HPT2_ORYSJ
ID HPT2_ORYSJ Reviewed; 379 AA.
AC Q0D576; A0A0P0X8E9; B9FY26; Q6ZLA8;
DT 28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT 13-OCT-2009, sequence version 2.
DT 03-AUG-2022, entry version 78.
DE RecName: Full=Probable homogentisate phytyltransferase 2, chloroplastic;
DE EC=2.5.1.115;
DE AltName: Full=Vitamin E pathway gene 2-2 protein;
DE Short=OsVTE2-2;
DE Flags: Precursor;
GN Name=HPT2; Synonyms=VTE2-2; OrderedLocusNames=Os07g0576000, LOC_Os07g38850;
GN ORFNames=OJ1065_B06.2-1, OsJ_24854;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [5]
RP NOMENCLATURE.
RX PubMed=16408209; DOI=10.1007/s00425-005-0180-1;
RA Venkatesh T.V., Karunanandaa B., Free D.L., Rottnek J.M., Baszis S.R.,
RA Valentin H.E.;
RT "Identification and characterization of an Arabidopsis homogentisate
RT phytyltransferase paralog.";
RL Planta 223:1134-1144(2006).
CC -!- FUNCTION: Involved in the synthesis of tocopherol (vitamin E).
CC Catalyzes the condensation of homogentisate and phytyl diphosphate to
CC form dimethylphytylhydrquinone (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+) + homogentisate + phytyl diphosphate = 2-methyl-6-phytyl-
CC 1,4-benzene-1,4-diol + CO2 + diphosphate; Xref=Rhea:RHEA:37975,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16169, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:75434, ChEBI:CHEBI:75920;
CC EC=2.5.1.115;
CC -!- PATHWAY: Cofactor biosynthesis; tocopherol biosynthesis.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the UbiA prenyltransferase family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC83059.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AP003804; BAC83059.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008213; BAF21997.2; -; Genomic_DNA.
DR EMBL; AP014963; BAT02293.1; -; Genomic_DNA.
DR EMBL; CM000144; EEE67463.1; -; Genomic_DNA.
DR RefSeq; XP_015646905.1; XM_015791419.1.
DR AlphaFoldDB; Q0D576; -.
DR SMR; Q0D576; -.
DR STRING; 4530.OS07T0576000-02; -.
DR PRIDE; Q0D576; -.
DR EnsemblPlants; Os07t0576000-01; Os07t0576000-01; Os07g0576000.
DR GeneID; 4343702; -.
DR Gramene; Os07t0576000-01; Os07t0576000-01; Os07g0576000.
DR KEGG; osa:4343702; -.
DR eggNOG; ENOG502QUHT; Eukaryota.
DR HOGENOM; CLU_048963_2_1_1; -.
DR InParanoid; Q0D576; -.
DR OrthoDB; 1231215at2759; -.
DR UniPathway; UPA00160; -.
DR Proteomes; UP000000763; Chromosome 7.
DR Proteomes; UP000007752; Chromosome 7.
DR Proteomes; UP000059680; Chromosome 7.
DR ExpressionAtlas; Q0D576; baseline and differential.
DR Genevisible; Q0D576; OS.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0010176; F:homogentisate phytyltransferase activity; IEA:RHEA.
DR GO; GO:0010189; P:vitamin E biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd13960; PT_UbiA_HPT1; 1.
DR Gene3D; 1.10.357.140; -; 1.
DR InterPro; IPR044502; AtHST-like.
DR InterPro; IPR000537; UbiA_prenyltransferase.
DR InterPro; IPR044878; UbiA_sf.
DR Pfam; PF01040; UbiA; 1.
PE 3: Inferred from homology;
KW Chloroplast; Membrane; Plastid; Reference proteome; Thylakoid; Transferase;
KW Transit peptide; Transmembrane; Transmembrane helix.
FT TRANSIT 1..65
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 66..379
FT /note="Probable homogentisate phytyltransferase 2,
FT chloroplastic"
FT /id="PRO_0000409871"
FT TRANSMEM 121..141
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 174..194
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 195..215
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 220..240
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 252..272
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 299..319
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 328..348
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 361..378
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..39
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 379 AA; 41422 MW; 3D68C60960AE6C01 CRC64;
MASLASPPLP CRAAATASRS GRPAPRLLGP PPPPASPLLS SASARFPRAP CNAARWSRRD
AVRVCSQAGA AGPAPLSKTL SDLKDSCWRF LRPHTIRGTA LGSIALVARA LIENPQLINW
WLVFKAFYGL VALICGNGYI VGINQIYDIR IDKVNKPYLP IAAGDLSVQT AWLLVVLFAA
AGFSIVVTNF GPFITSLYCL GLFLGTIYSV PPFRLKRYPV AAFLIIATVR GFLLNFGVYY
ATRAALGLTF QWSSPVAFIT CFVTLFALVI AITKDLPDVE GDRKYQISTL ATKLGVRNIA
FLGSGLLIAN YVAAIAVAFL MPQAFRRTVM VPVHAALAVG IIFQTWVLEQ AKYTKDAISQ
YYRFIWNLFY AEYIFFPLI
