ID   3SX1_MICMP              Reviewed;          81 AA.
AC   B3EWF8; A0A2P1BSY2;
DT   05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT   12-AUG-2020, sequence version 2.
DT   25-MAY-2022, entry version 32.
DE   RecName: Full=Mipartoxin-1 {ECO:0000303|PubMed:31557485};
DE   AltName: Full=Mipartoxin-I {ECO:0000303|PubMed:22677806};
DE   AltName: Full=Three-finger toxin;
DE            Short=3FTx;
DE   Flags: Precursor;
OS   Micrurus mipartitus (Red-tailed coral snake).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Elapidae; Elapinae; Micrurus.
OX   NCBI_TaxID=430902;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC   TISSUE=Venom gland {ECO:0000303|PubMed:31557485};
RX   PubMed=31557485; DOI=10.1016/j.toxicon.2019.09.017;
RA   Rey-Suarez P., Saldarriaga-Cordoba M., Torres U., Marin-Villa M.,
RA   Lomonte B., Nunez V.;
RT   "Novel three-finger toxins from Micrurus dumerilii and Micrurus mipartitus
RT   coral snake venoms: Phylogenetic relationships and characterization of
RT   Clarkitoxin-I-Mdum.";
RL   Toxicon 170:85-93(2019).
RN   [2]
RP   PROTEIN SEQUENCE OF 22-81, FUNCTION, BIOASSAY, SUBCELLULAR LOCATION, MASS
RP   SPECTROMETRY, DISULFIDE BONDS, AND TOXIC DOSE.
RC   TISSUE=Venom;
RX   PubMed=22677806; DOI=10.1016/j.toxicon.2012.05.023;
RA   Rey-Suarez P., Floriano R.S., Rostelato-Ferreira S.,
RA   Saldarriaga-Cordoba M., Nunez V., Rodrigues-Simioni L., Lomonte B.;
RT   "Mipartoxin-I, a novel three-finger toxin, is the major neurotoxic
RT   component in the venom of the redtail coral snake Micrurus mipartitus
RT   (Elapidae).";
RL   Toxicon 60:851-863(2012).
CC   -!- FUNCTION: Snake venom neurotoxin that blocks neuromuscular transmission
CC       on both avian and mouse nerve-muscle preparations, presenting a
CC       postsynaptic action through the nicotinic acetylcholine receptor
CC       (nAChR). Reversibly inhibits twitches in mouse phrenic nerve diaphragm
CC       and irreversibly in chick biventer cervicis muscle. Has no cytotoxic
CC       activity towards C2C12 cells up to 180 ug/ml.
CC       {ECO:0000269|PubMed:22677806}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:22677806}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000269|PubMed:31557485}.
CC   -!- PTM: Contains 4 disulfide bonds. {ECO:0000269|PubMed:22677806}.
CC   -!- MASS SPECTROMETRY: Mass=7029.7; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:22677806};
CC   -!- MASS SPECTROMETRY: Mass=7030.0; Mass_error=0.5; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:22677806};
CC   -!- TOXIC DOSE: LD(50) is 0.06 mg/kg by intraperitoneal injection into
CC       mice. {ECO:0000269|PubMed:22677806}.
CC   -!- SIMILARITY: Belongs to the snake three-finger toxin family. Short-chain
CC       subfamily. {ECO:0000305}.
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DR   EMBL; KY635900; AVI57319.1; -; mRNA.
DR   AlphaFoldDB; B3EWF8; -.
DR   SMR; B3EWF8; -.
DR   GO; GO:0005615; C:extracellular space; IDA:UniProtKB.
DR   GO; GO:0030550; F:acetylcholine receptor inhibitor activity; IDA:UniProtKB.
DR   GO; GO:0099106; F:ion channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IDA:UniProtKB.
DR   GO; GO:0035738; P:envenomation resulting in modulation of process in another organism; IDA:UniProtKB.
DR   GO; GO:0035821; P:modulation of process of another organism; IDA:UniProtKB.
DR   GO; GO:2000272; P:negative regulation of signaling receptor activity; IDA:UniProtKB.
DR   Gene3D; 2.10.60.10; -; 1.
DR   InterPro; IPR045860; Snake_toxin-like_sf.
DR   SUPFAM; SSF57302; SSF57302; 1.
PE   1: Evidence at protein level;
KW   Acetylcholine receptor inhibiting toxin; Direct protein sequencing;
KW   Disulfide bond; Ion channel impairing toxin; Neurotoxin;
KW   Postsynaptic neurotoxin; Secreted; Signal; Toxin.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..81
FT                   /note="Mipartoxin-1"
FT                   /evidence="ECO:0000269|PubMed:22677806"
FT                   /id="PRO_0000418720"
FT   DISULFID        24..42
FT                   /evidence="ECO:0000250|UniProtKB:P07526"
FT   DISULFID        35..61
FT                   /evidence="ECO:0000250|UniProtKB:P07526"
FT   DISULFID        65..73
FT                   /evidence="ECO:0000250|UniProtKB:P07526"
FT   DISULFID        74..79
FT                   /evidence="ECO:0000250|UniProtKB:P07526"
SQ   SEQUENCE   81 AA;  9266 MW;  AA26F21D4168F78F CRC64;
     MKTLLLTLVV VTIVCLDLGN SLKCYVSREG KTQTCPEGEK LCEKYAVSYF HDGRWRYRYE
     CTSACHRGPY NVCCSTDLCN K