HRP65_CHITE
ID HRP65_CHITE Reviewed; 535 AA.
AC Q9U1N0; Q95ZG9; Q95ZH0;
DT 19-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 72.
DE RecName: Full=Hrp65 protein;
DE AltName: Full=Ct-Hrp65;
GN Name=HRP65;
OS Chironomus tentans (Midge) (Camptochironomus tentans).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Nematocera; Chironomoidea; Chironomidae;
OC Chironominae; Chironomus.
OX NCBI_TaxID=7153 {ECO:0000312|EMBL:CAB64926.1};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 131-134;
RP 149-154; 216-223; 226-236; 329-341 AND 382-389, AND DEVELOPMENTAL STAGE.
RC TISSUE=Salivary gland;
RX PubMed=10648560; DOI=10.1083/jcb.148.2.271;
RA Miralles F., Oefverstedt L.-G., Sabri N., Aissouni Y., Hellman U.,
RA Skoglund U., Visa N.;
RT "Electron tomography reveals posttranscriptional binding of pre-mRNPs to
RT specific fibers in the nucleoplasm.";
RL J. Cell Biol. 148:271-282(2000).
RN [2] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE (ISOFORMS 1; 2 AND 3), AND SUBCELLULAR LOCATION.
RC TISSUE=Salivary gland;
RX PubMed=11262185; DOI=10.1006/excr.2000.5127;
RA Miralles F., Visa N.;
RT "Molecular characterization of Ct-hrp65: identification of two novel
RT isoforms originated by alternative splicing.";
RL Exp. Cell Res. 264:284-295(2001).
CC -!- FUNCTION: Component of nuclear connecting fibers associated with the
CC transport of ribonucleoprotein particles from either the chromosome to
CC the nuclear pore complex or their transient retention in the
CC nucleoplasm.
CC -!- SUBCELLULAR LOCATION: [Isoform 3]: Cytoplasm, cytoskeleton.
CC -!- SUBCELLULAR LOCATION: [Isoform 2]: Cytoplasm.
CC -!- SUBCELLULAR LOCATION: [Isoform 1]: Nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1 {ECO:0000269|PubMed:11262185};
CC IsoId=Q9U1N0-1; Sequence=Displayed;
CC Name=2 {ECO:0000269|PubMed:11262185};
CC IsoId=Q9U1N0-2; Sequence=VSP_050278;
CC Name=3 {ECO:0000269|PubMed:11262185};
CC IsoId=Q9U1N0-3; Sequence=VSP_050279;
CC -!- DEVELOPMENTAL STAGE: Isoforms 1 and 2 are expressed in embryo, larva
CC and adult while isoform 3 is expressed only in embryo.
CC {ECO:0000269|PubMed:10648560, ECO:0000269|PubMed:11262185}.
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DR EMBL; AJ243013; CAB64926.1; -; mRNA.
DR EMBL; AJ404654; CAC42829.1; -; Genomic_DNA.
DR EMBL; AJ404654; CAC42828.1; -; Genomic_DNA.
DR EMBL; AJ404654; CAC42830.1; -; Genomic_DNA.
DR AlphaFoldDB; Q9U1N0; -.
DR SMR; Q9U1N0; -.
DR PRIDE; Q9U1N0; -.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003723; F:RNA binding; NAS:UniProtKB.
DR GO; GO:0006406; P:mRNA export from nucleus; NAS:UniProtKB.
DR Gene3D; 3.30.70.330; -; 2.
DR InterPro; IPR012975; NOPS.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF08075; NOPS; 1.
DR Pfam; PF00076; RRM_1; 2.
DR SMART; SM00360; RRM; 2.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Cytoskeleton; Direct protein sequencing;
KW Nucleus; Repeat; RNA-binding.
FT CHAIN 1..535
FT /note="Hrp65 protein"
FT /id="PRO_0000081611"
FT DOMAIN 113..185
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 187..268
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 1..80
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 345..411
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 429..535
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 13..63
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 441..506
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 500..535
FT /note="DQGNRFDGPPQRGNVRPWNNNDRGHRDDFQNKRRRY -> YYRPYVNQRPQK
FT ARYRNG (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11262185"
FT /id="VSP_050278"
FT VAR_SEQ 500..535
FT /note="DQGNRFDGPPQRGNVRPWNNNDRGHRDDFQNKRRRY -> KINNYKSTLKNL
FT I (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:11262185"
FT /id="VSP_050279"
SQ SEQUENCE 535 AA; 61891 MW; B6D5BCF701099836 CRC64;
MDVKAEAPNG PAPVKNENQN QPKPQRENMN MNKNQNQNQN NNMGGGGGPN KRNNMNMNKN
FQNRGGKGGP GMGPRGPMKN EDFIVNSKLK NLAGPTHDLP ELVCEEIKFS GRNRLYIGNL
TSDVTEEELK ELFSPYGEIS EAFINAEKNF AFLKIDYRAN AERAKKDLDG RMRKNKPIRI
RFAPNATTIR VKNLTPFVSN ELLFKSFEVF GQVERAVIIV DDRGKTTGEG IVEFARKSGA
MSALKYCSEK CYFLTSSLRP CVVETFDHID ETDGFPEKSL MRKSNDYYKA RQNGPRFAEM
GSFEHEFGTK WKQMYDMYKQ KHDALKREMQ LEEEKLEAQM EYAKFEHETE SLREQLRKRE
QDRDRQKKEW EDRERQADES RIRDEQQMRR QQDDMQMRMQ RQDEEMRRRQ QENSLFMQAQ
QLSNMLDQQE MNHQGGGGGG GNGGNGNNQG GGGNQGGGRR NYNNDRNNDR NQNFDMMNQG
GGNHGGNQYQ GNQHYQGNQD QGNRFDGPPQ RGNVRPWNNN DRGHRDDFQN KRRRY
