ID   HRK_RAT                 Reviewed;          92 AA.
AC   P62817; P70678;
DT   16-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=Activator of apoptosis harakiri;
DE   AltName: Full=BH3-interacting domain-containing protein 3;
DE   AltName: Full=Neuronal death protein DP5;
GN   Name=Hrk; Synonyms=Bid3, Dp5;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Brain;
RX   PubMed=9228060; DOI=10.1074/jbc.272.30.18842;
RA   Imaizumi K., Tsuda M., Imai Y., Wanaka A., Takagi T., Tohyama M.;
RT   "Molecular cloning of a novel polypeptide, DP5, induced during programmed
RT   neuronal death.";
RL   J. Biol. Chem. 272:18842-18848(1997).
CC   -!- FUNCTION: Promotes apoptosis. {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with BCL2 and BCL2L1. Interacts with C1QBP (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Single-pass membrane
CC       protein {ECO:0000250}. Mitochondrion {ECO:0000250}.
CC   -!- DOMAIN: The BH3 motif is required for the induction of cell death.
CC       {ECO:0000250}.
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DR   EMBL; D83697; BAA12065.1; -; mRNA.
DR   RefSeq; NP_476471.1; NM_057130.4.
DR   AlphaFoldDB; P62817; -.
DR   SMR; P62817; -.
DR   GeneID; 117271; -.
DR   KEGG; rno:117271; -.
DR   UCSC; RGD:70549; rat.
DR   CTD; 8739; -.
DR   RGD; 70549; Hrk.
DR   InParanoid; P62817; -.
DR   OrthoDB; 1642993at2759; -.
DR   PRO; PR:P62817; -.
DR   Proteomes; UP000002494; Unplaced.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005739; C:mitochondrion; ISS:UniProtKB.
DR   GO; GO:0006915; P:apoptotic process; IMP:RGD.
DR   GO; GO:0051365; P:cellular response to potassium ion starvation; IMP:RGD.
DR   GO; GO:0043065; P:positive regulation of apoptotic process; ISS:UniProtKB.
DR   GO; GO:0043525; P:positive regulation of neuron apoptotic process; IMP:RGD.
DR   GO; GO:0031334; P:positive regulation of protein-containing complex assembly; ISO:RGD.
DR   GO; GO:0090200; P:positive regulation of release of cytochrome c from mitochondria; ISO:RGD.
DR   InterPro; IPR017249; Apoptosis_activator_harakiri.
DR   InterPro; IPR020728; Bcl2_BH3_motif_CS.
DR   PANTHER; PTHR15056; PTHR15056; 1.
DR   Pfam; PF15196; Harakiri; 1.
DR   PIRSF; PIRSF037635; BID3; 1.
DR   PROSITE; PS01259; BH3; 1.
PE   3: Inferred from homology;
KW   Apoptosis; Membrane; Mitochondrion; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..92
FT                   /note="Activator of apoptosis harakiri"
FT                   /id="PRO_0000143108"
FT   TRANSMEM        70..88
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   MOTIF           33..47
FT                   /note="BH3"
SQ   SEQUENCE   92 AA;  10078 MW;  95760B9EEA88F1DE CRC64;
     MCPCPRHRGR GPPAVCGCGD ARPGLRWAAA QVTALRLQAL GDELHRRAMR RRARPRDPLP
     ALLPALRARW PWLCAAAQVA ALAAWLLGRR SA