AP1_ARATH
ID AP1_ARATH Reviewed; 256 AA.
AC P35631; Q680D4; Q8LA98; Q9LQA8;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 20-JUN-2002, sequence version 2.
DT 03-AUG-2022, entry version 175.
DE RecName: Full=Floral homeotic protein APETALA 1;
DE AltName: Full=Agamous-like MADS-box protein AGL7;
GN Name=AP1; Synonyms=AGL7; OrderedLocusNames=At1g69120; ORFNames=F4N2.9;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Landsberg erecta;
RX PubMed=1359429; DOI=10.1038/360273a0;
RA Mandel M.A., Gustafson-Brown C., Savidge B., Yanofsky M.F.;
RT "Molecular characterization of the Arabidopsis floral homeotic gene
RT APETALA1.";
RL Nature 360:273-277(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-251, AND VARIANTS.
RC STRAIN=cv. Bla-1, cv. Bretagny, cv. Bs-1, cv. Bu-0, cv. Bu-2, cv. Chi-1,
RC cv. Co-1, cv. Columbia, cv. Cvi-0, cv. Gr-3, cv. Ita-0, cv. Jl-1,
RC cv. Kas-1, cv. Kent, cv. Landsberg erecta, cv. Li-3, cv. Li-8, cv. Lisse,
RC and cv. Wassilewskija;
RX PubMed=11973317; DOI=10.1093/genetics/160.4.1641;
RA Olsen K.M., Womack A., Garrett A.R., Suddith J.I., Purugganan M.D.;
RT "Contrasting evolutionary forces in the Arabidopsis thaliana floral
RT developmental pathway.";
RL Genetics 160:1641-1650(2002).
RN [8]
RP FUNCTION.
RA Bowman J.L., Alvarez J., Weigel D., Meyerowitz E.M., Smyth D.R.;
RT "Control of flower development in Arabidopsis thaliana by APETALA1 and
RT interacting genes.";
RL Development 119:721-743(1993).
RN [9]
RP FUNCTION.
RX PubMed=17794879; DOI=10.1126/science.261.5129.1723;
RA Weigel D., Meyerowitz E.M.;
RT "Activation of floral homeotic genes in Arabidopsis.";
RL Science 261:1723-1726(1993).
RN [10]
RP CHARACTERIZATION.
RX PubMed=8643482; DOI=10.1073/pnas.93.10.4793;
RA Riechmann J.L., Krizek B.A., Meyerowitz E.M.;
RT "Dimerization specificity of Arabidopsis MADS domain homeotic proteins
RT APETALA1, APETALA3, PISTILLATA, and AGAMOUS.";
RL Proc. Natl. Acad. Sci. U.S.A. 93:4793-4798(1996).
RN [11]
RP INDUCTION.
RX PubMed=9783581; DOI=10.1038/26903;
RA Parcy F., Nilsson O., Busch M.A., Lee I., Weigel D.;
RT "A genetic framework for floral patterning.";
RL Nature 395:561-566(1998).
RN [12]
RP FUNCTION.
RX PubMed=11283333; DOI=10.2307/3871337;
RA Ng M., Yanofsky M.F.;
RT "Activation of the Arabidopsis B class homeotic genes by APETALA1.";
RL Plant Cell 13:739-753(2001).
RN [13]
RP CHARACTERIZATION.
RX PubMed=11206550; DOI=10.1038/35054083;
RA Honma T., Goto K.;
RT "Complexes of MADS-box proteins are sufficient to convert leaves into
RT floral organs.";
RL Nature 409:525-529(2001).
RN [14]
RP INTERACTION WITH TT16/AGL32.
RX PubMed=16080001; DOI=10.1007/s00438-005-0010-y;
RA Kaufmann K., Anfang N., Saedler H., Theissen G.;
RT "Mutant analysis, protein-protein interactions and subcellular localization
RT of the Arabidopsis B sister (ABS) protein.";
RL Mol. Genet. Genomics 274:103-118(2005).
RN [15]
RP INTERACTION WITH AGL15 AND AGL16.
RX PubMed=15805477; DOI=10.1105/tpc.105.031831;
RA de Folter S., Immink R.G.H., Kieffer M., Parenicova L., Henz S.R.,
RA Weigel D., Busscher M., Kooiker M., Colombo L., Kater M.M., Davies B.,
RA Angenent G.C.;
RT "Comprehensive interaction map of the Arabidopsis MADS Box transcription
RT factors.";
RL Plant Cell 17:1424-1433(2005).
RN [16]
RP INTERACTION WITH AGL24; SVP AND SEU-LUG.
RX PubMed=16679456; DOI=10.1105/tpc.106.041798;
RA Gregis V., Sessa A., Colombo L., Kater M.M.;
RT "AGL24, SHORT VEGETATIVE PHASE, and APETALA1 redundantly control AGAMOUS
RT during early stages of flower development in Arabidopsis.";
RL Plant Cell 18:1373-1382(2006).
RN [17]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=17428825; DOI=10.1242/dev.003103;
RA Liu C., Zhou J., Bracha-Drori K., Yalovsky S., Ito T., Yu H.;
RT "Specification of Arabidopsis floral meristem identity by repression of
RT flowering time genes.";
RL Development 134:1901-1910(2007).
RN [18]
RP FUNCTION.
RX PubMed=19656343; DOI=10.1111/j.1365-313x.2009.03985.x;
RA Gregis V., Sessa A., Dorca-Fornell C., Kater M.M.;
RT "The Arabidopsis floral meristem identity genes AP1, AGL24 and SVP directly
RT repress class B and C floral homeotic genes.";
RL Plant J. 60:626-637(2009).
CC -!- FUNCTION: Transcription factor that promotes early floral meristem
CC identity in synergy with LEAFY. Is required subsequently for the
CC transition of an inflorescence meristem into a floral meristem. Is
CC indispensable for normal development of sepals and petals in flowers.
CC Regulates positively the B class homeotic proteins APETALA3 and
CC PISTILLATA with the cooperation of LEAFY and UFO. Interacts with
CC SEPALLATA3 or AP3/PI heterodimer to form complexes that could be
CC involved in genes regulation during floral meristem development.
CC Regulates positively AGAMOUS in cooperation with LEAFY. Displays a
CC redundant function with CAULIFLOWER in the up-regulation of LEAFY.
CC Together with AGL24 and SVP, controls the identity of the floral
CC meristem and regulates expression of class B, C and E genes. Represses
CC flowering time genes AGL24, SVP and SOC1 in emerging floral meristems.
CC {ECO:0000269|PubMed:11283333, ECO:0000269|PubMed:17428825,
CC ECO:0000269|PubMed:17794879, ECO:0000269|PubMed:19656343,
CC ECO:0000269|Ref.8}.
CC -!- SUBUNIT: Homodimer capable of binding to CArG-box sequences.
CC Heterodimer with SEP3, AP1 and SVP. Binds AP3/PI to form a ternary
CC complex. Interacts with the SEU-LUG corepressor complex when complexed
CC to AGL24 or SVP. Interacts with AGL15 and AGL16 (PubMed:15805477,
CC PubMed:16679456). Interacts with TT16/AGL32 (PubMed:16080001).
CC {ECO:0000269|PubMed:15805477, ECO:0000269|PubMed:16080001,
CC ECO:0000269|PubMed:16679456}.
CC -!- INTERACTION:
CC P35631; Q9SZJ6: AGL21; NbExp=4; IntAct=EBI-592003, EBI-621986;
CC P35631; O82794: AGL24; NbExp=4; IntAct=EBI-592003, EBI-592083;
CC P35631; P29382: SEP1; NbExp=4; IntAct=EBI-592003, EBI-632935;
CC P35631; O22456: SEP3; NbExp=4; IntAct=EBI-592003, EBI-592020;
CC P35631; Q8W234: SEU; NbExp=2; IntAct=EBI-592003, EBI-1771131;
CC P35631; O64645: SOC1; NbExp=5; IntAct=EBI-592003, EBI-592041;
CC P35631; Q9FVC1: SVP; NbExp=5; IntAct=EBI-592003, EBI-592058;
CC P35631; Q93Z00: TCP14; NbExp=3; IntAct=EBI-592003, EBI-4424563;
CC P35631; Q9C9L2: TCP15; NbExp=3; IntAct=EBI-592003, EBI-4426144;
CC P35631; Q8LPR5: TCP4; NbExp=3; IntAct=EBI-592003, EBI-15192325;
CC P35631; Q2NJQ2: AYWB_224; Xeno; NbExp=3; IntAct=EBI-592003, EBI-16100490;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: Expressed in young flower primordia, later becomes
CC localized to sepals and petals.
CC -!- DEVELOPMENTAL STAGE: Expressed at an early stage of floral initiation.
CC -!- INDUCTION: Negatively regulated by TFL1 and by the C class floral
CC homeotic protein AGAMOUS. Positively regulated by CAULIFLOWER.
CC {ECO:0000269|PubMed:9783581}.
CC -!- DISRUPTION PHENOTYPE: Partial conversion of flowers into shoots and a
CC disruption of sepal and petal development.
CC {ECO:0000269|PubMed:17428825}.
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DR EMBL; Z16421; CAA78909.1; -; mRNA.
DR EMBL; AC008262; AAF27070.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE34887.1; -; Genomic_DNA.
DR EMBL; BT004113; AAO42136.1; -; mRNA.
DR EMBL; BT004951; AAO50484.1; -; mRNA.
DR EMBL; AK175933; BAD43696.1; -; mRNA.
DR EMBL; AY087956; AAM65504.1; -; mRNA.
DR EMBL; AF466771; AAM28447.1; -; Genomic_DNA.
DR EMBL; AF466772; AAM28448.1; -; Genomic_DNA.
DR EMBL; AF466773; AAM28449.1; -; Genomic_DNA.
DR EMBL; AF466774; AAM28450.1; -; Genomic_DNA.
DR EMBL; AF466775; AAM28451.1; -; Genomic_DNA.
DR EMBL; AF466776; AAM28452.1; -; Genomic_DNA.
DR EMBL; AF466777; AAM28453.1; -; Genomic_DNA.
DR EMBL; AF466778; AAM28454.1; -; Genomic_DNA.
DR EMBL; AF466779; AAM28455.1; -; Genomic_DNA.
DR EMBL; AF466780; AAM28456.1; -; Genomic_DNA.
DR EMBL; AF466781; AAM28457.1; -; Genomic_DNA.
DR EMBL; AF466782; AAM28458.1; -; Genomic_DNA.
DR EMBL; AF466783; AAM28459.1; -; Genomic_DNA.
DR EMBL; AF466784; AAM28460.1; -; Genomic_DNA.
DR EMBL; AF466785; AAM28461.1; -; Genomic_DNA.
DR PIR; S27109; S27109.
DR RefSeq; NP_177074.1; NM_105581.3.
DR AlphaFoldDB; P35631; -.
DR SMR; P35631; -.
DR BioGRID; 28465; 57.
DR DIP; DIP-33763N; -.
DR IntAct; P35631; 42.
DR STRING; 3702.AT1G69120.1; -.
DR PaxDb; P35631; -.
DR PRIDE; P35631; -.
DR ProteomicsDB; 244444; -.
DR EnsemblPlants; AT1G69120.1; AT1G69120.1; AT1G69120.
DR GeneID; 843244; -.
DR Gramene; AT1G69120.1; AT1G69120.1; AT1G69120.
DR KEGG; ath:AT1G69120; -.
DR Araport; AT1G69120; -.
DR TAIR; locus:2033273; AT1G69120.
DR eggNOG; KOG0014; Eukaryota.
DR HOGENOM; CLU_053053_0_2_1; -.
DR InParanoid; P35631; -.
DR OrthoDB; 1178730at2759; -.
DR PhylomeDB; P35631; -.
DR PRO; PR:P35631; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; P35631; baseline and differential.
DR Genevisible; P35631; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003677; F:DNA binding; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046982; F:protein heterodimerization activity; IPI:TAIR.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0010582; P:floral meristem determinacy; IGI:TAIR.
DR GO; GO:0009908; P:flower development; IMP:TAIR.
DR GO; GO:0009933; P:meristem structural organization; IMP:TAIR.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:InterPro.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:TAIR.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00265; MADS_MEF2_like; 1.
DR Gene3D; 3.40.1810.10; -; 1.
DR InterPro; IPR033896; MADS_MEF2-like.
DR InterPro; IPR002487; TF_Kbox.
DR InterPro; IPR002100; TF_MADSbox.
DR InterPro; IPR036879; TF_MADSbox_sf.
DR Pfam; PF01486; K-box; 1.
DR Pfam; PF00319; SRF-TF; 1.
DR PRINTS; PR00404; MADSDOMAIN.
DR SMART; SM00432; MADS; 1.
DR SUPFAM; SSF55455; SSF55455; 1.
DR PROSITE; PS51297; K_BOX; 1.
DR PROSITE; PS00350; MADS_BOX_1; 1.
DR PROSITE; PS50066; MADS_BOX_2; 1.
PE 1: Evidence at protein level;
KW Activator; Coiled coil; Developmental protein; Differentiation;
KW DNA-binding; Flowering; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..256
FT /note="Floral homeotic protein APETALA 1"
FT /id="PRO_0000199451"
FT DOMAIN 1..61
FT /note="MADS-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00251"
FT DOMAIN 88..178
FT /note="K-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00629"
FT COILED 88..185
FT /evidence="ECO:0000255"
FT VARIANT 85
FT /note="S -> P (in strain: cv. Chi-1)"
FT VARIANT 93
FT /note="M -> T (in strain: cv. Bla-1)"
FT VARIANT 99
FT /note="K -> R (in strain: cv. Chi-1)"
FT VARIANT 100
FT /note="A -> T (in strain: cv. Landsberg erecta)"
FT VARIANT 120
FT /note="M -> V (in strain: cv. Jl-1)"
FT VARIANT 125
FT /note="L -> P (in strain: cv. Bla-1)"
FT VARIANT 166
FT /note="S -> G (in strain: cv. Jl-1)"
FT VARIANT 212
FT /note="L -> P (in strain: cv. Chi-1)"
FT VARIANT 233
FT /note="A -> V (in strain: cv. Co-1)"
FT CONFLICT 139
FT /note="I -> M (in Ref. 5; BAD43696)"
FT /evidence="ECO:0000305"
FT CONFLICT 184
FT /note="E -> G (in Ref. 7; AAM28458)"
FT /evidence="ECO:0000305"
FT CONFLICT 189
FT /note="Q -> H (in Ref. 6; AAM65504)"
FT /evidence="ECO:0000305"
FT CONFLICT 236
FT /note="Missing (in Ref. 1; CAA78909)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 256 AA; 30182 MW; 01E26DE18CE3478C CRC64;
MGRGRVQLKR IENKINRQVT FSKRRAGLLK KAHEISVLCD AEVALVVFSH KGKLFEYSTD
SCMEKILERY ERYSYAERQL IAPESDVNTN WSMEYNRLKA KIELLERNQR HYLGEDLQAM
SPKELQNLEQ QLDTALKHIR TRKNQLMYES INELQKKEKA IQEQNSMLSK QIKEREKILR
AQQEQWDQQN QGHNMPPPLP PQQHQIQHPY MLSHQPSPFL NMGGLYQEDD PMAMRRNDLE
LTLEPVYNCN LGCFAA
