HPSE1_ARATH
ID HPSE1_ARATH Reviewed; 543 AA.
AC Q9FF10; Q9SDA1;
DT 11-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Heparanase-like protein 1;
DE EC=3.2.-.-;
DE Flags: Precursor;
GN OrderedLocusNames=At5g07830; ORFNames=F13G24.30;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9330910; DOI=10.1093/dnares/4.3.215;
RA Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence
RT features of the 1.6 Mb regions covered by twenty physically assigned P1
RT clones.";
RL DNA Res. 4:215-230(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Endoglycosidase which is a cell surface and extracellular
CC matrix-degrading enzyme. Cleaves heparan sulfate proteoglycans (HSPGs)
CC into heparan sulfate side chains and core proteoglycans (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Lysosome membrane {ECO:0000250}; Peripheral
CC membrane protein {ECO:0000250}. Secreted {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 79 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB62595.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AB005249; BAB09947.1; -; Genomic_DNA.
DR EMBL; AL133421; CAB62595.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002688; AED91209.1; -; Genomic_DNA.
DR EMBL; BT015345; AAU05468.1; -; mRNA.
DR EMBL; BT015800; AAU93572.1; -; mRNA.
DR PIR; T45608; T45608.
DR RefSeq; NP_196400.2; NM_120865.5.
DR AlphaFoldDB; Q9FF10; -.
DR SMR; Q9FF10; -.
DR BioGRID; 15954; 1.
DR STRING; 3702.AT5G07830.1; -.
DR CAZy; GH79; Glycoside Hydrolase Family 79.
DR PaxDb; Q9FF10; -.
DR PRIDE; Q9FF10; -.
DR ProteomicsDB; 230268; -.
DR EnsemblPlants; AT5G07830.1; AT5G07830.1; AT5G07830.
DR GeneID; 830676; -.
DR Gramene; AT5G07830.1; AT5G07830.1; AT5G07830.
DR KEGG; ath:AT5G07830; -.
DR Araport; AT5G07830; -.
DR TAIR; locus:2142768; AT5G07830.
DR eggNOG; ENOG502QQST; Eukaryota.
DR HOGENOM; CLU_021823_3_0_1; -.
DR InParanoid; Q9FF10; -.
DR OMA; SGFMWLD; -.
DR OrthoDB; 1132327at2759; -.
DR PhylomeDB; Q9FF10; -.
DR BioCyc; ARA:AT5G07830-MON; -.
DR PRO; PR:Q9FF10; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FF10; baseline and differential.
DR Genevisible; Q9FF10; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009505; C:plant-type cell wall; HDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0004566; F:beta-glucuronidase activity; IDA:TAIR.
DR GO; GO:0009826; P:unidimensional cell growth; IMP:TAIR.
DR InterPro; IPR005199; Glyco_hydro_79.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR Pfam; PF03662; Glyco_hydro_79n; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Hydrolase; Lysosome; Membrane; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..543
FT /note="Heparanase-like protein 1"
FT /id="PRO_0000042269"
FT ACT_SITE 201
FT /note="Proton donor"
FT /evidence="ECO:0000255"
FT ACT_SITE 320
FT /note="Nucleophile"
FT /evidence="ECO:0000255"
FT CARBOHYD 184
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 304
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 425
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 428
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 543 AA; 60250 MW; 0FA2248948282FF6 CRC64;
MGFRVCVIVV FLGCLLLVPE KTMAQEMKRA SIVIQGARRV CETDENFVCA TLDWWPHDKC
NYDQCPWGYS SVINMDLTRP LLTKAIKAFK PLRIRIGGSL QDQVIYDVGN LKTPCRPFQK
MNSGLFGFSK GCLHMKRWDE LNSFLTATGA VVTFGLNALR GRHKLRGKAW GGAWDHINTQ
DFLNYTVSKG YVIDSWEFGN ELSGSGVGAS VSAELYGKDL IVLKDVINKV YKNSWLHKPI
LVAPGGFYEQ QWYTKLLEIS GPSVVDVVTH HIYNLGSGND PALVKKIMDP SYLSQVSKTF
KDVNQTIQEH GPWASPWVGE SGGAYNSGGR HVSDTFIDSF WYLDQLGMSA RHNTKVYCRQ
TLVGGFYGLL EKGTFVPNPD YYSALLWHRL MGKGVLAVQT DGPPQLRVYA HCSKGRAGVT
LLLINLSNQS DFTVSVSNGI NVVLNAESRK KKSLLDTLKR PFSWIGSKAS DGYLNREEYH
LTPENGVLRS KTMVLNGKSL KPTATGDIPS LEPVLRSVNS PLNVLPLSMS FIVLPNFDAS
ACS
