HROB_MOUSE
ID HROB_MOUSE Reviewed; 615 AA.
AC Q32P12; A2AWS8; Q8CDN3;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 29-MAY-2007, sequence version 2.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=Homologous recombination OB-fold protein {ECO:0000303|PubMed:31467087};
GN Name=Hrob {ECO:0000312|MGI:MGI:2387601};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=129; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP DISRUPTION PHENOTYPE, AND INTERACTION WITH RPA1.
RX PubMed=31467087; DOI=10.1101/gad.329508.119;
RA Hustedt N., Saito Y., Zimmermann M., Alvarez-Quilon A., Setiaputra D.,
RA Adam S., McEwan A., Yuan J.Y., Olivieri M., Zhao Y., Kanemaki M.T.,
RA Jurisicova A., Durocher D.;
RT "Control of homologous recombination by the HROB-MCM8-MCM9 pathway.";
RL Genes Dev. 33:1397-1415(2019).
CC -!- FUNCTION: DNA-binding protein involved in homologous recombination that
CC acts by recruiting the MCM8-MCM9 helicase complex to sites of DNA
CC damage to promote DNA repair synthesis. {ECO:0000250|UniProtKB:Q8N3J3}.
CC -!- SUBUNIT: Interacts with MCM8; this interaction is necessary for MCM8-
CC MCM9 helicase complex recruitment to DNA damage sites (By similarity).
CC Interacts with RPA1; this interaction associates HROB with the RPA
CC complex (PubMed:31467087). {ECO:0000250|UniProtKB:Q8N3J3,
CC ECO:0000269|PubMed:31467087}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q8N3J3}.
CC Chromosome {ECO:0000250|UniProtKB:Q8N3J3}. Note=Localized to the sites
CC of DNA damage. {ECO:0000250|UniProtKB:Q8N3J3}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q32P12-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q32P12-2; Sequence=VSP_025656;
CC -!- DISRUPTION PHENOTYPE: Deficient female and male mice exhibit
CC infertility associated with impaired ovarian development and arrested
CC male meiosis. {ECO:0000269|PubMed:31467087}.
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DR EMBL; AK029811; BAC26629.1; -; mRNA.
DR EMBL; AL954730; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC108338; AAI08339.1; -; mRNA.
DR CCDS; CCDS36342.1; -. [Q32P12-1]
DR RefSeq; NP_705772.3; NM_153544.3. [Q32P12-1]
DR AlphaFoldDB; Q32P12; -.
DR STRING; 10090.ENSMUSP00000097961; -.
DR PhosphoSitePlus; Q32P12; -.
DR EPD; Q32P12; -.
DR MaxQB; Q32P12; -.
DR PaxDb; Q32P12; -.
DR PeptideAtlas; Q32P12; -.
DR PRIDE; Q32P12; -.
DR Antibodypedia; 17380; 200 antibodies from 19 providers.
DR DNASU; 217216; -.
DR Ensembl; ENSMUST00000100392; ENSMUSP00000097961; ENSMUSG00000034773. [Q32P12-1]
DR Ensembl; ENSMUST00000133930; ENSMUSP00000137686; ENSMUSG00000034773. [Q32P12-2]
DR GeneID; 217216; -.
DR KEGG; mmu:217216; -.
DR UCSC; uc007lqz.1; mouse. [Q32P12-1]
DR CTD; 78995; -.
DR MGI; MGI:2387601; Hrob.
DR VEuPathDB; HostDB:ENSMUSG00000034773; -.
DR eggNOG; ENOG502QV5E; Eukaryota.
DR GeneTree; ENSGT00400000022305; -.
DR HOGENOM; CLU_028006_0_0_1; -.
DR InParanoid; Q32P12; -.
DR OMA; PEDFFCE; -.
DR OrthoDB; 1079551at2759; -.
DR PhylomeDB; Q32P12; -.
DR TreeFam; TF331108; -.
DR BioGRID-ORCS; 217216; 6 hits in 74 CRISPR screens.
DR PRO; PR:Q32P12; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q32P12; protein.
DR Bgee; ENSMUSG00000034773; Expressed in animal zygote and 114 other tissues.
DR Genevisible; Q32P12; MM.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0090734; C:site of DNA damage; ISS:UniProtKB.
DR GO; GO:0003697; F:single-stranded DNA binding; IDA:UniProtKB.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; ISO:MGI.
DR GO; GO:0000731; P:DNA synthesis involved in DNA repair; ISS:UniProtKB.
DR GO; GO:0007292; P:female gamete generation; IMP:UniProtKB.
DR GO; GO:0036297; P:interstrand cross-link repair; ISS:UniProtKB.
DR GO; GO:0048232; P:male gamete generation; IMP:UniProtKB.
DR GO; GO:0000725; P:recombinational repair; IEA:InterPro.
DR InterPro; IPR028045; HROB.
DR PANTHER; PTHR14523; PTHR14523; 1.
DR Pfam; PF15072; HROB; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Chromosome; DNA damage; DNA recombination;
KW DNA repair; DNA synthesis; DNA-binding; Methylation; Nucleus;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..615
FT /note="Homologous recombination OB-fold protein"
FT /id="PRO_0000288093"
FT REGION 42..75
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 213..326
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 546..590
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 47..75
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 216..244
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 255..278
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 296..326
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 47
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8N3J3"
FT VAR_SEQ 596..615
FT /note="DDLDGLLSELPEDFFCEPSS -> GGRPAMTPV (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_025656"
FT CONFLICT 118
FT /note="F -> L (in Ref. 3; AAI08339)"
FT /evidence="ECO:0000305"
FT CONFLICT 205
FT /note="E -> G (in Ref. 3; AAI08339)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 615 AA; 66063 MW; 15BFC53F0C637EDE CRC64;
MTCGFQKLFS VEEDFEDEDF LSALENAENH VVSALPRDAG CLRPVSSRPQ ETVQTQSSRP
VPSYPTSNQS VPRLCLPPSS MRENAKAPLS TGVMSLRPAS ISSSSLSSQQ RMTGAKVFQE
SSGPQPSAAH SGYIFESHQQ GIGDFEAPDQ DDLDKALASM EFEGAGLELE ADSGATQILP
AKHCEDPVLA KKARVADLSG SLRKEPMVHC RNPWPSLRPT SATGSLPVPA TSCVSTSQQR
GSPAPAPQYL PVAGRTIRSS PQNYGPGQPL QSPRAWSSGK PRFSGPQRPH GSSAAFCQGP
LSSRAPVSSV ESPVSTPRNT STPVTQPALQ TPVVTNHLVQ LVTATNRTPQ QPSRPSIRAK
TRRFPGPAGL LPHQHSGENL EEIMVSTPQT PTHGALAKFQ TEIATSSQGS VEEDFGQGPW
LTMKSALGLD EGDPTCFLYT YSIVMVLRKA ALKQLPRNKV PNMAVMIKSL TRSTMDASVV
FKDPTGEMLG TVHRVLLETH QSELRPGSVL LLKQIGVFSP SLRNHYLNVT PNNLVHIYSL
DSGDGDFLEP PQPLPKDLGN SHGSLQPDVA AEPTRGLRTA QNPAVAFPEE ELSEADDLDG
LLSELPEDFF CEPSS
