ID   HPSE2_MOUSE             Reviewed;         592 AA.
AC   B2RY83;
DT   05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-2008, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Inactive heparanase-2;
DE            Short=Hpa2;
DE   Flags: Precursor;
GN   Name=Hpse2; Synonyms=Hpa2;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Binds heparin and heparan sulfate with high affinity, but
CC       lacks heparanase activity. Inhibits HPSE, possibly by competing for its
CC       substrates (in vitro) (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with HPSE. Interacts with SDC1 (via glycan chains)
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular
CC       matrix {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 79 family. {ECO:0000305}.
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DR   EMBL; AC122312; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AC124716; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AC125458; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AC126795; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AC140375; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC158131; AAI58132.1; -; mRNA.
DR   EMBL; BC158133; AAI58134.1; -; mRNA.
DR   EMBL; BC158136; AAI58137.1; -; mRNA.
DR   CCDS; CCDS37993.1; -.
DR   RefSeq; NP_001074726.1; NM_001081257.2.
DR   AlphaFoldDB; B2RY83; -.
DR   SMR; B2RY83; -.
DR   STRING; 10090.ENSMUSP00000097026; -.
DR   CAZy; GH79; Glycoside Hydrolase Family 79.
DR   GlyGen; B2RY83; 2 sites.
DR   iPTMnet; B2RY83; -.
DR   PhosphoSitePlus; B2RY83; -.
DR   PaxDb; B2RY83; -.
DR   PRIDE; B2RY83; -.
DR   ProteomicsDB; 273273; -.
DR   Antibodypedia; 45898; 185 antibodies from 29 providers.
DR   Ensembl; ENSMUST00000099428; ENSMUSP00000097026; ENSMUSG00000074852.
DR   GeneID; 545291; -.
DR   KEGG; mmu:545291; -.
DR   UCSC; uc008hoe.2; mouse.
DR   CTD; 60495; -.
DR   MGI; MGI:2685814; Hpse2.
DR   VEuPathDB; HostDB:ENSMUSG00000074852; -.
DR   eggNOG; ENOG502QQST; Eukaryota.
DR   GeneTree; ENSGT00390000004874; -.
DR   HOGENOM; CLU_021823_0_1_1; -.
DR   InParanoid; B2RY83; -.
DR   OMA; YSRAHLY; -.
DR   OrthoDB; 1132327at2759; -.
DR   PhylomeDB; B2RY83; -.
DR   TreeFam; TF328999; -.
DR   Reactome; R-MMU-2024096; HS-GAG degradation.
DR   BioGRID-ORCS; 545291; 0 hits in 70 CRISPR screens.
DR   ChiTaRS; Hpse2; mouse.
DR   PRO; PR:B2RY83; -.
DR   Proteomes; UP000000589; Chromosome 19.
DR   RNAct; B2RY83; protein.
DR   Bgee; ENSMUSG00000074852; Expressed in undifferentiated genital tubercle and 41 other tissues.
DR   GO; GO:0031012; C:extracellular matrix; ISO:MGI.
DR   GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR   GO; GO:0016020; C:membrane; IEA:InterPro.
DR   GO; GO:0043395; F:heparan sulfate proteoglycan binding; ISO:MGI.
DR   GO; GO:0016798; F:hydrolase activity, acting on glycosyl bonds; IEA:InterPro.
DR   GO; GO:0008283; P:cell population proliferation; IMP:MGI.
DR   GO; GO:0030198; P:extracellular matrix organization; IMP:MGI.
DR   GO; GO:0008284; P:positive regulation of cell population proliferation; IMP:MGI.
DR   InterPro; IPR005199; Glyco_hydro_79.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   Pfam; PF03662; Glyco_hydro_79n; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
PE   2: Evidence at transcript level;
KW   Extracellular matrix; Glycoprotein; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..38
FT                   /evidence="ECO:0000255"
FT   CHAIN           39..592
FT                   /note="Inactive heparanase-2"
FT                   /id="PRO_0000418888"
FT   CARBOHYD        254
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        392
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   592 AA;  66685 MW;  C82F7A502B776E1F CRC64;
     MRVLCAFPEA MASSSSRPPS CLALVALFLA LLLHLSLSFH AGNRRPLPVD RATGLKEKTL
     ILLDVSTKNP VRTVNENFLS LQLDPSIIHD GWLDFLSSKR LVTLARGLSP AFLRFGGKRT
     DFLQFQNLRN PAKSRGGPGP DYYLKNYEDD IVRSDVALDK QKGCKIAQHP DVMLELQREK
     ASQMHLVLLK EQYSNTYSNL ILTARSLDKL YNFADCSGLH LIFALNALRR NPNNSWNSSS
     ALSLLKYSAS KKYNISWELG NEPNNYRSIH GRAVNGSQLG KDYIQLKSLL QPIRVYSRAS
     LYGPNIGRPR KNVIALLDGF MKVAGSTVDA VTWQHCYIDG RVVKVMDFLK TRLLDTLSDQ
     IRKIQKVVNT YTPGKKIWLE GVVTTSAGGT NNLSDSYAAG FLWLNTLGML ANQGIDVVIR
     HSFFDHGYNH LVDQNFNPLP DYWLSLLYKR LIGPKVLAVH VAGLQRKPRP GRVIRDKLRI
     YAHCTNHHNH NYVRGSITLF IINLHRSRKK IKLAGTLRDK LVHQYLLQPY GQEGLKSKSV
     QLNGQPLVMV DDGTLPELKP RPLRAGRTLV IPPVTMGFYV VKNVNALACR YR