ID   HPS_METAM               Reviewed;         209 AA.
AC   Q48907;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=3-hexulose-6-phosphate synthase;
DE            Short=HPS;
DE            EC=4.1.2.43;
DE   AltName: Full=D-arabino-3-hexulose-6-phosphate formaldehyde lyase;
GN   Name=rmpA; Synonyms=hps;
OS   Methylomonas aminofaciens.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Methylococcales;
OC   Methylococcaceae; Methylomonas.
OX   NCBI_TaxID=46896;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-48; 64-77; 103-107
RP   AND 179-198, AND SUBUNIT.
RC   STRAIN=77a;
RX   PubMed=8595859; DOI=10.1111/j.1574-6968.1996.tb07990.x;
RA   Yanase H., Ikeyama K., Mitsui R., Ra S., Kita K., Sakai Y., Kato N.;
RT   "Cloning and sequence analysis of the gene encoding 3-hexulose-6-phosphate
RT   synthase from the methylotrophic bacterium, Methylomonas aminofaciens 77a,
RT   and its expression in Escherichia coli.";
RL   FEMS Microbiol. Lett. 135:201-205(1996).
RN   [2]
RP   FUNCTION.
RC   STRAIN=77a;
RX   PubMed=10418139; DOI=10.1111/j.1574-6968.1999.tb13652.x;
RA   Sakai Y., Mitsui R., Katayama Y., Yanase H., Kato N.;
RT   "Organization of the genes involved in the ribulose monophosphate pathway
RT   in an obligate methylotrophic bacterium, Methylomonas aminofaciens 77a.";
RL   FEMS Microbiol. Lett. 176:125-130(1999).
CC   -!- FUNCTION: Catalyzes the condensation of ribulose 5-phosphate with
CC       formaldehyde to form 3-hexulose 6-phosphate.
CC       {ECO:0000269|PubMed:10418139}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-ribulose 5-phosphate + formaldehyde = D-arabino-hex-3-ulose
CC         6-phosphate; Xref=Rhea:RHEA:25201, ChEBI:CHEBI:16842,
CC         ChEBI:CHEBI:58121, ChEBI:CHEBI:58542; EC=4.1.2.43;
CC   -!- PATHWAY: One-carbon metabolism; formaldehyde assimilation via RuMP
CC       pathway; D-fructose 6-phosphate from D-ribulose 5-phosphate and
CC       formaldehyde: step 1/2.
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:8595859}.
CC   -!- SIMILARITY: Belongs to the HPS/KGPDC family. HPS subfamily.
CC       {ECO:0000305}.
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DR   EMBL; D64136; BAA19967.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q48907; -.
DR   SMR; Q48907; -.
DR   KEGG; ag:BAA19967; -.
DR   BRENDA; 4.1.2.43; 7933.
DR   UniPathway; UPA00294; UER00434.
DR   GO; GO:0043801; F:hexulose-6-phosphate synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0004590; F:orotidine-5'-phosphate decarboxylase activity; IEA:InterPro.
DR   GO; GO:0006207; P:'de novo' pyrimidine nucleobase biosynthetic process; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0019647; P:formaldehyde assimilation via ribulose monophosphate cycle; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW.
DR   CDD; cd04726; KGPDC_HPS; 1.
DR   Gene3D; 3.20.20.70; -; 1.
DR   InterPro; IPR017553; 3-hexulose-6-phosphate_synth.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR041710; HPS/KGPDC.
DR   InterPro; IPR001754; OMPdeCOase_dom.
DR   InterPro; IPR011060; RibuloseP-bd_barrel.
DR   Pfam; PF00215; OMPdecase; 1.
DR   SMART; SM00934; OMPdecase; 1.
DR   SUPFAM; SSF51366; SSF51366; 1.
DR   TIGRFAMs; TIGR03128; RuMP_HxlA; 1.
PE   1: Evidence at protein level;
KW   Carbohydrate metabolism; Direct protein sequencing; Lyase;
KW   One-carbon metabolism.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:8595859"
FT   CHAIN           2..209
FT                   /note="3-hexulose-6-phosphate synthase"
FT                   /id="PRO_0000212102"
SQ   SEQUENCE   209 AA;  21357 MW;  A5FA39433F16FF1C CRC64;
     MALTQMALDS LDFDATVALA EKVAPHVDIL EIGTPCIKHN GIKLLETLRA KFPNNKILVD
     LKTMDAGFYE AEPFYKAGAD ITTVLGVADL GTIKGVIDAA NKYGKKAQID LINVGDKAAR
     TKEVAKLGAH IIGVHTGLDQ QAAGQTPFAD LATVTGLNLG LEVSVAGGVK PATVAQVKDA
     GATIIVAGAA IYGAADPAAA AAEITGLAK