AP1S1_ARATH
ID AP1S1_ARATH Reviewed; 161 AA.
AC Q8LEZ8; O24611;
DT 05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 25-MAY-2022, entry version 115.
DE RecName: Full=AP-1 complex subunit sigma-1;
DE AltName: Full=Adaptor AP-1 19 kDa protein;
DE AltName: Full=Adaptor protein complex AP-1 sigma-1 subunit;
DE AltName: Full=Adaptor-related protein complex 1 sigma-1 subunit;
DE AltName: Full=Clathrin assembly protein complex 1 sigma-1 small chain;
DE AltName: Full=Clathrin assembly small subunit protein AP19-1;
DE Short=AtAP19-1;
DE AltName: Full=Sigma 1 subunit of AP-1 clathrin;
DE AltName: Full=Sigma-adaptin 1;
DE AltName: Full=Sigma1-adaptin;
GN Name=AAP19-1; OrderedLocusNames=At2g17380; ORFNames=F5J6.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=cv. Landsberg erecta; TISSUE=Leaf;
RX PubMed=9426606; DOI=10.1023/a:1005923829224;
RA Maldonado-Mendoza I.E., Nessler C.L.;
RT "Molecular characterization of the AP19 gene family in Arabidopsis
RT thaliana: components of the Golgi AP-1 clathrin assembly protein complex.";
RL Plant Mol. Biol. 35:865-872(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY, AND REVIEW.
RX PubMed=11598180; DOI=10.1091/mbc.12.10.2907;
RA Boehm M., Bonifacino J.S.;
RT "Adaptins: the final recount.";
RL Mol. Biol. Cell 12:2907-2920(2001).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND COMPONENT OF THE AP-1 COMPLEX.
RX PubMed=23543752; DOI=10.1093/pcp/pct048;
RA Teh O.K., Shimono Y., Shirakawa M., Fukao Y., Tamura K., Shimada T.,
RA Hara-Nishimura I.;
RT "The AP-1 mu adaptin is required for KNOLLE localization at the cell plate
RT to mediate cytokinesis in Arabidopsis.";
RL Plant Cell Physiol. 54:838-847(2013).
CC -!- FUNCTION: Subunit of clathrin-associated adaptor protein complex 1 that
CC plays a role in protein sorting at the trans-Golgi network and early
CC endosomes (TGN/EE). The AP complexes mediate the recruitment of
CC clathrin to membranes and the recognition of sorting signals within the
CC cytosolic tails of transmembrane cargo molecules (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Adaptor protein complex 1 (AP-1) is a heterotetramer composed
CC of two large adaptins (gamma-type subunit and beta-type subunit), a
CC medium adaptin (mu-type subunit) and a small adaptin (sigma-type
CC subunit).
CC -!- SUBCELLULAR LOCATION: Golgi apparatus {ECO:0000250}. Cytoplasmic
CC vesicle, clathrin-coated vesicle membrane {ECO:0000250}; Peripheral
CC membrane protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in seedlings, roots, stems, leaves,
CC flowers and siliques (developing fruits and seeds).
CC {ECO:0000269|PubMed:9426606}.
CC -!- SIMILARITY: Belongs to the adaptor complexes small subunit family.
CC {ECO:0000305}.
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DR EMBL; U92084; AAB96887.1; -; mRNA.
DR EMBL; U92085; AAB96888.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC06619.1; -; Genomic_DNA.
DR EMBL; AY085130; AAM61683.1; -; mRNA.
DR EMBL; BT025619; ABF59037.1; -; mRNA.
DR PIR; E84551; E84551.
DR RefSeq; NP_565415.1; NM_127293.3.
DR AlphaFoldDB; Q8LEZ8; -.
DR SMR; Q8LEZ8; -.
DR BioGRID; 1602; 9.
DR STRING; 3702.AT2G17380.1; -.
DR PaxDb; Q8LEZ8; -.
DR PRIDE; Q8LEZ8; -.
DR ProteomicsDB; 245028; -.
DR EnsemblPlants; AT2G17380.1; AT2G17380.1; AT2G17380.
DR GeneID; 816245; -.
DR Gramene; AT2G17380.1; AT2G17380.1; AT2G17380.
DR KEGG; ath:AT2G17380; -.
DR Araport; AT2G17380; -.
DR TAIR; locus:2827550; AT2G17380.
DR eggNOG; KOG0934; Eukaryota.
DR HOGENOM; CLU_061221_1_3_1; -.
DR InParanoid; Q8LEZ8; -.
DR OMA; LAKWYAT; -.
DR OrthoDB; 1307450at2759; -.
DR PhylomeDB; Q8LEZ8; -.
DR PRO; PR:Q8LEZ8; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8LEZ8; baseline and differential.
DR Genevisible; Q8LEZ8; AT.
DR GO; GO:0030121; C:AP-1 adaptor complex; IEA:InterPro.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IBA:GO_Central.
DR GO; GO:0035615; F:clathrin adaptor activity; IEA:InterPro.
DR GO; GO:0006886; P:intracellular protein transport; IEA:InterPro.
DR GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR CDD; cd14831; AP1_sigma; 1.
DR InterPro; IPR044733; AP1_sigma.
DR InterPro; IPR016635; AP_complex_ssu.
DR InterPro; IPR022775; AP_mu_sigma_su.
DR InterPro; IPR000804; Clathrin_sm-chain_CS.
DR InterPro; IPR011012; Longin-like_dom_sf.
DR PANTHER; PTHR11753; PTHR11753; 1.
DR Pfam; PF01217; Clat_adaptor_s; 1.
DR PIRSF; PIRSF015588; AP_complex_sigma; 1.
DR SUPFAM; SSF64356; SSF64356; 1.
DR PROSITE; PS00989; CLAT_ADAPTOR_S; 1.
PE 1: Evidence at protein level;
KW Cytoplasmic vesicle; Golgi apparatus; Membrane; Protein transport;
KW Reference proteome; Transport.
FT CHAIN 1..161
FT /note="AP-1 complex subunit sigma-1"
FT /id="PRO_0000397856"
FT CONFLICT 77
FT /note="E -> A (in Ref. 1; AAB96887/AAB96888)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 161 AA; 18759 MW; CAFA1C059AED2847 CRC64;
MIHFVLLVSR QGKVRLTKWY SPYTQKERSK VIRELSGVIL NRGPKLCNFI EWRGYKVVYK
RYASLYFCMC IDEADNELEV LEIIHHYVEI LDRYFGSVCE LDLIFNFHKA YYILDELLIA
GELQESSKKT VARIISAQDQ LVEVAKEEAS SISNIIAQAT K
