HR38_DROME
ID HR38_DROME Reviewed; 1073 AA.
AC P49869; O18383; Q9VIK4;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 3.
DT 03-AUG-2022, entry version 192.
DE RecName: Full=Probable nuclear hormone receptor HR38;
DE Short=dHR38;
DE AltName: Full=Nuclear receptor subfamily 4 group A member 4;
GN Name=Hr38; Synonyms=NR4A4; ORFNames=CG1864;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
RC TISSUE=Larva;
RX PubMed=7644522; DOI=10.1073/pnas.92.17.7966;
RA Sutherland J.D., Kozlova T., Tzertzinis G., Kafatos F.C.;
RT "Drosophila hormone receptor 38: a second partner for Drosophila USP
RT suggests an unexpected role for nuclear receptors of the nerve growth
RT factor-induced protein B type.";
RL Proc. Natl. Acad. Sci. U.S.A. 92:7966-7970(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM SHORT), AND TISSUE SPECIFICITY.
RX PubMed=9704500;
RX DOI=10.1002/(sici)1520-6327(1998)38:4<185::aid-arch4>3.0.co;2-q;
RA Komonyi O., Mink M., Csiha J., Maroy P.;
RT "Genomic organization of DHR38 gene in Drosophila: presence of Alu-like
RT repeat in a translated exon and expression during embryonic development.";
RL Arch. Insect Biochem. Physiol. 38:185-192(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG), AND CHARACTERIZATION.
RC TISSUE=Larva;
RX PubMed=9649534; DOI=10.1093/genetics/149.3.1465;
RA Kozlova T., Pokholkova G.V., Tzertzinis G., Sutherland J.D., Zhimulev I.F.,
RA Kafatos F.C.;
RT "Drosophila hormone receptor 38 functions in metamorphosis: a role in adult
RT cuticle formation.";
RL Genetics 149:1465-1475(1998).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORM LONG).
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 528-1073.
RC STRAIN=Canton-S;
RX PubMed=7479849; DOI=10.1073/pnas.92.23.10604;
RA Fisk G.J., Thummel C.S.;
RT "Isolation, regulation, and DNA-binding properties of three Drosophila
RT nuclear hormone receptor superfamily members.";
RL Proc. Natl. Acad. Sci. U.S.A. 92:10604-10608(1995).
CC -!- FUNCTION: Binds to NGFI-B response elements. Plays an important role in
CC late stages of epidermal metamorphosis.
CC -!- SUBUNIT: Forms a heterodimer with USP.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=Long;
CC IsoId=P49869-1; Sequence=Displayed;
CC Name=Short;
CC IsoId=P49869-2; Sequence=VSP_003714;
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed in preblastoderm embryos,
CC specifically in central nervous system and intestinal tract. Highly
CC expressed in third instar larval imaginal disks and brain complexes,
CC but not in ovaries. {ECO:0000269|PubMed:9704500}.
CC -!- DEVELOPMENTAL STAGE: Low levels in 0-8 hours embryos and adults. Higher
CC in late embryogenesis and during larval and pupal stages. Short isoform
CC is enriched in pupae and adults, long isoform in larvae.
CC -!- SIMILARITY: Belongs to the nuclear hormone receptor family. NR4
CC subfamily. {ECO:0000305}.
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DR EMBL; X89246; CAA61534.1; -; mRNA.
DR EMBL; Y15606; CAA75690.1; -; Genomic_DNA.
DR EMBL; AJ002073; CAA05172.1; -; mRNA.
DR EMBL; AE014134; AAF53914.1; -; Genomic_DNA.
DR EMBL; U36762; AAC46926.1; -; mRNA.
DR RefSeq; NP_477119.1; NM_057771.4.
DR PDB; 1PDU; X-ray; 2.30 A; A/B=842-1073.
DR PDBsum; 1PDU; -.
DR AlphaFoldDB; P49869; -.
DR SMR; P49869; -.
DR BioGRID; 61295; 10.
DR DIP; DIP-929N; -.
DR IntAct; P49869; 8.
DR STRING; 7227.FBpp0080927; -.
DR PaxDb; P49869; -.
DR PRIDE; P49869; -.
DR EnsemblMetazoa; FBtr0081397; FBpp0080927; FBgn0014859.
DR GeneID; 35332; -.
DR KEGG; dme:Dmel_CG1864; -.
DR CTD; 35332; -.
DR FlyBase; FBgn0014859; Hr38.
DR VEuPathDB; VectorBase:FBgn0014859; -.
DR eggNOG; KOG4217; Eukaryota.
DR GeneTree; ENSGT00950000183038; -.
DR InParanoid; P49869; -.
DR OrthoDB; 454476at2759; -.
DR Reactome; R-DME-198693; AKT phosphorylates targets in the nucleus.
DR Reactome; R-DME-383280; Nuclear Receptor transcription pathway.
DR SignaLink; P49869; -.
DR BioGRID-ORCS; 35332; 0 hits in 3 CRISPR screens.
DR ChiTaRS; Hr38; fly.
DR EvolutionaryTrace; P49869; -.
DR GenomeRNAi; 35332; -.
DR PRO; PR:P49869; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0014859; Expressed in head capsule and 8 other tissues.
DR ExpressionAtlas; P49869; baseline and differential.
DR Genevisible; P49869; DM.
DR GO; GO:0005737; C:cytoplasm; IMP:CAFA.
DR GO; GO:0005634; C:nucleus; IMP:CAFA.
DR GO; GO:0005667; C:transcription regulator complex; IBA:GO_Central.
DR GO; GO:0001046; F:core promoter sequence-specific DNA binding; IMP:CAFA.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0035259; F:nuclear glucocorticoid receptor binding; IBA:GO_Central.
DR GO; GO:0004879; F:nuclear receptor activity; NAS:FlyBase.
DR GO; GO:0046982; F:protein heterodimerization activity; IMP:CAFA.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:FlyBase.
DR GO; GO:0043565; F:sequence-specific DNA binding; IMP:CAFA.
DR GO; GO:0005102; F:signaling receptor binding; IPI:CAFA.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0071376; P:cellular response to corticotropin-releasing hormone stimulus; IBA:GO_Central.
DR GO; GO:0042335; P:cuticle development; IMP:FlyBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:FlyBase.
DR GO; GO:0048082; P:regulation of adult chitin-containing cuticle pigmentation; IGI:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR DisProt; DP00594; -.
DR Gene3D; 1.10.565.10; -; 1.
DR Gene3D; 3.30.50.10; -; 1.
DR InterPro; IPR035500; NHR-like_dom_sf.
DR InterPro; IPR000536; Nucl_hrmn_rcpt_lig-bd.
DR InterPro; IPR001723; Nuclear_hrmn_rcpt.
DR InterPro; IPR001628; Znf_hrmn_rcpt.
DR InterPro; IPR013088; Znf_NHR/GATA.
DR Pfam; PF00104; Hormone_recep; 1.
DR Pfam; PF00105; zf-C4; 1.
DR PRINTS; PR00398; STRDHORMONER.
DR PRINTS; PR00047; STROIDFINGER.
DR SMART; SM00430; HOLI; 1.
DR SMART; SM00399; ZnF_C4; 1.
DR SUPFAM; SSF48508; SSF48508; 1.
DR PROSITE; PS51843; NR_LBD; 1.
DR PROSITE; PS00031; NUCLEAR_REC_DBD_1; 1.
DR PROSITE; PS51030; NUCLEAR_REC_DBD_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Developmental protein; DNA-binding;
KW Metal-binding; Nucleus; Receptor; Reference proteome; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..1073
FT /note="Probable nuclear hormone receptor HR38"
FT /id="PRO_0000053725"
FT DOMAIN 840..1070
FT /note="NR LBD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01189"
FT DNA_BIND 741..816
FT /note="Nuclear receptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT ZN_FING 744..764
FT /note="NR C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT ZN_FING 780..804
FT /note="NR C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT REGION 55..87
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 150..185
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 263..326
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 437..458
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 492..514
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 529..579
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 618..640
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 819..841
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 55..81
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 263..279
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 290..326
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 537..579
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..522
FT /note="Missing (in isoform Short)"
FT /evidence="ECO:0000303|PubMed:7644522"
FT /id="VSP_003714"
FT CONFLICT 667
FT /note="V -> VSSPSV (in Ref. 4; AAF53914)"
FT /evidence="ECO:0000305"
FT CONFLICT 685
FT /note="S -> L (in Ref. 1 and 3)"
FT /evidence="ECO:0000305"
FT CONFLICT 689..692
FT /note="STAQ -> LHGER (in Ref. 2; CAA75690)"
FT /evidence="ECO:0000305"
FT CONFLICT 697
FT /note="A -> D (in Ref. 2; CAA75690)"
FT /evidence="ECO:0000305"
FT CONFLICT 702
FT /note="N -> S (in Ref. 2; CAA75690)"
FT /evidence="ECO:0000305"
FT CONFLICT 1041
FT /note="S -> R (in Ref. 2; CAA75690)"
FT /evidence="ECO:0000305"
FT CONFLICT 1064
FT /note="E -> D (in Ref. 2; CAA75690)"
FT /evidence="ECO:0000305"
FT HELIX 844..854
FT /evidence="ECO:0007829|PDB:1PDU"
FT HELIX 859..861
FT /evidence="ECO:0007829|PDB:1PDU"
FT TURN 865..867
FT /evidence="ECO:0007829|PDB:1PDU"
FT HELIX 874..896
FT /evidence="ECO:0007829|PDB:1PDU"
FT HELIX 902..904
FT /evidence="ECO:0007829|PDB:1PDU"
FT HELIX 907..929
FT /evidence="ECO:0007829|PDB:1PDU"
FT STRAND 935..938
FT /evidence="ECO:0007829|PDB:1PDU"
FT STRAND 942..946
FT /evidence="ECO:0007829|PDB:1PDU"
FT HELIX 947..954
FT /evidence="ECO:0007829|PDB:1PDU"
FT HELIX 957..971
FT /evidence="ECO:0007829|PDB:1PDU"
FT HELIX 975..986
FT /evidence="ECO:0007829|PDB:1PDU"
FT HELIX 996..1016
FT /evidence="ECO:0007829|PDB:1PDU"
FT HELIX 1018..1021
FT /evidence="ECO:0007829|PDB:1PDU"
FT HELIX 1026..1031
FT /evidence="ECO:0007829|PDB:1PDU"
FT HELIX 1033..1054
FT /evidence="ECO:0007829|PDB:1PDU"
FT HELIX 1061..1065
FT /evidence="ECO:0007829|PDB:1PDU"
FT TURN 1066..1068
FT /evidence="ECO:0007829|PDB:1PDU"
SQ SEQUENCE 1073 AA; 116992 MW; 126A30DAFA1C096A CRC64;
MMRDRLASLI VVKQEGGSNT SISHHQATAI KCEASLYTES SLFQEINNNS CYRQNLNAPT
HQQSHTSHLQ HAQQHQTHQQ HPLLPPPLPT LPLIYPCRNL FPDGCDINHL ACCSSSNSNS
NCNSDSNSTS SSPGNSHFFA NGNTCAAALT PAPPATEPRK IKPLGAGKLK VGKTDSNSDS
NSNCDSRAAA AASTSATSAT SATTLAATAA ATAAAAEAGG AASAAAAAKI SQVRLTNQAT
TSMLLLQPNS SFSSLSPFDN FSTQTASTTT TTSASAAGHH QHHNHLLHQQ HHNQQQQQQQ
QQQQQQQQQQ QQEHLQQQHQ QQLVSPQQHL LKSETLLSHE EDQLISNLTD SSVVSHSELF
SDLFFPSDSN NSLLSPTTSG YPDNPAEDLT SSIENLTKLT CLRDKRLSSI PEQQLSSEQE
QQLCLLSLRS SSDPAIALHA QQQQQQQQQQ QQQQQQHQQQ QQHLQLQLIS PIGGPLSCGS
SLPSFQETYS LKYNSSSGSS PQQASSSSTA APTPTDQVLT LKMDEDCFPP LSGGWSASPP
APSQLQQLHT LQSQAQMSHP NSSNNSSNNA GNSHNNSGGY NYHGHFNAIN ASANLSPSSS
ASSLYEYNGV SAADNFYGQQ QQQQQQSYQQ HNYNSHNGER YSLPTFPTIS ELAAATAAVE
AAAAATVGGP PPVRRASLPV QRTVSPAGST AQSPKLAKIT LNQRHSHAHA HALQLNSAPN
SAASSPASAD LQAGRLLQAP SQLCAVCGDT AACQHYGVRT CEGCKGFFKR TVQKGSKYVC
LADKNCPVDK RRRNRCQFCR FQKCLVVGMV KEVVRTDSLK GRRGRLPSKP KSPQESPPSP
PISLITALVR SHVDTTPDPS CLDYSHYEEQ SMSEADKVQQ FYQLLTSSVD VIKQFAEKIP
GYFDLLPEDQ ELLFQSASLE LFVLRLAYRA RIDDTKLIFC NGTVLHRTQC LRSFGEWLND
IMEFSRSLHN LEIDISAFAC LCALTLITER HGLREPKKVE QLQMKIIGSL RDHVTYNAEA
QKKQHYFSRL LGKLPELRSL SVQGLQRIFY LKLEDLVPAP ALIENMFVTT LPF
