ID   HRDE2_CAEEL             Reviewed;         305 AA.
AC   Q22058;
DT   27-SEP-2017, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 105.
DE   RecName: Full=Protein hrde-2 {ECO:0000305};
DE   AltName: Full=Heritable RNAi deficient protein 2 {ECO:0000312|WormBase:T01C3.9};
GN   Name=hrde-2 {ECO:0000303|PubMed:28533440};
GN   ORFNames=T01C3.9 {ECO:0000312|WormBase:T01C3.9};
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN   [1] {ECO:0000312|Proteomes:UP000001940}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
RN   [2] {ECO:0000305}
RP   FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP   PHENOTYPE.
RX   PubMed=28533440; DOI=10.1534/genetics.116.198812;
RA   Spracklin G., Fields B., Wan G., Vijayendran D., Wallig A., Shukla A.,
RA   Kennedy S.;
RT   "Identification and characterization of Caenorhabditis elegans RNAi
RT   inheritance machinery.";
RL   Genetics 206:1403-1416(2017).
CC   -!- FUNCTION: Plays a role in germline RNA interference (RNAi), and in
CC       particular is required for piwi-interacting RNA (piRNA) gene silencing.
CC       Facilitates the binding of the argonaut protein hrde-1 to small
CC       interfering RNAs (siRNAs) targets that are required for
CC       transgenerational epigenetic inheritance and germline immortality.
CC       {ECO:0000269|PubMed:28533440}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:28533440}.
CC   -!- TISSUE SPECIFICITY: Expressed throughout the male and female germline.
CC       {ECO:0000269|PubMed:28533440}.
CC   -!- DISRUPTION PHENOTYPE: Mortal germline (Mrt) phenotype in which there is
CC       a progressive decline in fertility with each generation at 25 degrees
CC       Celsius, and after 2 to 3 generations the brood size is 25% that of
CC       wild-type counterparts. Fertility in these mutants may be restored when
CC       transferred to a 20 degrees Celsius environment. Defective RNAi
CC       inheritance. {ECO:0000269|PubMed:28533440}.
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DR   EMBL; BX284605; CAB01662.1; -; Genomic_DNA.
DR   PIR; T24283; T24283.
DR   RefSeq; NP_506693.1; NM_074292.4.
DR   AlphaFoldDB; Q22058; -.
DR   SMR; Q22058; -.
DR   IntAct; Q22058; 1.
DR   STRING; 6239.T01C3.9; -.
DR   PaxDb; Q22058; -.
DR   EnsemblMetazoa; T01C3.9.1; T01C3.9.1; WBGene00011324.
DR   GeneID; 180001; -.
DR   KEGG; cel:CELE_T01C3.9; -.
DR   UCSC; T01C3.9; c. elegans.
DR   CTD; 180001; -.
DR   WormBase; T01C3.9; CE12924; WBGene00011324; hrde-2.
DR   eggNOG; ENOG502TIP7; Eukaryota.
DR   HOGENOM; CLU_855898_0_0_1; -.
DR   InParanoid; Q22058; -.
DR   OMA; DEGHEYC; -.
DR   OrthoDB; 1810116at2759; -.
DR   PRO; PR:Q22058; -.
DR   Proteomes; UP000001940; Chromosome V.
DR   Bgee; WBGene00011324; Expressed in germ line (C elegans) and 4 other tissues.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0031047; P:gene silencing by RNA; IEA:UniProtKB-KW.
PE   2: Evidence at transcript level;
KW   Nucleus; Reference proteome; RNA-mediated gene silencing.
FT   CHAIN           1..305
FT                   /note="Protein hrde-2"
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000441622"
FT   REGION          211..233
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          267..305
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        211..228
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        270..284
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        285..305
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   305 AA;  34662 MW;  77D836A08CCF5DF3 CRC64;
     MSQNSDTFDS GLQMEVHKMA EADNSSVQQK FDAIRDYIGR KETTIILCEH DDSRKIKLTL
     NKQRMESSMF LTEMPDGGIQ EKTKFHRNGL AVCSYNAFDE VDLPLADSYI FYGLPSNVEA
     FPRMLHGIEK SAHKENKIVF IDIVVSILEK IQIKVVSFEL YNRKCTVPTW VAELVITYCA
     PIEEQQQRIE EIRRTRASVP VAISDAANGS AEMVPSNTTG SSGSPMSTAP VPAAKKEKVQ
     YESIRQIRPN AQGRYFVPAR MINMDIEMSN DEYSPDESEN DENEYDYENA ARYDDGYDEG
     HEYCQ