ID   HR29_HALRO              Reviewed;         252 AA.
AC   Q04757;
DT   07-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   25-MAY-2022, entry version 70.
DE   RecName: Full=Body wall muscle protein HR-29;
OS   Halocynthia roretzi (Sea squirt) (Cynthia roretzi).
OC   Eukaryota; Metazoa; Chordata; Tunicata; Ascidiacea; Stolidobranchia;
OC   Pyuridae; Halocynthia.
OX   NCBI_TaxID=7729;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], PROTEIN SEQUENCE OF 2-252, AND
RP   ACETYLATION AT SER-2.
RC   TISSUE=Body wall muscle;
RX   PubMed=8486605; DOI=10.1093/oxfordjournals.jbchem.a124046;
RA   Takagi T., Yasunaga H., Nakamura A.;
RT   "Structure of 29-kDa protein from ascidian (Halocynthia roretzi) body wall
RT   muscle.";
RL   J. Biochem. 113:321-326(1993).
RN   [2]
RP   SUBUNIT, AND SUBCELLULAR LOCATION.
RA   Shirakata M., Takagi T., Konishi K.;
RT   "Isolation and characterization of a 29,000 Dalton protein from ascidian
RT   (Halocynthia roretzi) body wall muscle.";
RL   Comp. Biochem. Physiol. 85B:71-76(1986).
CC   -!- FUNCTION: May be a component of myofibrils where it acts as a
CC       stabilizer.
CC   -!- SUBUNIT: Exists as an oligomer. {ECO:0000269|Ref.2}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000269|Ref.2}; Peripheral membrane
CC       protein {ECO:0000269|Ref.2}.
CC   -!- SIMILARITY: Belongs to the small heat shock protein (HSP20) family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00285}.
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DR   EMBL; D13917; BAA03012.1; -; mRNA.
DR   EMBL; D13918; BAA03013.1; -; Genomic_DNA.
DR   EMBL; S60426; AAA15484.2; -; Genomic_DNA.
DR   PIR; JX0258; JX0258.
DR   AlphaFoldDB; Q04757; -.
DR   SMR; Q04757; -.
DR   iPTMnet; Q04757; -.
DR   PRIDE; Q04757; -.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR   Gene3D; 2.60.40.790; -; 1.
DR   InterPro; IPR002068; A-crystallin/Hsp20_dom.
DR   InterPro; IPR001436; Alpha-crystallin/sHSP_animal.
DR   InterPro; IPR008978; HSP20-like_chaperone.
DR   PANTHER; PTHR45640; PTHR45640; 1.
DR   Pfam; PF00011; HSP20; 1.
DR   PRINTS; PR00299; ACRYSTALLIN.
DR   SUPFAM; SSF49764; SSF49764; 1.
DR   PROSITE; PS01031; SHSP; 1.
PE   1: Evidence at protein level;
KW   Acetylation; Direct protein sequencing; Membrane; Muscle protein; Repeat.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:8486605"
FT   CHAIN           2..252
FT                   /note="Body wall muscle protein HR-29"
FT                   /id="PRO_0000126061"
FT   REPEAT          37..55
FT                   /note="1"
FT   REPEAT          56..74
FT                   /note="2"
FT   REPEAT          75..93
FT                   /note="3"
FT   DOMAIN          138..249
FT                   /note="sHSP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00285"
FT   REGION          37..93
FT                   /note="3 X 19 AA approximate tandem repeats"
FT   MOD_RES         2
FT                   /note="N-acetylserine"
FT                   /evidence="ECO:0000269|PubMed:8486605"
SQ   SEQUENCE   252 AA;  28763 MW;  F177CB18D868C390 CRC64;
     MSLMPFDDFY YPYQLRRRSP RWMDSTGSPW DSFATSRDWM TTPYSSTGIG RRDLSQDWMT
     TPYTPAGVGR RDLSQDWMTT PYTSKGIGSR NLSSWGSKSE TIREMEKKFD ELVRKVDRRF
     TGMLSMLDDP EPFARQNISV EHEGKTTSKT TKLQDFNMKV DVQDFKPEEV KVKVQGGQVL
     VHAKRENRDE GDGMFAYSCS EFKRAFILPE GVSAERLTSS LSRDGILQID APVAVAIDNK
     KTAVPVTVEH TK