ID   HPS_STAAS               Reviewed;         210 AA.
AC   Q6GBR7;
DT   12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=3-hexulose-6-phosphate synthase;
DE            Short=HPS;
DE            EC=4.1.2.43;
DE   AltName: Full=D-arabino-3-hexulose-6-phosphate formaldehyde lyase;
GN   OrderedLocusNames=SAS0528;
OS   Staphylococcus aureus (strain MSSA476).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=282459;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MSSA476;
RX   PubMed=15213324; DOI=10.1073/pnas.0402521101;
RA   Holden M.T.G., Feil E.J., Lindsay J.A., Peacock S.J., Day N.P.J.,
RA   Enright M.C., Foster T.J., Moore C.E., Hurst L., Atkin R., Barron A.,
RA   Bason N., Bentley S.D., Chillingworth C., Chillingworth T., Churcher C.,
RA   Clark L., Corton C., Cronin A., Doggett J., Dowd L., Feltwell T., Hance Z.,
RA   Harris B., Hauser H., Holroyd S., Jagels K., James K.D., Lennard N.,
RA   Line A., Mayes R., Moule S., Mungall K., Ormond D., Quail M.A.,
RA   Rabbinowitsch E., Rutherford K.M., Sanders M., Sharp S., Simmonds M.,
RA   Stevens K., Whitehead S., Barrell B.G., Spratt B.G., Parkhill J.;
RT   "Complete genomes of two clinical Staphylococcus aureus strains: evidence
RT   for the rapid evolution of virulence and drug resistance.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:9786-9791(2004).
CC   -!- FUNCTION: Catalyzes the condensation of ribulose 5-phosphate with
CC       formaldehyde to form 3-hexulose 6-phosphate. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-ribulose 5-phosphate + formaldehyde = D-arabino-hex-3-ulose
CC         6-phosphate; Xref=Rhea:RHEA:25201, ChEBI:CHEBI:16842,
CC         ChEBI:CHEBI:58121, ChEBI:CHEBI:58542; EC=4.1.2.43;
CC   -!- PATHWAY: One-carbon metabolism; formaldehyde assimilation via RuMP
CC       pathway; D-fructose 6-phosphate from D-ribulose 5-phosphate and
CC       formaldehyde: step 1/2.
CC   -!- SIMILARITY: Belongs to the HPS/KGPDC family. HPS subfamily.
CC       {ECO:0000305}.
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DR   EMBL; BX571857; CAG42303.1; -; Genomic_DNA.
DR   RefSeq; WP_000422864.1; NC_002953.3.
DR   AlphaFoldDB; Q6GBR7; -.
DR   SMR; Q6GBR7; -.
DR   KEGG; sas:SAS0528; -.
DR   HOGENOM; CLU_081825_1_0_9; -.
DR   OMA; WMTVICA; -.
DR   UniPathway; UPA00294; UER00434.
DR   GO; GO:0043801; F:hexulose-6-phosphate synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0004590; F:orotidine-5'-phosphate decarboxylase activity; IEA:InterPro.
DR   GO; GO:0006207; P:'de novo' pyrimidine nucleobase biosynthetic process; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0019647; P:formaldehyde assimilation via ribulose monophosphate cycle; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW.
DR   CDD; cd04726; KGPDC_HPS; 1.
DR   Gene3D; 3.20.20.70; -; 1.
DR   InterPro; IPR017553; 3-hexulose-6-phosphate_synth.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR041710; HPS/KGPDC.
DR   InterPro; IPR001754; OMPdeCOase_dom.
DR   InterPro; IPR011060; RibuloseP-bd_barrel.
DR   Pfam; PF00215; OMPdecase; 1.
DR   SMART; SM00934; OMPdecase; 1.
DR   SUPFAM; SSF51366; SSF51366; 1.
DR   TIGRFAMs; TIGR03128; RuMP_HxlA; 1.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism; Lyase; One-carbon metabolism.
FT   CHAIN           1..210
FT                   /note="3-hexulose-6-phosphate synthase"
FT                   /id="PRO_0000269522"
SQ   SEQUENCE   210 AA;  22450 MW;  7C952A8081682538 CRC64;
     MELQLAIDLL NKEDAAELAN KVKDYVDIVE IGTPIIYNEG LPAVKHMADN ISNVKVLADM
     KIMDAADYEV SQAIKFGADV ITILGVAEDA SIKAAIEEAH KNNKQLLVDM IAVQDLEKRA
     KELDEMGADY IAVHTGYDLQ AEGQSPLESL RTVKSVIKNS KVAVAGGIKP DTIKEIVAES
     PDLVIVGGGI ANADDPVEAA KQCRAAIEGK