma-2"
FT                   /id="PRO_0000397853"
FT   REPEAT          1..28
FT                   /note="HEAT 1"
FT   REPEAT          29..65
FT                   /note="HEAT 2"
FT   REPEAT          101..136
FT                   /note="HEAT 3"
FT   REPEAT          137..173
FT                   /note="HEAT 4"
FT   REPEAT          308..345
FT                   /note="HEAT 5"
FT   REPEAT          346..382
FT                   /note="HEAT 6"
FT   REPEAT          384..417
FT                   /note="HEAT 7"
FT   REPEAT          418..454
FT                   /note="HEAT 8"
FT   REPEAT          458..496
FT                   /note="HEAT 9"
FT   REPEAT          507..545
FT                   /note="HEAT 10"
FT   REPEAT          560..599
FT                   /note="HEAT 11"
FT   DOMAIN          744..859
FT                   /note="GAE"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00093"
FT   CONFLICT        227
FT                   /note="L -> P (in Ref. 3; BAE99896)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   862 AA;  94670 MW;  F387B1A88C3C5CF0 CRC64;
     MNPFSSGTRL SDMIRAIRAS KTAAEERAVV RKECAAIRAS INENDQDYRH RDLAKLMFIH
     MLGYPTHFGQ MECLKLIASP GFPEKRIGYL GLMLLLDERQ EVLMLVTNSL KQDLNHTNQY
     IVGLALCALG NICSAEMARD LAPEVERLLQ FRDPNIRKKA ALCAIRIIRK VPDLSENFIN
     PGAALLKEKH HGVLITGVHL CTEICKVSSE ALEYFRKKCT EGLVKTLRDI ANSPYSPEYD
     VAGITDPFLH IRLLKLLRVL GQGDADASDC MNDILAQVAS KTESNKNAGN AILYECVQTI
     MSIEENGGLR VLAINILGKF LSNRDNNIRY VALNMLMRSL TVDSQAVQRH RATILECVKD
     SDASIQKRAL ELIYLLVNEN NVKPLAKELI EYLEVSEQDF KGDLTAKICS IVEKFAPEKI
     WYIDQMLKVL SEAGTYVKED VWHALIVVIT NAPDLHGYTV RALYRALHTS FEQETLVRVA
     IWCIGEYADL LVNNAGMLDL EDPITVTESD AVDVVENAIK HHLSDVTTKA MALIALLKIS
     SRFPSCSERV KSIIGQNKGS FVLELQQRSL EFSSVIQKHQ NIRSSLVERM PVLDEATFSG
     RRAGSLPASV STSGKSPLGI PNGVAKAAAP LVDLLDLGSD DTPAPTSSSN NFLQDLLGVD
     LSQPSAQPGA MQPSQAGADI LMDLLSIGTP APVQNGSANG DLLSIQDNNA PIAPSLTSPT
     APSSMMDLLD GFGPTPPKSE DKSAAYPSIV AFESSSLKIE FNFTKQSENP QTTDIVANFI
     NLTPNVYTEF LFQAAVPKFL QLHLDPASSN SLPANGNIKQ TMRVTNSQKG KKPIVMRMRV
     GYKINGKDVL EEGQINNFPR GL