ID   HRDE4_CAEEL             Reviewed;         545 AA.
AC   H2KZ49; C6S3L8;
DT   27-SEP-2017, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2012, sequence version 1.
DT   03-AUG-2022, entry version 61.
DE   RecName: Full=Protein hrde-4 {ECO:0000305};
DE   AltName: Full=Heritable RNAi deficient protein 4 {ECO:0000312|WormBase:T10B11.7a};
GN   Name=hrde-4 {ECO:0000303|PubMed:28533440};
GN   ORFNames=T10B11.7 {ECO:0000312|WormBase:T10B11.7a};
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN   [1] {ECO:0000312|Proteomes:UP000001940}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
RN   [2] {ECO:0000305}
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=28533440; DOI=10.1534/genetics.116.198812;
RA   Spracklin G., Fields B., Wan G., Vijayendran D., Wallig A., Shukla A.,
RA   Kennedy S.;
RT   "Identification and characterization of Caenorhabditis elegans RNAi
RT   inheritance machinery.";
RL   Genetics 206:1403-1416(2017).
CC   -!- FUNCTION: May play a role in transgenerational epigenetic inheritance.
CC       {ECO:0000305|PubMed:28533440}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=a {ECO:0000312|WormBase:T10B11.7a};
CC         IsoId=H2KZ49-1; Sequence=Displayed;
CC       Name=b {ECO:0000312|WormBase:T10B11.7b};
CC         IsoId=H2KZ49-2; Sequence=VSP_059070;
CC   -!- DISRUPTION PHENOTYPE: Mortal germline (Mrt) phenotype in which there is
CC       a progressive decline in fertility with each generation at 25 degrees
CC       Celsius. {ECO:0000269|PubMed:28533440}.
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DR   EMBL; BX284601; CCD66246.1; -; Genomic_DNA.
DR   EMBL; BX284601; CCD66247.1; -; Genomic_DNA.
DR   RefSeq; NP_001040682.2; NM_001047217.2. [H2KZ49-1]
DR   RefSeq; NP_001040683.2; NM_001047218.2.
DR   AlphaFoldDB; H2KZ49; -.
DR   IntAct; H2KZ49; 1.
DR   STRING; 6239.T10B11.7a; -.
DR   PaxDb; H2KZ49; -.
DR   EnsemblMetazoa; T10B11.7a.1; T10B11.7a.1; WBGene00020403. [H2KZ49-1]
DR   EnsemblMetazoa; T10B11.7b.1; T10B11.7b.1; WBGene00020403. [H2KZ49-2]
DR   GeneID; 188371; -.
DR   KEGG; cel:CELE_T10B11.7; -.
DR   CTD; 188371; -.
DR   WormBase; T10B11.7a; CE43751; WBGene00020403; hrde-4. [H2KZ49-1]
DR   WormBase; T10B11.7b; CE43803; WBGene00020403; hrde-4. [H2KZ49-2]
DR   eggNOG; ENOG502TKB0; Eukaryota.
DR   HOGENOM; CLU_536639_0_0_1; -.
DR   OrthoDB; 1885114at2759; -.
DR   SignaLink; H2KZ49; -.
DR   PRO; PR:H2KZ49; -.
DR   Proteomes; UP000001940; Chromosome I.
DR   Bgee; WBGene00020403; Expressed in embryo and 4 other tissues.
DR   GO; GO:0031047; P:gene silencing by RNA; IEA:UniProtKB-KW.
PE   4: Predicted;
KW   Alternative splicing; Coiled coil; Reference proteome;
KW   RNA-mediated gene silencing.
FT   CHAIN           1..545
FT                   /note="Protein hrde-4"
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000441623"
FT   REGION          108..150
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          236..290
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          430..461
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          493..545
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          325..354
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        120..136
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        236..274
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        430..452
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        493..525
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        529..545
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VAR_SEQ         1..37
FT                   /note="Missing (in isoform b)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_059070"
SQ   SEQUENCE   545 AA;  62718 MW;  A78155B0CD24C0BA CRC64;
     MNTYFRFEWI NGEENPCEYN LWRMFQIEPK PKSGANFMKP NLPNTENFST ASSSKIRQLS
     EQPSCSNSYK KLRHEMGSDN SNIDDPLDDY DDRKKKLAKK KAAFKAKKGY TTGQNKSDNS
     FEFEVEKSMD RKEPENGDEE ESTEDDYRHA YSSVEKSLRK ISLLPIQRPS HHTFCVFCSE
     SYPPVEFHFC IFMTAAQKEV NQSVNEFLGK ADTINKKMLE SLKKFIHTSK VDTIAKHSRM
     ENTSMKKNSD SPKKLKRPST DPNPISKRFR HISDESTSSS SLAKNKSRSV TPDALPFAMI
     DSQEHRTQSG RVVKRPNLFC HKEVQIRKET VQKEGLKKKR VNKEKEVEQE STILLGISES
     ERKRNRNMIR RLLANGFELP GDDYVLPPLP ADKSTWTREQ HKMDIYGPIY CALQSFKPAY
     TTDMTSEFLE DDGDTEFKSE NSESDSDSED EQKVENDVAS SADDIEFIEI VEAERKMDQN
     LAQQVRSSFV TTAENTENNM LMEDDSSEQG SSKFNRNASR RSTENIEFIT IDDDDDDDND
     IEIIA