AP21_ORYSJ
ID AP21_ORYSJ Reviewed; 512 AA.
AC Q2TQ34; B9FM73;
DT 16-JAN-2019, integrated into UniProtKB/Swiss-Prot.
DT 24-JAN-2006, sequence version 1.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=APETALA2-like protein 1 {ECO:0000303|PubMed:28066457};
DE AltName: Full=APETALA2-like protein 23 {ECO:0000303|Ref.1};
DE AltName: Full=Protein RICE STARCH REGULATOR 1 {ECO:0000303|PubMed:20713616};
DE AltName: Full=Protein TARGET OF EAT 1 {ECO:0000303|PubMed:26631749};
DE Short=OsTOE1 {ECO:0000303|PubMed:26631749};
GN Name=AP2-1 {ECO:0000303|PubMed:28066457};
GN Synonyms=AP2D23 {ECO:0000303|Ref.1}, RSR1 {ECO:0000303|PubMed:20713616},
GN TOE1 {ECO:0000303|PubMed:26631749};
GN OrderedLocusNames=LOC_Os05g03040 {ECO:0000305}, Os05g0121600 {ECO:0000305};
GN ORFNames=OsJ_16937 {ECO:0000312|EMBL:EEE62150.1},
GN OSNPB_050121600 {ECO:0000312|EMBL:BAS92025.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=cv. Dongjin;
RA Lee J., Lee D.Y., An G.;
RT "The AP2 subfamily genes in rice.";
RL Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=20713616; DOI=10.1104/pp.110.159517;
RA Fu F.-F., Xue H.-W.;
RT "Coexpression analysis identifies Rice Starch Regulator1, a rice AP2/EREBP
RT family transcription factor, as a novel rice starch biosynthesis
RT regulator.";
RL Plant Physiol. 154:927-938(2010).
RN [6]
RP DEVELOPMENTAL STAGE, AND TISSUE SPECIFICITY.
RX PubMed=25817414; DOI=10.1016/j.jplph.2015.03.003;
RA Sekhar S., Panda B.B., Mohapatra T., Das K., Shaw B.P., Kariali E.,
RA Mohapatra P.K.;
RT "Spikelet-specific variation in ethylene production and constitutive
RT expression of ethylene receptors and signal transducers during grain
RT filling of compact- and lax-panicle rice (Oryza sativa) cultivars.";
RL J. Plant Physiol. 179:21-34(2015).
RN [7]
RP FUNCTION, REPRESSION BY MIR172, AND INTERACTION WITH TPR2/ASP1.
RX PubMed=26631749; DOI=10.1073/pnas.1521949112;
RA Wang L., Sun S., Jin J., Fu D., Yang X., Weng X., Xu C., Li X., Xiao J.,
RA Zhang Q.;
RT "Coordinated regulation of vegetative and reproductive branching in rice.";
RL Proc. Natl. Acad. Sci. U.S.A. 112:15504-15509(2015).
RN [8]
RP FUNCTION, REPRESSION BY MIR172, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Zhonghua 11;
RX PubMed=28066457; DOI=10.3389/fpls.2016.01891;
RA Dai Z., Wang J., Zhu M., Miao X., Shi Z.;
RT "OsMADS1 represses microRNA172 in elongation of palea/lemma development in
RT rice.";
RL Front. Plant Sci. 7:1891-1891(2016).
RN [9]
RP REGULATION BY SRT1.
RX PubMed=27181944; DOI=10.1016/j.plantsci.2016.04.004;
RA Zhang H., Lu Y., Zhao Y., Zhou D.-X.;
RT "OsSRT1 is involved in rice seed development through regulation of starch
RT metabolism gene expression.";
RL Plant Sci. 248:28-36(2016).
CC -!- FUNCTION: Probable transcription factor (By similarity). Involved in
CC spikelet transition (Probable). Regulator of starch biosynthesis
CC especially during seed development (e.g. endosperm starch granules);
CC represses the expression of type I starch synthesis genes
CC (PubMed:20713616). Prevents lemma and palea elongation as well as grain
CC growth (PubMed:28066457). {ECO:0000250|UniProtKB:P47927,
CC ECO:0000269|PubMed:20713616, ECO:0000269|PubMed:28066457,
CC ECO:0000305|PubMed:26631749}.
CC -!- SUBUNIT: May form homodimer (By similarity). Interacts with TPR2/ASP1
CC (PubMed:26631749). {ECO:0000250|UniProtKB:P47927,
CC ECO:0000269|PubMed:26631749}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00366}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q2TQ34-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q2TQ34-2; Sequence=VSP_059993;
CC -!- TISSUE SPECIFICITY: Mostly expressed in roots, seedlings and leaves
CC and, to a lower extent, in stems, panicles and developing seeds
CC (PubMed:20713616). Present in spikelets (PubMed:25817414).
CC {ECO:0000269|PubMed:20713616, ECO:0000269|PubMed:25817414}.
CC -!- DEVELOPMENTAL STAGE: Accumulates progressively in panicles. Present at
CC low levels in developing seeds, with a progressive decrease during
CC development (PubMed:20713616). In spikelets, fades out progressively
CC after anthesis (PubMed:25817414). {ECO:0000269|PubMed:20713616,
CC ECO:0000269|PubMed:25817414}.
CC -!- INDUCTION: Target of miR172 microRNA mediated cleavage, particularly
CC during floral organ development (Probable). Repressed by SRT1 via
CC epigenetic histone H3K9 acetylation (H3K9ac) regulation
CC (PubMed:27181944). {ECO:0000269|PubMed:27181944,
CC ECO:0000305|PubMed:26631749, ECO:0000305|PubMed:28066457}.
CC -!- DISRUPTION PHENOTYPE: Larger seed size and increased seed mass and
CC yield. Enhanced expression of starch synthesis genes in seeds leading
CC to increased seed amylose content and altered fine structure of
CC amylopectin. Decreased proportion of A chains results in abnormal round
CC and loosely packed starch granules but reduced gelatinization
CC temperature. {ECO:0000269|PubMed:20713616}.
CC -!- SIMILARITY: Belongs to the AP2/ERF transcription factor family. AP2
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EEE62150.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY685117; AAW78371.1; -; mRNA.
DR EMBL; AP014961; BAS92025.1; -; Genomic_DNA.
DR EMBL; CM000142; EEE62150.1; ALT_SEQ; Genomic_DNA.
DR RefSeq; XP_015639329.1; XM_015783843.1. [Q2TQ34-1]
DR AlphaFoldDB; Q2TQ34; -.
DR SMR; Q2TQ34; -.
DR STRING; 4530.OS05T0121600-01; -.
DR PaxDb; Q2TQ34; -.
DR PRIDE; Q2TQ34; -.
DR EnsemblPlants; Os05t0121600-01; Os05t0121600-01; Os05g0121600. [Q2TQ34-1]
DR GeneID; 4337654; -.
DR Gramene; Os05t0121600-01; Os05t0121600-01; Os05g0121600. [Q2TQ34-1]
DR KEGG; osa:4337654; -.
DR eggNOG; ENOG502R1D7; Eukaryota.
DR HOGENOM; CLU_035462_0_0_1; -.
DR OMA; KASCNSN; -.
DR OrthoDB; 1127651at2759; -.
DR PlantReactome; R-OSA-9608931; Floral bracts development.
DR Proteomes; UP000007752; Chromosome 5.
DR Proteomes; UP000059680; Chromosome 5.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IMP:UniProtKB.
DR GO; GO:2000014; P:regulation of endosperm development; IMP:UniProtKB.
DR GO; GO:0009909; P:regulation of flower development; IMP:UniProtKB.
DR GO; GO:0080050; P:regulation of seed development; IMP:UniProtKB.
DR GO; GO:0010581; P:regulation of starch biosynthetic process; IMP:UniProtKB.
DR CDD; cd00018; AP2; 2.
DR Gene3D; 3.30.730.10; -; 2.
DR InterPro; IPR001471; AP2/ERF_dom.
DR InterPro; IPR036955; AP2/ERF_dom_sf.
DR InterPro; IPR016177; DNA-bd_dom_sf.
DR Pfam; PF00847; AP2; 2.
DR PRINTS; PR00367; ETHRSPELEMNT.
DR SMART; SM00380; AP2; 2.
DR SUPFAM; SSF54171; SSF54171; 2.
DR PROSITE; PS51032; AP2_ERF; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Nucleus; Reference proteome; Repeat;
KW Repressor; Transcription; Transcription regulation.
FT CHAIN 1..512
FT /note="APETALA2-like protein 1"
FT /id="PRO_0000445987"
FT DNA_BIND 171..227
FT /note="AP2/ERF 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00366"
FT DNA_BIND 263..320
FT /note="AP2/ERF 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00366"
FT REGION 1..100
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 119..174
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 348..367
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 401..433
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 460..512
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 159..168
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT MOTIF 341..345
FT /note="EAR"
FT /evidence="ECO:0000250|UniProtKB:P47927"
FT COMPBIAS 135..156
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 460..504
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 380
FT /note="K -> KASCNS (in isoform 2)"
FT /id="VSP_059993"
SQ SEQUENCE 512 AA; 55608 MW; 6D92981D95DFD572 CRC64;
MELDLNNVAE GVVEKHETAA RSDSGTSESS VLNGEASGAA IAPAEEGSSS TPPSPPPPPA
AVLEFSILRS SASASGENDA DDDEEEEATP SPPPHHQHQQ LLVTRELFPS AAPSPQHWAE
LGFLRPDPPR PHPDIRILAH APPPAPPPPP PQPQPQAAKK SRRGPRSRSS QYRGVTFYRR
TGRWESHIWD CGKQVYLGGF DTAHAAARAY DRAAIKFRGV EADINFNLSD YEEDMRQMKS
LSKEEFVHVL RRQSTGFSRG SSKYRGVTLH KCGRWEARMG QFLGKKYIYL GLFDSEVEAA
RAYDKAAIKC NGREAVTNFE PSTYDGELPT DAAAQGADVD LNLRISQPAA SQQSPKRDSG
SLGLQIHHGS FEGSEFKRAK NDAAPSELAS RPHRFPLLTE HPPIWTAQPH PLFPNNEDAS
RSSDQKRKPS EGVAVPSWAW KQVSHHHPAP PHTLPLPFFS SSSSSPSSSS AAASSGFSKA
ATTAAAAQHT ATLRFDPTAP SSSSSSRHHH HH
