ID   HPSE2_ARATH             Reviewed;         539 AA.
AC   Q8L608; Q9FLK8;
DT   11-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=Heparanase-like protein 2;
DE            EC=3.2.-.-;
DE   Flags: Precursor;
GN   OrderedLocusNames=At5g61250; ORFNames=MAF19.29, MFB13.2;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9628582; DOI=10.1093/dnares/5.1.41;
RA   Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence
RT   features of the regions of 1,456,315 bp covered by nineteen physically
RT   assigned P1 and TAC clones.";
RL   DNA Res. 5:41-54(1998).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
CC   -!- FUNCTION: Endoglycosidase which is a cell surface and extracellular
CC       matrix-degrading enzyme. Cleaves heparan sulfate proteoglycans (HSPGs)
CC       into heparan sulfate side chains and core proteoglycans (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Lysosome membrane {ECO:0000250}; Peripheral
CC       membrane protein {ECO:0000250}. Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 79 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAB08480.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AB010073; BAB08480.1; ALT_INIT; Genomic_DNA.
DR   EMBL; CP002688; AED97442.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED97443.1; -; Genomic_DNA.
DR   EMBL; CP002688; ANM68404.1; -; Genomic_DNA.
DR   EMBL; AY099736; AAM20587.1; -; mRNA.
DR   EMBL; BT002603; AAO00963.1; -; mRNA.
DR   RefSeq; NP_001330163.1; NM_001345460.1.
DR   RefSeq; NP_200933.2; NM_125518.5.
DR   RefSeq; NP_851238.1; NM_180907.3.
DR   AlphaFoldDB; Q8L608; -.
DR   SMR; Q8L608; -.
DR   STRING; 3702.AT5G61250.2; -.
DR   CAZy; GH79; Glycoside Hydrolase Family 79.
DR   PaxDb; Q8L608; -.
DR   PRIDE; Q8L608; -.
DR   ProteomicsDB; 230139; -.
DR   EnsemblPlants; AT5G61250.1; AT5G61250.1; AT5G61250.
DR   EnsemblPlants; AT5G61250.2; AT5G61250.2; AT5G61250.
DR   EnsemblPlants; AT5G61250.3; AT5G61250.3; AT5G61250.
DR   GeneID; 836246; -.
DR   Gramene; AT5G61250.1; AT5G61250.1; AT5G61250.
DR   Gramene; AT5G61250.2; AT5G61250.2; AT5G61250.
DR   Gramene; AT5G61250.3; AT5G61250.3; AT5G61250.
DR   KEGG; ath:AT5G61250; -.
DR   Araport; AT5G61250; -.
DR   TAIR; locus:2163243; AT5G61250.
DR   eggNOG; ENOG502QQST; Eukaryota.
DR   HOGENOM; CLU_021823_3_0_1; -.
DR   InParanoid; Q8L608; -.
DR   OMA; STKVAWT; -.
DR   OrthoDB; 1132327at2759; -.
DR   PhylomeDB; Q8L608; -.
DR   PRO; PR:Q8L608; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q8L608; baseline and differential.
DR   Genevisible; Q8L608; AT.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0004566; F:beta-glucuronidase activity; ISS:TAIR.
DR   InterPro; IPR005199; Glyco_hydro_79.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   Pfam; PF03662; Glyco_hydro_79n; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Hydrolase; Lysosome; Membrane; Reference proteome; Secreted;
KW   Signal.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..539
FT                   /note="Heparanase-like protein 2"
FT                   /id="PRO_0000042270"
FT   ACT_SITE        198
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        316
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        143
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        163
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        181
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        300
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        421
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   539 AA;  59865 MW;  A6D4B3D4A3627DA2 CRC64;
     MGFNVVVFLS CLLLLPPVTF GSNMERTTLV IDGSRRIAET DENFICATLD WWPPEKCNYD
     QCPWGYASLI NLNLASPLLA KAIQAFRTLR IRIGGSLQDQ VIYDVGDLKT PCTQFKKTDD
     GLFGFSEGCL YMKRWDEVNH FFNATGAIVT FGLNALHGRN KLNGTAWGGD WDHTNTQDFM
     NYTVSKGYAI DSWEFGNELS GSGIWASVSV ELYGKDLIVL KNVIKNVYKN SRTKPLVVAP
     GGFFEEQWYS ELLRLSGPGV LDVLTHHIYN LGPGNDPKLV NKILDPNYLS GISELFANVN
     QTIQEHGPWA AAWVGEAGGA FNSGGRQVSE TFINSFWYLD QLGISSKHNT KVYCRQALVG
     GFYGLLEKET FVPNPDYYSA LLWHRLMGKG ILGVQTTASE YLRAYVHCSK RRAGITILLI
     NLSKHTTFTV AVSNGVKVVL QAESMKRKSF LETIKSKVSW VGNKASDGYL NREEYHLSPK
     DGDLRSKIML LNGKPLVPTA TGDIPKLEPV RHGVKSPVYI NPLSISFIVL PTFDAPACS