ID   HROB_RAT                Reviewed;         607 AA.
AC   Q6GX86; Q6GX87;
DT   29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=Homologous recombination OB-fold protein;
GN   Name=Hrob {ECO:0000312|RGD:1305385};
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND INDUCTION.
RX   PubMed=16288221; DOI=10.1038/sj.onc.1209210;
RA   Iwanaga R., Komori H., Ishida S., Okamura N., Nakayama K., Nakayama K.,
RA   Ohtani K.;
RT   "Identification of novel E2F1 target genes regulated in cell cycle-
RT   dependent and independent manners.";
RL   Oncogene 25:1786-1798(2006).
CC   -!- FUNCTION: DNA-binding protein involved in homologous recombination that
CC       acts by recruiting the MCM8-MCM9 helicase complex to sites of DNA
CC       damage to promote DNA repair synthesis. {ECO:0000250|UniProtKB:Q8N3J3}.
CC   -!- SUBUNIT: Interacts with MCM8; this interaction is necessary for MCM8-
CC       MCM9 helicase complex recruitment to DNA damage sites (By similarity).
CC       Interacts with RPA1; this interaction associates HROB with the RPA
CC       complex (By similarity). {ECO:0000250|UniProtKB:Q32P12,
CC       ECO:0000250|UniProtKB:Q8N3J3}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q8N3J3}.
CC       Chromosome {ECO:0000250|UniProtKB:Q8N3J3}. Note=Localized to the sites
CC       of DNA damage. {ECO:0000250|UniProtKB:Q8N3J3}.
CC   -!- INDUCTION: By E2F1 and serum stimulation.
CC       {ECO:0000269|PubMed:16288221}.
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DR   EMBL; AY623029; AAT46046.1; -; mRNA.
DR   EMBL; AY623030; AAT46047.1; -; mRNA.
DR   RefSeq; NP_001017988.1; NM_001017988.1.
DR   AlphaFoldDB; Q6GX86; -.
DR   STRING; 10116.ENSRNOP00000063602; -.
DR   PaxDb; Q6GX86; -.
DR   Ensembl; ENSRNOT00000065182; ENSRNOP00000063602; ENSRNOG00000020908.
DR   GeneID; 303566; -.
DR   KEGG; rno:303566; -.
DR   UCSC; RGD:1305385; rat.
DR   CTD; 78995; -.
DR   RGD; 1305385; Hrob.
DR   eggNOG; ENOG502QV5E; Eukaryota.
DR   GeneTree; ENSGT00400000022305; -.
DR   InParanoid; Q6GX86; -.
DR   PhylomeDB; Q6GX86; -.
DR   PRO; PR:Q6GX86; -.
DR   Proteomes; UP000002494; Chromosome 10.
DR   Bgee; ENSRNOG00000020908; Expressed in testis and 14 other tissues.
DR   ExpressionAtlas; Q6GX86; baseline and differential.
DR   Genevisible; Q6GX86; RN.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0090734; C:site of DNA damage; ISS:UniProtKB.
DR   GO; GO:0003697; F:single-stranded DNA binding; ISS:UniProtKB.
DR   GO; GO:0006974; P:cellular response to DNA damage stimulus; ISS:UniProtKB.
DR   GO; GO:0000731; P:DNA synthesis involved in DNA repair; ISS:UniProtKB.
DR   GO; GO:0007292; P:female gamete generation; ISS:UniProtKB.
DR   GO; GO:0036297; P:interstrand cross-link repair; ISS:UniProtKB.
DR   GO; GO:0048232; P:male gamete generation; ISS:UniProtKB.
DR   GO; GO:0000725; P:recombinational repair; IEA:InterPro.
DR   InterPro; IPR028045; HROB.
DR   PANTHER; PTHR14523; PTHR14523; 1.
DR   Pfam; PF15072; HROB; 1.
PE   2: Evidence at transcript level;
KW   Chromosome; DNA damage; DNA recombination; DNA repair; DNA synthesis;
KW   DNA-binding; Methylation; Nucleus; Phosphoprotein; Reference proteome.
FT   CHAIN           1..607
FT                   /note="Homologous recombination OB-fold protein"
FT                   /id="PRO_0000288094"
FT   REGION          25..49
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          84..108
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          196..308
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          531..581
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        28..49
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        208..227
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        235..281
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        288..308
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         30
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q8N3J3"
FT   MOD_RES         281
FT                   /note="Asymmetric dimethylarginine"
FT                   /evidence="ECO:0000250|UniProtKB:Q8N3J3"
SQ   SEQUENCE   607 AA;  65159 MW;  B6BA80E9B9989B3E CRC64;
     MDFLSALENA ENHIVSALPG DAVCLRPNSS RPQETPQAHS SKLSPSYPAS NRSVLGLCFP
     TSRMPETIKE PPCKGAMFLR PVSISSSSSG SQQRMTGTKV SQESSGRQSS AAHSGFIFES
     CQHGIGDFET LDQDEFDKAL ASMEFEGAGL EPEVNRGASQ ILPAKHCEDP VLAKKARVAD
     LSGSFQEGPI VHCRKPWPSL RPTTATGGLP VPATSDISTS HQRGSPVPAP QYSPVAGRTL
     QNGPQNYVPG QPLQSPRAWS SGKPRFSGPQ CPHSSSAAFC RGPSPSRAPV SSVESPFSTP
     RSTSTTVTQP ALQTPVVTNH LVQLVTATNR TPQQPSRPSI RAKTRRFPGP AGLLPHQHSG
     ENLEEIMVST PQTPTHGALA KFQTEIVTSS QGSVEEDFGR GPWLTMKSAL GLDEGDPTCF
     LYTYSIVMVL RKAALKQLPR NKVPNMAVMI KSLTRSTMDA SVVFKDPTGE MLGTVHRVLL
     ETHQNELKPG SVLLLKQIGV FSPSLRNHYL NVTPNNLVHI YSLDSGDGDF LKPPQPLPKD
     LGNSHGSLQP DVAAEPTQGL RTAQNPPASP EEELPEADDL DGLLSELPED FFCEPSGWSC
     LKTGHPP