HRD1_ORYSJ
ID HRD1_ORYSJ Reviewed; 500 AA.
AC Q5Z880; A0A0P0WVN5;
DT 26-NOV-2014, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=ERAD-associated E3 ubiquitin-protein ligase HRD1 {ECO:0000305};
DE EC=2.3.2.27 {ECO:0000305};
DE AltName: Full=RING-type E3 ubiquitin transferase HRD1 {ECO:0000305};
GN Name=HRD1 {ECO:0000305};
GN OrderedLocusNames=Os06g0301000 {ECO:0000312|EMBL:BAF19368.1},
GN LOC_Os06g19680 {ECO:0000305};
GN ORFNames=OJ1217_C01.4 {ECO:0000312|EMBL:BAD53976.1},
GN P0501G08.42 {ECO:0000312|EMBL:BAD53494.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947 {ECO:0000312|Proteomes:UP000059680};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND G.
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- FUNCTION: Probable component of the HRD1 ubiquitin ligase complex that
CC mediates the rapid degradation of misfolded endoplasmic reticulum (ER)
CC proteins, a process called ER-associated degradation (ERAD).
CC {ECO:0000250|UniProtKB:Q9LW77}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000305};
CC -!- PATHWAY: Protein modification; protein ubiquitination. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000305};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the HRD1 family. {ECO:0000305}.
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DR EMBL; AP003524; BAD53494.1; -; Genomic_DNA.
DR EMBL; AP004007; BAD53976.1; -; Genomic_DNA.
DR EMBL; AP008212; BAF19368.1; -; Genomic_DNA.
DR EMBL; AP014962; BAS97382.1; -; Genomic_DNA.
DR EMBL; AK073960; BAG93732.1; -; mRNA.
DR RefSeq; XP_015644046.1; XM_015788560.1.
DR AlphaFoldDB; Q5Z880; -.
DR SMR; Q5Z880; -.
DR STRING; 4530.OS06T0301000-01; -.
DR PaxDb; Q5Z880; -.
DR PRIDE; Q5Z880; -.
DR EnsemblPlants; Os06t0301000-01; Os06t0301000-01; Os06g0301000.
DR GeneID; 4340826; -.
DR Gramene; Os06t0301000-01; Os06t0301000-01; Os06g0301000.
DR KEGG; osa:4340826; -.
DR eggNOG; KOG0802; Eukaryota.
DR HOGENOM; CLU_009169_4_1_1; -.
DR InParanoid; Q5Z880; -.
DR OMA; MEFTMLL; -.
DR OrthoDB; 897451at2759; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000000763; Chromosome 6.
DR Proteomes; UP000059680; Chromosome 6.
DR Genevisible; Q5Z880; OS.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR GO; GO:0044260; P:cellular macromolecule metabolic process; IEA:UniProt.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR Pfam; PF13639; zf-RING_2; 1.
DR SMART; SM00184; RING; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Membrane; Metal-binding; Reference proteome;
KW Transferase; Transmembrane; Transmembrane helix; Ubl conjugation pathway;
KW Zinc; Zinc-finger.
FT CHAIN 1..500
FT /note="ERAD-associated E3 ubiquitin-protein ligase HRD1"
FT /id="PRO_0000431272"
FT TOPO_DOM 1..3
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 4..24
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 25..40
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 41..61
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 62..98
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 99..119
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 120..135
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 136..156
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 157..170
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 171..191
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 192..225
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 226..246
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 247..500
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT ZN_FING 292..330
FT /note="RING-type; atypical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00175"
FT REGION 337..375
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 398..438
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 398..431
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 500 AA; 56262 MW; 1C42744325E09277 CRC64;
MIRLQTYAAF SLMATATAVY YAFSSREQFY PAMVYLSTSK ICFVLLLNTG LVAMCVAWQL
VKRLFLGTLR EAEVERLNEQ AWREVVEILF AVTIFRQDFS VSFLAMVAAL LLVKALHWLA
QKRVEYIETT PSVPMLSHAR IVSFMLFLLV VDCLFLSNSL RSLIHKREAS VAIFFSFEYM
ILATSTVSTF VKYIFYVSDM LMEGQWEKKA VYTFYLELIS DLVHLSLYML FFIAIFLNYG
VPLHLIRELY ETFRNFRIRI ADYVRYRKIT SNMNERFPDA TADELNASDA TCIICREEMT
TAKKLLCGHL FHVHCLRSWL ERQHTCPTCR APILPPDNGR TAARPHGVHP GVQPVPGNGT
PGSERAAGEN ISRRQAKLEA AASAASLYGR SFAYPPANNL NRYSTPPQST SNGPQSGEAS
TSNQSPKGHA TADPSAPTFY ARGAVSSVTT TRELESSLQK AYENAIKSQI EMLQIQLQMF
QHGATSSATN NENGEHTKSD
