HROB_HUMAN
ID HROB_HUMAN Reviewed; 647 AA.
AC Q8N3J3; A8K7A9; Q9BWM9; Q9HAI1;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Homologous recombination OB-fold protein {ECO:0000303|PubMed:31467087};
GN Name=HROB {ECO:0000312|HGNC:HGNC:28460}; Synonyms=C17orf53;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 4).
RC TISSUE=Embryo, and Neuroepithelioma;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Melanoma;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16625196; DOI=10.1038/nature04689;
RA Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R.,
RA Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A.,
RA Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J.,
RA Chang J.L., Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J.,
RA DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S.,
RA Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E.,
RA Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K.,
RA LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J.,
RA Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A.,
RA Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K.,
RA Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D.,
RA Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A.,
RA Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.;
RT "DNA sequence of human chromosome 17 and analysis of rearrangement in the
RT human lineage.";
RL Nature 440:1045-1049(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), AND VARIANT PRO-126.
RC TISSUE=Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-47, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma, and Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [7]
RP METHYLATION [LARGE SCALE ANALYSIS] AT ARG-285; ARG-295; ARG-329 AND
RP ARG-337, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Colon carcinoma;
RX PubMed=24129315; DOI=10.1074/mcp.o113.027870;
RA Guo A., Gu H., Zhou J., Mulhern D., Wang Y., Lee K.A., Yang V., Aguiar M.,
RA Kornhauser J., Jia X., Ren J., Beausoleil S.A., Silva J.C., Vemulapalli V.,
RA Bedford M.T., Comb M.J.;
RT "Immunoaffinity enrichment and mass spectrometry analysis of protein
RT methylation.";
RL Mol. Cell. Proteomics 13:372-387(2014).
RN [8]
RP FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH MCM8.
RX PubMed=31467087; DOI=10.1101/gad.329508.119;
RA Hustedt N., Saito Y., Zimmermann M., Alvarez-Quilon A., Setiaputra D.,
RA Adam S., McEwan A., Yuan J.Y., Olivieri M., Zhao Y., Kanemaki M.T.,
RA Jurisicova A., Durocher D.;
RT "Control of homologous recombination by the HROB-MCM8-MCM9 pathway.";
RL Genes Dev. 33:1397-1415(2019).
CC -!- FUNCTION: DNA-binding protein involved in homologous recombination that
CC acts by recruiting the MCM8-MCM9 helicase complex to sites of DNA
CC damage to promote DNA repair synthesis. {ECO:0000269|PubMed:31467087}.
CC -!- SUBUNIT: Interacts with MCM8; this interaction is necessary for MCM8-
CC MCM9 helicase complex recruitment to DNA damage sites
CC (PubMed:31467087). Interacts with RPA1; this interaction associates
CC HROB with the RPA complex (By similarity).
CC {ECO:0000250|UniProtKB:Q32P12, ECO:0000269|PubMed:31467087}.
CC -!- INTERACTION:
CC Q8N3J3-3; Q9UPY8: MAPRE3; NbExp=3; IntAct=EBI-11061081, EBI-726739;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:31467087}. Chromosome
CC {ECO:0000269|PubMed:31467087}. Note=Localized to the sites of DNA
CC damage. {ECO:0000269|PubMed:31467087}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=Q8N3J3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8N3J3-2; Sequence=VSP_025651, VSP_025652;
CC Name=3;
CC IsoId=Q8N3J3-3; Sequence=VSP_025653, VSP_025654, VSP_025655;
CC Name=4;
CC IsoId=Q8N3J3-4; Sequence=VSP_047077;
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DR EMBL; AK291924; BAF84613.1; -; mRNA.
DR EMBL; AK021656; BAB13867.1; -; mRNA.
DR EMBL; AL834295; CAD38968.1; -; mRNA.
DR EMBL; AC004596; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC000121; AAH00121.1; -; mRNA.
DR CCDS; CCDS11477.1; -. [Q8N3J3-1]
DR CCDS; CCDS59293.1; -. [Q8N3J3-4]
DR CCDS; CCDS82136.1; -. [Q8N3J3-3]
DR RefSeq; NP_001164722.1; NM_001171251.2. [Q8N3J3-4]
DR RefSeq; NP_001308239.1; NM_001321310.1. [Q8N3J3-3]
DR RefSeq; NP_001308240.1; NM_001321311.1.
DR RefSeq; NP_076937.2; NM_024032.4. [Q8N3J3-1]
DR AlphaFoldDB; Q8N3J3; -.
DR BioGRID; 122466; 19.
DR IntAct; Q8N3J3; 11.
DR MINT; Q8N3J3; -.
DR STRING; 9606.ENSP00000313500; -.
DR iPTMnet; Q8N3J3; -.
DR PhosphoSitePlus; Q8N3J3; -.
DR BioMuta; C17orf53; -.
DR DMDM; 74714855; -.
DR EPD; Q8N3J3; -.
DR jPOST; Q8N3J3; -.
DR MassIVE; Q8N3J3; -.
DR MaxQB; Q8N3J3; -.
DR PaxDb; Q8N3J3; -.
DR PeptideAtlas; Q8N3J3; -.
DR PRIDE; Q8N3J3; -.
DR ProteomicsDB; 71808; -. [Q8N3J3-1]
DR ProteomicsDB; 71809; -. [Q8N3J3-2]
DR ProteomicsDB; 71810; -. [Q8N3J3-3]
DR Antibodypedia; 17380; 200 antibodies from 19 providers.
DR DNASU; 78995; -.
DR Ensembl; ENST00000245382.6; ENSP00000245382.5; ENSG00000125319.15. [Q8N3J3-3]
DR Ensembl; ENST00000319977.8; ENSP00000313500.4; ENSG00000125319.15. [Q8N3J3-1]
DR Ensembl; ENST00000585683.6; ENSP00000466618.1; ENSG00000125319.15. [Q8N3J3-4]
DR GeneID; 78995; -.
DR KEGG; hsa:78995; -.
DR MANE-Select; ENST00000585683.6; ENSP00000466618.1; NM_001171251.3; NP_001164722.1. [Q8N3J3-4]
DR UCSC; uc002ifi.2; human. [Q8N3J3-1]
DR CTD; 78995; -.
DR DisGeNET; 78995; -.
DR GeneCards; HROB; -.
DR HGNC; HGNC:28460; HROB.
DR HPA; ENSG00000125319; Tissue enhanced (bone marrow, testis).
DR MIM; 618611; gene.
DR neXtProt; NX_Q8N3J3; -.
DR OpenTargets; ENSG00000125319; -.
DR VEuPathDB; HostDB:ENSG00000125319; -.
DR eggNOG; ENOG502QV5E; Eukaryota.
DR GeneTree; ENSGT00400000022305; -.
DR HOGENOM; CLU_028006_0_0_1; -.
DR InParanoid; Q8N3J3; -.
DR OMA; PEDFFCE; -.
DR OrthoDB; 1079551at2759; -.
DR PhylomeDB; Q8N3J3; -.
DR TreeFam; TF331108; -.
DR PathwayCommons; Q8N3J3; -.
DR SignaLink; Q8N3J3; -.
DR BioGRID-ORCS; 78995; 46 hits in 1054 CRISPR screens.
DR ChiTaRS; C17orf53; human.
DR GenomeRNAi; 78995; -.
DR Pharos; Q8N3J3; Tdark.
DR PRO; PR:Q8N3J3; -.
DR Proteomes; UP000005640; Chromosome 17.
DR RNAct; Q8N3J3; protein.
DR Bgee; ENSG00000125319; Expressed in oocyte and 128 other tissues.
DR Genevisible; Q8N3J3; HS.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0090734; C:site of DNA damage; IMP:UniProtKB.
DR GO; GO:0003697; F:single-stranded DNA binding; ISS:UniProtKB.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; IMP:UniProtKB.
DR GO; GO:0000731; P:DNA synthesis involved in DNA repair; IMP:UniProtKB.
DR GO; GO:0007292; P:female gamete generation; ISS:UniProtKB.
DR GO; GO:0036297; P:interstrand cross-link repair; IMP:UniProtKB.
DR GO; GO:0048232; P:male gamete generation; ISS:UniProtKB.
DR GO; GO:0000725; P:recombinational repair; IEA:InterPro.
DR InterPro; IPR028045; HROB.
DR PANTHER; PTHR14523; PTHR14523; 1.
DR Pfam; PF15072; HROB; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Chromosome; DNA damage; DNA recombination;
KW DNA repair; DNA synthesis; DNA-binding; Methylation; Nucleus;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..647
FT /note="Homologous recombination OB-fold protein"
FT /id="PRO_0000288092"
FT REGION 284..361
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 380..399
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 581..631
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 284..309
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 316..337
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 47
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 285
FT /note="Asymmetric dimethylarginine"
FT /evidence="ECO:0007744|PubMed:24129315"
FT MOD_RES 295
FT /note="Asymmetric dimethylarginine"
FT /evidence="ECO:0007744|PubMed:24129315"
FT MOD_RES 329
FT /note="Asymmetric dimethylarginine"
FT /evidence="ECO:0007744|PubMed:24129315"
FT MOD_RES 337
FT /note="Asymmetric dimethylarginine"
FT /evidence="ECO:0007744|PubMed:24129315"
FT VAR_SEQ 108..137
FT /note="SSWIGNQRRVTVTEVLRETARPQSSALHPL -> NCLNSSHSATPLGSLSAT
FT LHCSSTSASPSC (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_025651"
FT VAR_SEQ 138..647
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_025652"
FT VAR_SEQ 409..484
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_025653"
FT VAR_SEQ 484
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_047077"
FT VAR_SEQ 592..597
FT /note="DSGSFQ -> MTWMDS (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_025654"
FT VAR_SEQ 598..647
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_025655"
FT VARIANT 126
FT /note="T -> P (in dbSNP:rs227584)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_032393"
SQ SEQUENCE 647 AA; 69771 MW; 087F0413951BCD43 CRC64;
MACSLQKLFA VEEEFEDEDF LSAVEDAENR FTGSLPVNAG RLRPVSSRPQ ETVQAQSSRL
LLLHPTAPSE ALGLPDLDLC LPASSTPSAD SRPSCIGAAP LRPVSTSSSW IGNQRRVTVT
EVLRETARPQ SSALHPLLTF ESQQQQVGGF EGPEQDEFDK VLASMELEEP GMELECGVSS
EAIPILPAQQ REGSVLAKKA RVVDLSGSCQ KGPVPAIHKA GIMSAQDESL DPVIQCRTPR
PPLRPGAVGH LPVPTALTVP TQQLHWEVCP QRSPVQALQP LQAARGTIQS SPQNRFPCQP
FQSPSSWLSG KAHLPRPRTP NSSCSTPSRT SSGLFPRIPL QPQAPVSSIG SPVGTPKGPQ
GALQTPIVTN HLVQLVTAAS RTPQQPTHPS TRAKTRRFPG PAGILPHQQS GRSLEDIMVS
APQTPTHGAL AKFQTEIVAS SQASVEEDFG RGPWLTMKST LGLDERDPSC FLCTYSIVMV
LRKQAALKQL PRNKVPNMAV MIKSLTRSTM DASVVFKDPT GEMQGTVHRL LLETCQNELK
PGSVLLLKQI GVFSPSLRNH YLNVTPNNLV HIYSPDSGDG SFLKPSQPFP KDSGSFQHDV
AAKPEEGFRT AQNLEAEASP EEELPEADDL DGLLSELPED FFCGTSS
