HR4_ARATH
ID HR4_ARATH Reviewed; 200 AA.
AC Q9SCS6; Q0WNB7; Q570W3;
DT 07-JAN-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 110.
DE RecName: Full=RPW8-like protein 4;
DE Short=AtHR4 {ECO:0000303|PubMed:15155802};
GN Name=HR4; OrderedLocusNames=At3g50480 {ECO:0000312|Araport:AT3G50480};
GN ORFNames=T20E23.80 {ECO:0000312|EMBL:CAB62477.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=19140981; DOI=10.1111/j.1365-294x.2008.03960.x;
RA Jorgensen T.H., Emerson B.C.;
RT "Functional variation in a disease resistance gene in populations of
RT Arabidopsis thaliana.";
RL Mol. Ecol. 17:4912-4923(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP MISCELLANEOUS, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Ms-0;
RX PubMed=11141561; DOI=10.1126/science.291.5501.118;
RA Xiao S., Ellwood S., Calis O., Patrick E., Li T., Coleman M., Turner J.G.;
RT "Broad-spectrum mildew resistance in Arabidopsis thaliana mediated by
RT RPW8.";
RL Science 291:118-120(2001).
RN [7]
RP INDUCTION BY ERYSIPHE CICHORACEARUM, MISCELLANEOUS, AND GENE FAMILY.
RX PubMed=15155802; DOI=10.1093/molbev/msh165;
RA Xiao S., Emerson B., Ratanasut K., Patrick E., O'Neill C., Bancroft I.,
RA Turner J.G.;
RT "Origin and maintenance of a broad-spectrum disease resistance locus in
RT Arabidopsis.";
RL Mol. Biol. Evol. 21:1661-1672(2004).
RN [8]
RP INDUCTION BY PIERIS BRASSICAE OVIPOSITION.
RX PubMed=17142483; DOI=10.1104/pp.106.090837;
RA Little D., Gouhier-Darimont C., Bruessow F., Reymond P.;
RT "Oviposition by pierid butterflies triggers defense responses in
RT Arabidopsis.";
RL Plant Physiol. 143:784-800(2007).
RN [9]
RP INDUCTION BY MICROORGANISMS AND PHYTOHORMONES.
RC STRAIN=cv. Columbia;
RX PubMed=22942755; DOI=10.3390/ijms13079110;
RA Saenz-Mata J., Jimenez-Bremont J.F.;
RT "HR4 gene is induced in the Arabidopsis-trichoderma atroviride beneficial
RT interaction.";
RL Int. J. Mol. Sci. 13:9110-9128(2012).
CC -!- FUNCTION: Probable disease resistance (R) protein. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass membrane
CC protein {ECO:0000255}.
CC -!- INDUCTION: Expressed in leaves after powdery mildew infection (e.g.
CC Erysiphe cichoracearum UCSC1) (PubMed:15155802). Accumulates upon
CC oviposition by pierid butterflies (e.g. Pieris brassicae)
CC (PubMed:17142483). Induced in seedlings by the beneficial symbiotic
CC fungus Trichoderma atroviride and, by the plant growth-promoting
CC rhizobacterium (PGPR) Pseudomonas fluorescens after a transient
CC repression (PubMed:22942755). Expressed after infection by the
CC bacterial pathogenic Pseudomonas syringae pv. tomato DC3000
CC (PubMed:22942755). Triggered by various phytohormones such as ethylene
CC (ET), salicylic acid (SA) and methyl jasmonate (MeJA)
CC (PubMed:22942755). {ECO:0000269|PubMed:15155802,
CC ECO:0000269|PubMed:17142483, ECO:0000269|PubMed:22942755}.
CC -!- MISCELLANEOUS: Ecotypes susceptible to Erysiphe cichoracearum, such as
CC cv. Columbia, are lacking RPW8.1 and RPW8.2 but contain in place HR4.
CC {ECO:0000305|PubMed:11141561, ECO:0000305|PubMed:15155802}.
CC -!- SIMILARITY: Belongs to the plant RPW8 protein family. {ECO:0000305}.
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DR EMBL; FJ267632; ACJ05894.1; -; Genomic_DNA.
DR EMBL; AL133363; CAB62477.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE78671.1; -; Genomic_DNA.
DR EMBL; AF361849; AAK32861.1; -; mRNA.
DR EMBL; BT000848; AAN38685.1; -; mRNA.
DR EMBL; AK220594; BAD94912.1; -; mRNA.
DR EMBL; AK229528; BAF01383.1; -; mRNA.
DR PIR; T46079; T46079.
DR RefSeq; NP_566933.1; NM_114908.5.
DR AlphaFoldDB; Q9SCS6; -.
DR SMR; Q9SCS6; -.
DR STRING; 3702.AT3G50480.1; -.
DR PaxDb; Q9SCS6; -.
DR PRIDE; Q9SCS6; -.
DR ProteomicsDB; 230234; -.
DR EnsemblPlants; AT3G50480.1; AT3G50480.1; AT3G50480.
DR GeneID; 824212; -.
DR Gramene; AT3G50480.1; AT3G50480.1; AT3G50480.
DR KEGG; ath:AT3G50480; -.
DR Araport; AT3G50480; -.
DR TAIR; locus:2098740; AT3G50480.
DR HOGENOM; CLU_1367877_0_0_1; -.
DR PhylomeDB; Q9SCS6; -.
DR PRO; PR:Q9SCS6; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9SCS6; baseline and differential.
DR Genevisible; Q9SCS6; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0050832; P:defense response to fungus; IEA:InterPro.
DR GO; GO:0009626; P:plant-type hypersensitive response; IEA:UniProtKB-KW.
DR GO; GO:0009617; P:response to bacterium; IEP:UniProtKB.
DR GO; GO:0009723; P:response to ethylene; IEP:UniProtKB.
DR GO; GO:0043207; P:response to external biotic stimulus; IEP:UniProtKB.
DR GO; GO:0009620; P:response to fungus; IEP:UniProtKB.
DR GO; GO:0009753; P:response to jasmonic acid; IEP:UniProtKB.
DR GO; GO:0009751; P:response to salicylic acid; IEP:UniProtKB.
DR GO; GO:0009609; P:response to symbiotic bacterium; IEP:UniProtKB.
DR GO; GO:0009610; P:response to symbiotic fungus; IEP:UniProtKB.
DR InterPro; IPR008808; Powdery_mildew-R_dom.
DR InterPro; IPR039203; RPW8-like.
DR PANTHER; PTHR36766; PTHR36766; 4.
DR Pfam; PF05659; RPW8; 1.
DR PROSITE; PS51153; RPW8; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; Hypersensitive response; Membrane; Plant defense;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..200
FT /note="RPW8-like protein 4"
FT /id="PRO_0000431674"
FT TRANSMEM 7..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 1..157
FT /note="RPW8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00495"
FT COILED 70..127
FT /evidence="ECO:0000255"
FT CONFLICT 118
FT /note="R -> W (in Ref. 5; BAF01383)"
FT /evidence="ECO:0000305"
FT CONFLICT 198..200
FT /note="HNK -> Q (in Ref. 5; BAD94912)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 200 AA; 23389 MW; 6D18726A79A3C679 CRC64;
MPIAELAVIK TVGGPLIAAA LGVGAQVIYD GFRKGKDTSI INRLGRTMES ISPVRDRIGK
LSNVEGKPFR EVHESLTRLL EDAKSIIEKY WKLRWSRHVC RKYRYIKKLE SIELELVRVA
REIQVHQWTD IKEMKAIQVH QWTDIKEMKA IQVDQWTDIK EMKAIQVDQW IDIKEMKAIQ
VDQWTDIKEM KAQISEKHNK
