AP1S3_MOUSE
ID AP1S3_MOUSE Reviewed; 154 AA.
AC Q7TN05;
DT 13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=AP-1 complex subunit sigma-3;
DE AltName: Full=Adaptor protein complex AP-1 subunit sigma-1C;
DE AltName: Full=Adaptor-related protein complex 1 subunit sigma-1C;
DE AltName: Full=Clathrin assembly protein complex 1 sigma-1C small chain;
DE AltName: Full=Golgi adaptor HA1/AP1 adaptin sigma-1C subunit;
DE AltName: Full=Sigma 1C subunit of AP-1 clathrin;
DE AltName: Full=Sigma-adaptin 1C;
DE AltName: Full=Sigma1C-adaptin;
GN Name=Ap1s3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=129, and C57BL/6J; TISSUE=Mammary tumor, and Thymus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney, Lung, and Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Subunit of clathrin-associated adaptor protein complex 1 that
CC plays a role in protein sorting in the late-Golgi/trans-Golgi network
CC (TGN) and/or endosomes. The AP complexes mediate both the recruitment
CC of clathrin to membranes and the recognition of sorting signals within
CC the cytosolic tails of transmembrane cargo molecules (By similarity).
CC Involved in TLR3 trafficking (By similarity).
CC {ECO:0000250|UniProtKB:Q96PC3}.
CC -!- SUBUNIT: Adaptor protein complex 1 (AP-1) is a heterotetramer composed
CC of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit
CC AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small
CC adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus. Cytoplasmic vesicle membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic
CC side {ECO:0000250}. Membrane, clathrin-coated pit {ECO:0000250}.
CC Note=Component of the coat surrounding the cytoplasmic face of coated
CC vesicles located at the Golgi complex. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the adaptor complexes small subunit family.
CC {ECO:0000305}.
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DR EMBL; BC054111; AAH54111.1; -; mRNA.
DR EMBL; BC098237; AAH98237.1; -; mRNA.
DR CCDS; CCDS35628.1; -.
DR RefSeq; NP_898848.1; NM_183027.2.
DR AlphaFoldDB; Q7TN05; -.
DR SMR; Q7TN05; -.
DR ComplexPortal; CPX-5143; Ubiquitous AP-1 Adaptor complex, sigma1c variant.
DR STRING; 10090.ENSMUSP00000125268; -.
DR PhosphoSitePlus; Q7TN05; -.
DR EPD; Q7TN05; -.
DR MaxQB; Q7TN05; -.
DR PaxDb; Q7TN05; -.
DR PRIDE; Q7TN05; -.
DR ProteomicsDB; 281893; -.
DR Antibodypedia; 54195; 104 antibodies from 14 providers.
DR DNASU; 252903; -.
DR Ensembl; ENSMUST00000162342; ENSMUSP00000125268; ENSMUSG00000054702.
DR GeneID; 252903; -.
DR KEGG; mmu:252903; -.
DR UCSC; uc011wnw.1; mouse.
DR CTD; 130340; -.
DR MGI; MGI:1891304; Ap1s3.
DR VEuPathDB; HostDB:ENSMUSG00000054702; -.
DR eggNOG; KOG0934; Eukaryota.
DR GeneTree; ENSGT00970000193372; -.
DR HOGENOM; CLU_061221_1_3_1; -.
DR InParanoid; Q7TN05; -.
DR OMA; LAKWYAT; -.
DR OrthoDB; 1307450at2759; -.
DR PhylomeDB; Q7TN05; -.
DR TreeFam; TF312921; -.
DR Reactome; R-MMU-2132295; MHC class II antigen presentation.
DR Reactome; R-MMU-432720; Lysosome Vesicle Biogenesis.
DR Reactome; R-MMU-432722; Golgi Associated Vesicle Biogenesis.
DR BioGRID-ORCS; 252903; 0 hits in 72 CRISPR screens.
DR PRO; PR:Q7TN05; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q7TN05; protein.
DR Bgee; ENSMUSG00000054702; Expressed in epiblast (generic) and 62 other tissues.
DR ExpressionAtlas; Q7TN05; baseline and differential.
DR Genevisible; Q7TN05; MM.
DR GO; GO:0030121; C:AP-1 adaptor complex; IC:ComplexPortal.
DR GO; GO:0005905; C:clathrin-coated pit; IEA:UniProtKB-SubCell.
DR GO; GO:0005769; C:early endosome; IC:ComplexPortal.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0005765; C:lysosomal membrane; IC:ComplexPortal.
DR GO; GO:0032588; C:trans-Golgi network membrane; IC:ComplexPortal.
DR GO; GO:0035615; F:clathrin adaptor activity; IEA:InterPro.
DR GO; GO:1903232; P:melanosome assembly; IC:ComplexPortal.
DR GO; GO:0060155; P:platelet dense granule organization; IC:ComplexPortal.
DR GO; GO:0006605; P:protein targeting; ISS:UniProtKB.
DR GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR CDD; cd14831; AP1_sigma; 1.
DR InterPro; IPR044733; AP1_sigma.
DR InterPro; IPR016635; AP_complex_ssu.
DR InterPro; IPR022775; AP_mu_sigma_su.
DR InterPro; IPR000804; Clathrin_sm-chain_CS.
DR InterPro; IPR011012; Longin-like_dom_sf.
DR PANTHER; PTHR11753; PTHR11753; 1.
DR Pfam; PF01217; Clat_adaptor_s; 1.
DR PIRSF; PIRSF015588; AP_complex_sigma; 1.
DR SUPFAM; SSF64356; SSF64356; 1.
DR PROSITE; PS00989; CLAT_ADAPTOR_S; 1.
PE 1: Evidence at protein level;
KW Coated pit; Cytoplasmic vesicle; Golgi apparatus; Membrane;
KW Protein transport; Reference proteome; Transport.
FT CHAIN 1..154
FT /note="AP-1 complex subunit sigma-3"
FT /id="PRO_0000193802"
SQ SEQUENCE 154 AA; 18440 MW; 231E454DDECBB302 CRC64;
MIHFILLFSR QGKLRLQKWY TTLPDKERKK ITRDIIQTVL SRGHRTSSFI DWKELKLVYK
RYASLYFCCA IENQDNELLT LEIVHRYVEL LDKYFGNVCE LDIIFNFEKA YFILDEFIIG
GEIQETSKKT AVKAIEDSDM LQETMEEYMN KPTF
