HRDA_STRCO
ID HRDA_STRCO Reviewed; 396 AA.
AC P18182; Q9L2F1;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 18-OCT-2001, sequence version 2.
DT 25-MAY-2022, entry version 140.
DE RecName: Full=RNA polymerase principal sigma factor HrdA;
GN Name=hrdA; OrderedLocusNames=SCO2465; ORFNames=SC7A8.04c;
OS Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145).
OC Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC Streptomyces; Streptomyces albidoflavus group.
OX NCBI_TaxID=100226;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=A3(2) / NRRL B-16638;
RX PubMed=1944221; DOI=10.1007/bf00267453;
RA Tanaka K., Shiina T., Takahashi H.;
RT "Nucleotide sequence of genes hrdA, hrdC, and hrdD from Streptomyces
RT coelicolor A3(2) having similarity to rpoD genes.";
RL Mol. Gen. Genet. 229:334-340(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-471 / A3(2) / M145;
RX PubMed=12000953; DOI=10.1038/417141a;
RA Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L.,
RA Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D.,
RA Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A.,
RA Fraser A., Goble A., Hidalgo J., Hornsby T., Howarth S., Huang C.-H.,
RA Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E.,
RA Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D.,
RA Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A.,
RA Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A.;
RT "Complete genome sequence of the model actinomycete Streptomyces coelicolor
RT A3(2).";
RL Nature 417:141-147(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 191-242.
RC STRAIN=A3(2) / NRRL B-16638;
RX PubMed=3194753; DOI=10.1126/science.3194753;
RA Tanaka K., Shiina T., Takahashi H.;
RT "Multiple principal sigma factor homologs in eubacteria: identification of
RT the 'rpoD box'.";
RL Science 242:1040-1042(1988).
RN [4]
RP SUBUNIT.
RC STRAIN=ATCC BAA-471 / A3(2) / M145;
RX PubMed=23605043; DOI=10.1093/nar/gkt277;
RA Tabib-Salazar A., Liu B., Doughty P., Lewis R.A., Ghosh S., Parsy M.L.,
RA Simpson P.J., O'Dwyer K., Matthews S.J., Paget M.S.;
RT "The actinobacterial transcription factor RbpA binds to the principal sigma
RT subunit of RNA polymerase.";
RL Nucleic Acids Res. 41:5679-5691(2013).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released.
CC -!- SUBUNIT: Interacts transiently with the RNA polymerase catalytic core
CC (By similarity). Interacts with RNA polymerase-binding protein RbpA.
CC {ECO:0000250, ECO:0000269|PubMed:23605043}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. {ECO:0000305}.
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DR EMBL; X52980; CAA37172.1; -; Genomic_DNA.
DR EMBL; AL939112; CAB69753.1; -; Genomic_DNA.
DR PIR; S17929; S17929.
DR RefSeq; NP_626707.1; NC_003888.3.
DR RefSeq; WP_003976339.1; NZ_VNID01000001.1.
DR AlphaFoldDB; P18182; -.
DR SMR; P18182; -.
DR STRING; 100226.SCO2465; -.
DR GeneID; 1097899; -.
DR KEGG; sco:SCO2465; -.
DR PATRIC; fig|100226.15.peg.2506; -.
DR eggNOG; COG0568; Bacteria.
DR HOGENOM; CLU_014793_3_6_11; -.
DR InParanoid; P18182; -.
DR OMA; YWWISEE; -.
DR PhylomeDB; P18182; -.
DR Proteomes; UP000001973; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR Gene3D; 1.10.10.10; -; 2.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR000943; RNA_pol_sigma70.
DR InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR007624; RNA_pol_sigma70_r3.
DR InterPro; IPR007630; RNA_pol_sigma70_r4.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF00140; Sigma70_r1_2; 1.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF04539; Sigma70_r3; 1.
DR Pfam; PF04545; Sigma70_r4; 1.
DR PRINTS; PR00046; SIGMA70FCT.
DR SUPFAM; SSF88659; SSF88659; 2.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR PROSITE; PS00715; SIGMA70_1; 1.
DR PROSITE; PS00716; SIGMA70_2; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Reference proteome; Sigma factor; Transcription;
KW Transcription regulation.
FT CHAIN 1..396
FT /note="RNA polymerase principal sigma factor HrdA"
FT /id="PRO_0000093990"
FT DNA_BIND 357..376
FT /note="H-T-H motif"
FT /evidence="ECO:0000250"
FT REGION 1..96
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 187..200
FT /note="Polymerase core binding"
FT COMPBIAS 29..43
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 75..91
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 211
FT /note="F -> I (in Ref. 3; no nucleotide entry)"
FT /evidence="ECO:0000305"
FT CONFLICT 364
FT /note="F -> G (in Ref. 1; CAA37172)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 396 AA; 43837 MW; EB711BA589E58FCA CRC64;
MRGGQRRASR LRPPTYRRRP PPAASILEVA PVQTQTLTQT DTAAGGAEPD AERGVLLAMP
AQPGAGAALP HPGAPVDVPE HPEPPPPTRT ESGGPSSDLF RQYLREIGRI PLLSAAEEVD
LARRVEAGLF AEEKLRCSPG LDDRLALDLD RLVVLGRLAK RRLIEANLRL VVSVAKRYVG
RGLTMLDLVQ EGNLGLIRAV EKFDYARGYK FSTYATWWIR QAMSRALADQ ARTIRVPVHV
VELINRVVRV QRRMLQERGC EPTPQEVAAH LDLAPERVGE VLRLAQEPVS LHAPVGEEDD
VALGDLIEDG DAASPVESAA FLLLRQHLEA VLSTLGERER KVVQLRYGLA DGRPRTLEEI
GRLFGVTRER IRQIESKTLS KLRDHAYADQ LRGYLD
