HR4_DROME
ID HR4_DROME Reviewed; 1518 AA.
AC Q9W539; O96836; Q56AW0;
DT 10-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2005, sequence version 4.
DT 03-AUG-2022, entry version 169.
DE RecName: Full=Hormone receptor 4;
DE Short=dHR4;
DE AltName: Full=Nuclear receptor subfamily 6 group A member 2;
GN Name=Hr4; Synonyms=NR6A2; ORFNames=CG43934;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND DISRUPTION PHENOTYPE.
RC TISSUE=Embryo;
RX PubMed=15935763; DOI=10.1016/j.cell.2005.03.030;
RA King-Jones K., Charles J.P., Lam G., Thummel C.S.;
RT "The ecdysone-induced DHR4 orphan nuclear receptor coordinates growth and
RT maturation in Drosophila.";
RL Cell 121:773-784(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:10731132};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3] {ECO:0000305}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4] {ECO:0000312|EMBL:CAA22836.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Oregon-R {ECO:0000269|PubMed:10731137};
RX PubMed=10731137; DOI=10.1126/science.287.5461.2220;
RA Benos P.V., Gatt M.K., Ashburner M., Murphy L., Harris D., Barrell B.G.,
RA Ferraz C., Vidal S., Brun C., Demailles J., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Borkova D., Minana B., Kafatos F.C.,
RA Louis C., Siden-Kiamos I., Bolshakov S., Papagiannakis G., Spanos L.,
RA Cox S., Madueno E., de Pablos B., Modolell J., Peter A., Schoettler P.,
RA Werner M., Mourkioti F., Beinert N., Dowe G., Schaefer U., Jaeckle H.,
RA Bucheton A., Callister D.M., Campbell L.A., Darlamitsou A., Henderson N.S.,
RA McMillan P.J., Salles C., Tait E.A., Valenti P., Saunders R.D.C.,
RA Glover D.M.;
RT "From sequence to chromosome: the tip of the X chromosome of D.
RT melanogaster.";
RL Science 287:2220-2222(2000).
CC -!- FUNCTION: Coordinates growth and maturation by mediating endocrine
CC responses to the attainment of critical weight during larval
CC development. Plays a central role in the genetic cascades triggered by
CC the steroid hormone ecdysone at the onset of metamorphosis, acting as
CC both a repressor of the early ecdysone-induced regulatory genes and an
CC inducer of the ftz-f1 midprepupal competence factor.
CC {ECO:0000269|PubMed:15935763}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00407,
CC ECO:0000269|PubMed:15935763}.
CC -!- TISSUE SPECIFICITY: During L2 and L3 stages, strong constitutive
CC expression is seen in the ring gland. Lower expression is detected in
CC specific neurons of the central nervous system (CNS) (at protein
CC level). {ECO:0000269|PubMed:15935763}.
CC -!- DISRUPTION PHENOTYPE: Flies exhibit larvae that precociously leave the
CC food to form premature prepupae, resulting in abbreviated larval
CC development that translates directly into smaller and lighter animals.
CC {ECO:0000269|PubMed:15935763}.
CC -!- SIMILARITY: Belongs to the nuclear hormone receptor family. NR1
CC subfamily. {ECO:0000255}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA22836.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY971884; AAX73355.1; -; mRNA.
DR EMBL; AE014298; ABC67166.1; -; Genomic_DNA.
DR EMBL; AL035245; CAA22836.1; ALT_SEQ; Genomic_DNA.
DR RefSeq; NP_001033823.1; NM_001038734.2.
DR AlphaFoldDB; Q9W539; -.
DR BioGRID; 57707; 10.
DR STRING; 7227.FBpp0305529; -.
DR PaxDb; Q9W539; -.
DR EnsemblMetazoa; FBtr0333341; FBpp0305533; FBgn0264562.
DR GeneID; 31162; -.
DR KEGG; dme:Dmel_CG43934; -.
DR UCSC; CG16902-RC; d. melanogaster.
DR CTD; 31162; -.
DR FlyBase; FBgn0264562; Hr4.
DR VEuPathDB; VectorBase:FBgn0264562; -.
DR eggNOG; KOG3575; Eukaryota.
DR GeneTree; ENSGT00940000157936; -.
DR HOGENOM; CLU_001479_0_0_1; -.
DR InParanoid; Q9W539; -.
DR PhylomeDB; Q9W539; -.
DR Reactome; R-DME-383280; Nuclear Receptor transcription pathway.
DR BioGRID-ORCS; 31162; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 31162; -.
DR PRO; PR:Q9W539; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0264562; Expressed in saliva-secreting gland and 15 other tissues.
DR ExpressionAtlas; Q9W539; baseline and differential.
DR Genevisible; Q9W539; DM.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0004879; F:nuclear receptor activity; IMP:UniProtKB.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0071456; P:cellular response to hypoxia; IMP:FlyBase.
DR GO; GO:0120143; P:negative regulation of ecdysone receptor-mediated signaling pathway; IMP:UniProtKB.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IMP:UniProtKB.
DR GO; GO:0040018; P:positive regulation of multicellular organism growth; IMP:UniProtKB.
DR GO; GO:0048638; P:regulation of developmental growth; IMP:FlyBase.
DR GO; GO:0106023; P:regulation of pupariation; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 1.10.565.10; -; 1.
DR Gene3D; 3.30.50.10; -; 1.
DR InterPro; IPR035500; NHR-like_dom_sf.
DR InterPro; IPR000536; Nucl_hrmn_rcpt_lig-bd.
DR InterPro; IPR001723; Nuclear_hrmn_rcpt.
DR InterPro; IPR001628; Znf_hrmn_rcpt.
DR InterPro; IPR013088; Znf_NHR/GATA.
DR Pfam; PF00104; Hormone_recep; 1.
DR Pfam; PF00105; zf-C4; 1.
DR PRINTS; PR00398; STRDHORMONER.
DR PRINTS; PR00047; STROIDFINGER.
DR SMART; SM00430; HOLI; 1.
DR SMART; SM00399; ZnF_C4; 1.
DR SUPFAM; SSF48508; SSF48508; 1.
DR PROSITE; PS51843; NR_LBD; 1.
DR PROSITE; PS00031; NUCLEAR_REC_DBD_1; 1.
DR PROSITE; PS51030; NUCLEAR_REC_DBD_2; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Metal-binding; Nucleus; Receptor; Reference proteome;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..1518
FT /note="Hormone receptor 4"
FT /id="PRO_0000053522"
FT DOMAIN 1250..1518
FT /note="NR LBD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01189"
FT DNA_BIND 918..993
FT /note="Nuclear receptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT ZN_FING 921..941
FT /note="NR C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT ZN_FING 957..976
FT /note="NR C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT REGION 30..50
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 145..327
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 380..587
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 672..820
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 887..913
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1015..1101
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1142..1210
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1341..1371
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 34..49
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 145..218
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 249..327
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 399..431
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 432..527
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 542..587
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 680..713
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 740..805
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1022..1058
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1059..1074
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1075..1101
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1148..1195
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 140
FT /note="T -> R (in Ref. 1; CAA22836)"
FT /evidence="ECO:0000305"
FT CONFLICT 144
FT /note="A -> R (in Ref. 1; CAA22836)"
FT /evidence="ECO:0000305"
FT CONFLICT 213
FT /note="I -> V (in Ref. 1; CAA22836)"
FT /evidence="ECO:0000305"
FT CONFLICT 1452
FT /note="K -> KA (in Ref. 1; CAA22836)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1518 AA; 162655 MW; DDBDFEBA416D17AA CRC64;
MTLSRGPYSE LDKMSLFQDL KLKRRKIDSR CSSDGESIAD TSTSSPDLLA PMSPKLCDSG
SAGASLGASL PLPLALPLPM ALPLPMSLPL PLTAASSAVT VSLAAVVAAV AETGGAGAGG
AGTAVTASGA GPCVSTSSTT AAAATSSTSS LSSSSSSSSS TSSSTSSASP TAGASSTATC
PASSSSSSGN GSGGKSGSIK QEHTEIHSSS SAISAAAAST VMSPPPAEAT RSSPATPEGG
GPAGDGSGAT GGGNTSGGST AGVAINEHQN NGNGSGGSSR ASPDSLEEKP STTTTTGRPT
LTPTNGVLSS ASAGTGISTG SSAKLSEAGM SVIRSVKEER LLNVSSKMLV FHQQREQETK
AVAAAAAAAA AGHVTVLVTP SRIKSEPPPP ASPSSTSSTQ RERERERDRE RDRERERERD
RDREREREQS ISSSQQHLSR VSASPPTQLS HGSLGPNIVQ THHLHQQLTQ PLTLRKSSPP
TEHLLSQSMQ HLTQQQAIHL HHLLGQQQQQ QQASHPQQQQ QQQHSPHSLV RVKKEPNVGQ
RHLSPHHQQQ SPLLQHHQQQ QQQQQQQQQH LHQQQQQQQH HQQQPQALAL MHPASLALRN
SNRDAAILFR VKSEVHQQVA AGLPHLMQSA GGAAAAAAAA VAAQRMVCFS NARINGVKPE
VIGGPLGNLR PVGVGGGNGS GSVQCPSPHP SSSSSSSQLS PQTPSQTPPR GTPTVIMGES
CGVRTMVWGY EPPPPSAGQS HGQHPQQQQQ SPHHQPQQQQ QQQQQQSQQQ QQQQQQQSLG
QQQHCLSSPS AGSLTPSSSS GGGSVSGGGV GGPLTPSSVA PQNNEEAAQL LLSLGQTRIQ
DMRSRPHPFR TPHALNMERL WAGDYSQLPP GQLQALNLSA QQQQWGSSNS TGLGGVGGGM
GGRNLEAPHE PTDEDEQPLV CMICEDKATG LHYGIITCEG CKGFFKRTVQ NRRVYTCVAD
GTCEITKAQR NRCQYCRFKK CIEQGMVLQA VREDRMPGGR NSGAVYNLYK VKYKKHKKTN
QKQQQQAAQQ QQQQAAAQQQ HQQQQQHQQH QQHQQQQLHS PLHHHHHQGH QSHHAQQQHH
PQLSPHHLLS PQQQQLAAAV AAAAQHQQQQ QQQQQQQQQA KLMGGVVDMK PMFLGPALKP
ELLQAPPMHS PAQQQQQQQQ QQQQQQASPH LSLSSPHQQQ QQQQGQHQNH HQQQGGGGGG
AGGGAQLPPH LVNGTILKTA LTNPSEIVHL RHRLDSAVSS SKDRQISYEH ALGMIQTLID
CDAMEDIATL PHFSEFLEDK SEISEKLCNI GDSIVHKLVS WTKKLPFYLE IPVEIHTKLL
TDKWHEILIL TTAAYQALHG KRRGEGGGSR HGSPASTPLS TPTGTPLSTP IPSPAQPLHK
DDPEFVSEVN SHLSTLQTCL TTLMGQPIAM EQLKLDVGHM VDKMTQITIM FRRIKLKMEE
YVCLKVYILL NKEVELESIQ ERYVQVLRSY LQNSSPQNPQ ARLSELLSHI PEIQAAASLL
LESKMFYVPF VLNSASIR
