ID   HPTR_STAAR              Reviewed;         252 AA.
AC   Q6GK93;
DT   21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 112.
DE   RecName: Full=Transcriptional regulatory protein HptR;
GN   Name=hptR; OrderedLocusNames=SAR0214;
OS   Staphylococcus aureus (strain MRSA252).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=282458;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MRSA252;
RX   PubMed=15213324; DOI=10.1073/pnas.0402521101;
RA   Holden M.T.G., Feil E.J., Lindsay J.A., Peacock S.J., Day N.P.J.,
RA   Enright M.C., Foster T.J., Moore C.E., Hurst L., Atkin R., Barron A.,
RA   Bason N., Bentley S.D., Chillingworth C., Chillingworth T., Churcher C.,
RA   Clark L., Corton C., Cronin A., Doggett J., Dowd L., Feltwell T., Hance Z.,
RA   Harris B., Hauser H., Holroyd S., Jagels K., James K.D., Lennard N.,
RA   Line A., Mayes R., Moule S., Mungall K., Ormond D., Quail M.A.,
RA   Rabbinowitsch E., Rutherford K.M., Sanders M., Sharp S., Simmonds M.,
RA   Stevens K., Whitehead S., Barrell B.G., Spratt B.G., Parkhill J.;
RT   "Complete genomes of two clinical Staphylococcus aureus strains: evidence
RT   for the rapid evolution of virulence and drug resistance.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:9786-9791(2004).
CC   -!- FUNCTION: Member of the two-component regulatory system HptS/HptR that
CC       regulates genes involved in hexose phosphate transport system in
CC       response to changes in extracellular phosphate sources. Activates uhpT
CC       expression to facilitate glucose-6-phosphate/G6P utilization by
CC       directly binding to its promoter. Antagonizes CcpA-dependent
CC       transcription of a subset of CcpA-regulated genes involved in
CC       antibiotic susceptibility. {ECO:0000250|UniProtKB:Q2G1E1}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- PTM: Phosphorylated by HptS. {ECO:0000250|UniProtKB:Q2G1E1}.
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DR   EMBL; BX571856; CAG39241.1; -; Genomic_DNA.
DR   RefSeq; WP_000477517.1; NC_002952.2.
DR   AlphaFoldDB; Q6GK93; -.
DR   SMR; Q6GK93; -.
DR   KEGG; sar:SAR0214; -.
DR   HOGENOM; CLU_000445_5_1_9; -.
DR   OMA; IMTAFEM; -.
DR   OrthoDB; 1755994at2; -.
DR   Proteomes; UP000000596; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR018060; HTH_AraC.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   Pfam; PF12833; HTH_18; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   SMART; SM00342; HTH_ARAC; 1.
DR   SMART; SM00448; REC; 1.
DR   SUPFAM; SSF46689; SSF46689; 2.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   PROSITE; PS01124; HTH_ARAC_FAMILY_2; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Phosphoprotein; Transcription;
KW   Transcription regulation; Two-component regulatory system.
FT   CHAIN           1..252
FT                   /note="Transcriptional regulatory protein HptR"
FT                   /id="PRO_0000299110"
FT   DOMAIN          3..118
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   DOMAIN          153..250
FT                   /note="HTH araC/xylS-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT   DNA_BIND        170..191
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT   DNA_BIND        217..240
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT   MOD_RES         55
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ   SEQUENCE   252 AA;  29609 MW;  C72C8E41A3CE41CB CRC64;
     MFKVVICDDE RIIREGLKQI IPWGDYHFNT IYTAKDGVEA LSLIQQHQPE LVITDIRMPR
     KNGVDLLNDI AHLDCNVIIL SSYDDFEYMK AGIQHHVLDY LLKPVDHAQL EVILGRLVRT
     LLEQQSQNGR SLAPCHDAFQ PLLKVEYDDY YVNQIVDQIK QSYQTKVTVS DLIQHIDVSE
     SYAMRTFKDH VGITIVDYLN RYRILQSLQL LDRHYKHYEI ADKVGFSEYK MFSYHFKKYL
     QMSPSDYCKQ AK