HPS_MYCGS
ID HPS_MYCGS Reviewed; 207 AA.
AC Q9LBW4;
DT 16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 65.
DE RecName: Full=3-hexulose-6-phosphate synthase;
DE Short=HPS;
DE EC=4.1.2.43;
DE AltName: Full=D-arabino-3-hexulose-6-phosphate formaldehyde lyase;
GN Name=rmpA;
OS Mycobacterium gastri.
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium.
OX NCBI_TaxID=1777;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND TRANSCRIPTIONAL
RP REGULATION.
RC STRAIN=MB19;
RX PubMed=10648518; DOI=10.1128/jb.182.4.944-948.2000;
RA Mitsui R., Sakai Y., Yasueda H., Kato N.;
RT "A novel operon encoding formaldehyde fixation: the ribulose monophosphate
RT pathway in the Gram-positive facultative methylotrophic bacterium
RT Mycobacterium gastri MB19.";
RL J. Bacteriol. 182:944-948(2000).
CC -!- FUNCTION: Catalyzes the condensation of ribulose 5-phosphate with
CC formaldehyde to form 3-hexulose 6-phosphate.
CC {ECO:0000269|PubMed:10648518}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-ribulose 5-phosphate + formaldehyde = D-arabino-hex-3-ulose
CC 6-phosphate; Xref=Rhea:RHEA:25201, ChEBI:CHEBI:16842,
CC ChEBI:CHEBI:58121, ChEBI:CHEBI:58542; EC=4.1.2.43;
CC -!- PATHWAY: One-carbon metabolism; formaldehyde assimilation via RuMP
CC pathway; D-fructose 6-phosphate from D-ribulose 5-phosphate and
CC formaldehyde: step 1/2.
CC -!- INDUCTION: By methanol or methylamine. {ECO:0000269|PubMed:10648518}.
CC -!- SIMILARITY: Belongs to the HPS/KGPDC family. HPS subfamily.
CC {ECO:0000305}.
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DR EMBL; AB034913; BAA90546.1; -; Genomic_DNA.
DR PDB; 3AJX; X-ray; 1.60 A; A/B/C/D=1-207.
DR PDBsum; 3AJX; -.
DR AlphaFoldDB; Q9LBW4; -.
DR SMR; Q9LBW4; -.
DR BRENDA; 4.1.2.43; 10297.
DR UniPathway; UPA00294; UER00434.
DR EvolutionaryTrace; Q9LBW4; -.
DR GO; GO:0043801; F:hexulose-6-phosphate synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0004590; F:orotidine-5'-phosphate decarboxylase activity; IEA:InterPro.
DR GO; GO:0006207; P:'de novo' pyrimidine nucleobase biosynthetic process; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0019647; P:formaldehyde assimilation via ribulose monophosphate cycle; IEA:UniProtKB-UniPathway.
DR GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW.
DR CDD; cd04726; KGPDC_HPS; 1.
DR Gene3D; 3.20.20.70; -; 1.
DR InterPro; IPR017553; 3-hexulose-6-phosphate_synth.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR041710; HPS/KGPDC.
DR InterPro; IPR001754; OMPdeCOase_dom.
DR InterPro; IPR011060; RibuloseP-bd_barrel.
DR Pfam; PF00215; OMPdecase; 1.
DR SMART; SM00934; OMPdecase; 1.
DR SUPFAM; SSF51366; SSF51366; 1.
DR TIGRFAMs; TIGR03128; RuMP_HxlA; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Carbohydrate metabolism; Lyase; One-carbon metabolism.
FT CHAIN 1..207
FT /note="3-hexulose-6-phosphate synthase"
FT /id="PRO_0000235167"
FT STRAND 2..7
FT /evidence="ECO:0007829|PDB:3AJX"
FT HELIX 12..22
FT /evidence="ECO:0007829|PDB:3AJX"
FT HELIX 23..25
FT /evidence="ECO:0007829|PDB:3AJX"
FT STRAND 27..31
FT /evidence="ECO:0007829|PDB:3AJX"
FT HELIX 33..39
FT /evidence="ECO:0007829|PDB:3AJX"
FT HELIX 42..50
FT /evidence="ECO:0007829|PDB:3AJX"
FT STRAND 54..62
FT /evidence="ECO:0007829|PDB:3AJX"
FT HELIX 66..75
FT /evidence="ECO:0007829|PDB:3AJX"
FT STRAND 79..84
FT /evidence="ECO:0007829|PDB:3AJX"
FT HELIX 89..102
FT /evidence="ECO:0007829|PDB:3AJX"
FT STRAND 105..109
FT /evidence="ECO:0007829|PDB:3AJX"
FT HELIX 116..125
FT /evidence="ECO:0007829|PDB:3AJX"
FT STRAND 129..134
FT /evidence="ECO:0007829|PDB:3AJX"
FT HELIX 137..140
FT /evidence="ECO:0007829|PDB:3AJX"
FT HELIX 148..157
FT /evidence="ECO:0007829|PDB:3AJX"
FT STRAND 161..166
FT /evidence="ECO:0007829|PDB:3AJX"
FT HELIX 169..171
FT /evidence="ECO:0007829|PDB:3AJX"
FT HELIX 172..177
FT /evidence="ECO:0007829|PDB:3AJX"
FT STRAND 181..186
FT /evidence="ECO:0007829|PDB:3AJX"
FT HELIX 187..190
FT /evidence="ECO:0007829|PDB:3AJX"
FT STRAND 192..194
FT /evidence="ECO:0007829|PDB:3AJX"
FT HELIX 195..204
FT /evidence="ECO:0007829|PDB:3AJX"
SQ SEQUENCE 207 AA; 20936 MW; 50A77E4688F937D2 CRC64;
MKLQVAIDLL STEAALELAG KVAEYVDIIE LGTPLIEAEG LSVITAVKKA HPDKIVFADM
KTMDAGELEA DIAFKAGADL VTVLGSADDS TIAGAVKAAQ AHNKGVVVDL IGIEDKATRA
QEVRALGAKF VEMHAGLDEQ AKPGFDLNGL LAAGEKARVP FSVAGGVKVA TIPAVQKAGA
EVAVAGGAIY GAADPAAAAK ELRAAIA
