HRI1_YEAST
ID HRI1_YEAST Reviewed; 244 AA.
AC Q05905; D6VYU5;
DT 11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Protein HRI1;
DE AltName: Full=HRR25-interacting protein 1;
GN Name=HRI1; OrderedLocusNames=YLR301W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169871;
RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA Zollner A., Hani J., Hoheisel J.D.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL Nature 387:87-90(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP INTERACTION WITH SEC72.
RX PubMed=12518317; DOI=10.1002/yea.954;
RA Willer M., Jermy A.J., Young B.P., Stirling C.J.;
RT "Identification of novel protein-protein interactions at the cytosolic
RT surface of the Sec63 complex in the yeast ER membrane.";
RL Yeast 20:133-148(2003).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-143, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ADR376;
RX PubMed=17330950; DOI=10.1021/pr060559j;
RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA Elias J.E., Gygi S.P.;
RT "Large-scale phosphorylation analysis of alpha-factor-arrested
RT Saccharomyces cerevisiae.";
RL J. Proteome Res. 6:1190-1197(2007).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-143, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-143, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
RN [10]
RP INTERACTION WITH HRR25.
RX PubMed=21460040; DOI=10.1101/gad.1998811;
RA Fasolo J., Sboner A., Sun M.G., Yu H., Chen R., Sharon D., Kim P.M.,
RA Gerstein M., Snyder M.;
RT "Diverse protein kinase interactions identified by protein microarrays
RT reveal novel connections between cellular processes.";
RL Genes Dev. 25:767-778(2011).
CC -!- FUNCTION: Unknown. Non essential.
CC -!- SUBUNIT: Interacts with HRR25. May interact with SEC72.
CC {ECO:0000269|PubMed:12518317, ECO:0000269|PubMed:21460040}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}. Nucleus
CC {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 15400 molecules/cell in log phase SD
CC medium. {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the HRI1 family. {ECO:0000305}.
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DR EMBL; U17243; AAB67346.1; -; Genomic_DNA.
DR EMBL; AY558217; AAS56543.1; -; Genomic_DNA.
DR EMBL; BK006945; DAA09611.1; -; Genomic_DNA.
DR PIR; S50385; S50385.
DR RefSeq; NP_013404.1; NM_001182189.1.
DR PDB; 3RBY; X-ray; 2.30 A; A/B=1-244.
DR PDBsum; 3RBY; -.
DR AlphaFoldDB; Q05905; -.
DR SMR; Q05905; -.
DR BioGRID; 31565; 89.
DR DIP; DIP-4329N; -.
DR IntAct; Q05905; 2.
DR STRING; 4932.YLR301W; -.
DR iPTMnet; Q05905; -.
DR MaxQB; Q05905; -.
DR PaxDb; Q05905; -.
DR PRIDE; Q05905; -.
DR TopDownProteomics; Q05905; -.
DR EnsemblFungi; YLR301W_mRNA; YLR301W; YLR301W.
DR GeneID; 851008; -.
DR KEGG; sce:YLR301W; -.
DR SGD; S000004292; HRI1.
DR VEuPathDB; FungiDB:YLR301W; -.
DR eggNOG; ENOG502QTYD; Eukaryota.
DR HOGENOM; CLU_097607_0_0_1; -.
DR InParanoid; Q05905; -.
DR OMA; NVPNTHR; -.
DR BioCyc; YEAST:G3O-32392-MON; -.
DR PRO; PR:Q05905; -.
DR Proteomes; UP000002311; Chromosome XII.
DR RNAct; Q05905; protein.
DR GO; GO:0005829; C:cytosol; HDA:SGD.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IPI:SGD.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0006612; P:protein targeting to membrane; IPI:SGD.
DR CDD; cd11692; HRI1_N_like; 1.
DR Gene3D; 2.40.128.320; -; 1.
DR InterPro; IPR031818; Hri1.
DR InterPro; IPR038744; Hri1_N.
DR InterPro; IPR043047; Hri1_N_sf.
DR Pfam; PF16815; HRI1; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..244
FT /note="Protein HRI1"
FT /id="PRO_0000245329"
FT MOD_RES 143
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950,
FT ECO:0007744|PubMed:18407956, ECO:0007744|PubMed:19779198"
FT STRAND 3..14
FT /evidence="ECO:0007829|PDB:3RBY"
FT STRAND 23..26
FT /evidence="ECO:0007829|PDB:3RBY"
FT STRAND 32..40
FT /evidence="ECO:0007829|PDB:3RBY"
FT TURN 43..45
FT /evidence="ECO:0007829|PDB:3RBY"
FT STRAND 46..48
FT /evidence="ECO:0007829|PDB:3RBY"
FT STRAND 50..56
FT /evidence="ECO:0007829|PDB:3RBY"
FT HELIX 58..60
FT /evidence="ECO:0007829|PDB:3RBY"
FT STRAND 61..65
FT /evidence="ECO:0007829|PDB:3RBY"
FT STRAND 67..75
FT /evidence="ECO:0007829|PDB:3RBY"
FT STRAND 78..80
FT /evidence="ECO:0007829|PDB:3RBY"
FT HELIX 81..85
FT /evidence="ECO:0007829|PDB:3RBY"
FT STRAND 95..101
FT /evidence="ECO:0007829|PDB:3RBY"
FT STRAND 107..113
FT /evidence="ECO:0007829|PDB:3RBY"
FT STRAND 123..132
FT /evidence="ECO:0007829|PDB:3RBY"
FT STRAND 136..138
FT /evidence="ECO:0007829|PDB:3RBY"
FT STRAND 155..160
FT /evidence="ECO:0007829|PDB:3RBY"
FT STRAND 165..172
FT /evidence="ECO:0007829|PDB:3RBY"
FT STRAND 175..184
FT /evidence="ECO:0007829|PDB:3RBY"
FT HELIX 188..190
FT /evidence="ECO:0007829|PDB:3RBY"
FT STRAND 191..198
FT /evidence="ECO:0007829|PDB:3RBY"
FT STRAND 204..211
FT /evidence="ECO:0007829|PDB:3RBY"
FT HELIX 214..216
FT /evidence="ECO:0007829|PDB:3RBY"
FT STRAND 229..232
FT /evidence="ECO:0007829|PDB:3RBY"
FT STRAND 235..243
FT /evidence="ECO:0007829|PDB:3RBY"
SQ SEQUENCE 244 AA; 27501 MW; 80D813586A1930BB CRC64;
MPALLKRLLF QVGPHPNERT FTLSSVSTDG HYISLRPFVK PSGDELSFPF EWAFAGTNET
VKANDQGNGV VTQDFNFWLD TNVYLNVPNT HRGEVNTTWK NWDSGCVEET GAVYPFGADK
ESVSFRELWQ PVDPSREDLV IVSPNNEKFS SNARSIVLKV TDEAYDGLVI VIGRWIQGFL
SQKNNNTIEG LNFIRLLEKD SGKSEFLLSY GKEVNKIPQS YENLKKGSTV TSNGLNWEVI
EYHA
