HRPA_ECOLI
ID HRPA_ECOLI Reviewed; 1300 AA.
AC P43329; P76861; P76863; P77479;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 27-APR-2001, sequence version 3.
DT 03-AUG-2022, entry version 168.
DE RecName: Full=ATP-dependent RNA helicase HrpA;
DE EC=3.6.4.13;
GN Name=hrpA; OrderedLocusNames=b1413, JW5905;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=7899078; DOI=10.1093/nar/23.4.595;
RA Moriya H., Kasai H., Isono K.;
RT "Cloning and characterization of the hrpA gene in the terC region of
RT Escherichia coli that is highly similar to the DEAH family RNA helicase
RT genes of Saccharomyces cerevisiae.";
RL Nucleic Acids Res. 23:595-598(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K.,
RA Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S.,
RA Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G.,
RA Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y.,
RA Wada C., Yamamoto Y., Horiuchi T.;
RT "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 28.0-40.1 min region on the linkage map.";
RL DNA Res. 3:363-377(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
CC -!- FUNCTION: Not yet known.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DEAH subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA07685.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAA15029.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; D42105; BAA07685.1; ALT_INIT; Genomic_DNA.
DR EMBL; U00096; AAC74495.2; -; Genomic_DNA.
DR EMBL; AP009048; BAA15029.1; ALT_INIT; Genomic_DNA.
DR PIR; H64892; H64892.
DR RefSeq; NP_415931.4; NC_000913.3.
DR RefSeq; WP_000139543.1; NZ_SSZK01000021.1.
DR PDB; 6ZWW; X-ray; 3.16 A; A/C/E/G=1-783.
DR PDB; 6ZWX; X-ray; 2.70 A; A=1-756.
DR PDB; 7AKP; X-ray; 2.59 A; A=1-783.
DR PDBsum; 6ZWW; -.
DR PDBsum; 6ZWX; -.
DR PDBsum; 7AKP; -.
DR AlphaFoldDB; P43329; -.
DR SMR; P43329; -.
DR BioGRID; 4261507; 128.
DR DIP; DIP-9937N; -.
DR IntAct; P43329; 26.
DR STRING; 511145.b1413; -.
DR jPOST; P43329; -.
DR PaxDb; P43329; -.
DR PRIDE; P43329; -.
DR EnsemblBacteria; AAC74495; AAC74495; b1413.
DR EnsemblBacteria; BAA15029; BAA15029; BAA15029.
DR GeneID; 948444; -.
DR KEGG; ecj:JW5905; -.
DR KEGG; eco:b1413; -.
DR PATRIC; fig|1411691.4.peg.858; -.
DR EchoBASE; EB2772; -.
DR eggNOG; COG1643; Bacteria.
DR HOGENOM; CLU_001832_3_3_6; -.
DR InParanoid; P43329; -.
DR OMA; YERVTLY; -.
DR PhylomeDB; P43329; -.
DR BioCyc; EcoCyc:G6732-MON; -.
DR BioCyc; MetaCyc:G6732-MON; -.
DR PRO; PR:P43329; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0004386; F:helicase activity; IMP:EcoliWiki.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0003724; F:RNA helicase activity; ISS:EcoCyc.
DR GO; GO:0000462; P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IBA:GO_Central.
DR GO; GO:0006397; P:mRNA processing; IMP:EcoCyc.
DR GO; GO:0009451; P:RNA modification; IMP:EcoliWiki.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR011709; DEAD-box_helicase_OB_fold.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR007502; Helicase-assoc_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR024590; HrpA_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR010222; RNA_helicase_HrpA.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF11898; DUF3418; 1.
DR Pfam; PF04408; HA2; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF07717; OB_NTP_bind; 1.
DR SMART; SM00382; AAA; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00847; HA2; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR01967; DEAH_box_HrpA; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Helicase; Hydrolase; Nucleotide-binding;
KW Reference proteome.
FT CHAIN 1..1300
FT /note="ATP-dependent RNA helicase HrpA"
FT /id="PRO_0000055178"
FT DOMAIN 87..250
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 274..444
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT MOTIF 197..200
FT /note="DEAH box"
FT BINDING 100..107
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT CONFLICT 152
FT /note="G -> V (in Ref. 1; BAA07685)"
FT /evidence="ECO:0000305"
FT CONFLICT 202
FT /note="R -> H (in Ref. 1; BAA07685)"
FT /evidence="ECO:0000305"
FT CONFLICT 301
FT /note="Missing (in Ref. 1; BAA07685)"
FT /evidence="ECO:0000305"
FT CONFLICT 304
FT /note="R -> P (in Ref. 1; BAA07685)"
FT /evidence="ECO:0000305"
FT CONFLICT 681..694
FT /note="GRIAARIDPEWVEP -> AHCCVSTRNGGA (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 705
FT /note="S -> T (in Ref. 1; BAA07685)"
FT /evidence="ECO:0000305"
FT CONFLICT 712..713
FT /note="AQ -> E (in Ref. 1; BAA07685)"
FT /evidence="ECO:0000305"
FT CONFLICT 869
FT /note="A -> P (in Ref. 1; BAA07685)"
FT /evidence="ECO:0000305"
FT HELIX 9..14
FT /evidence="ECO:0007829|PDB:7AKP"
FT HELIX 15..17
FT /evidence="ECO:0007829|PDB:7AKP"
FT HELIX 21..32
FT /evidence="ECO:0007829|PDB:7AKP"
FT TURN 33..36
FT /evidence="ECO:0007829|PDB:7AKP"
FT HELIX 40..66
FT /evidence="ECO:0007829|PDB:7AKP"
FT TURN 79..82
FT /evidence="ECO:0007829|PDB:7AKP"
FT HELIX 83..91
FT /evidence="ECO:0007829|PDB:7AKP"
FT STRAND 94..100
FT /evidence="ECO:0007829|PDB:7AKP"
FT HELIX 105..117
FT /evidence="ECO:0007829|PDB:7AKP"
FT TURN 118..122
FT /evidence="ECO:0007829|PDB:7AKP"
FT STRAND 123..131
FT /evidence="ECO:0007829|PDB:7AKP"
FT HELIX 132..145
FT /evidence="ECO:0007829|PDB:7AKP"
FT STRAND 152..161
FT /evidence="ECO:0007829|PDB:7AKP"
FT STRAND 170..174
FT /evidence="ECO:0007829|PDB:7AKP"
FT HELIX 175..180
FT /evidence="ECO:0007829|PDB:7AKP"
FT TURN 181..184
FT /evidence="ECO:0007829|PDB:7AKP"
FT STRAND 191..196
FT /evidence="ECO:0007829|PDB:7AKP"
FT HELIX 199..201
FT /evidence="ECO:0007829|PDB:7AKP"
FT HELIX 204..219
FT /evidence="ECO:0007829|PDB:7AKP"
FT STRAND 224..229
FT /evidence="ECO:0007829|PDB:7AKP"
FT STRAND 231..233
FT /evidence="ECO:0007829|PDB:6ZWW"
FT HELIX 235..240
FT /evidence="ECO:0007829|PDB:7AKP"
FT STRAND 241..243
FT /evidence="ECO:0007829|PDB:6ZWX"
FT STRAND 246..249
FT /evidence="ECO:0007829|PDB:7AKP"
FT STRAND 257..260
FT /evidence="ECO:0007829|PDB:7AKP"
FT HELIX 276..288
FT /evidence="ECO:0007829|PDB:7AKP"
FT STRAND 293..296
FT /evidence="ECO:0007829|PDB:7AKP"
FT STRAND 298..300
FT /evidence="ECO:0007829|PDB:7AKP"
FT HELIX 301..313
FT /evidence="ECO:0007829|PDB:7AKP"
FT STRAND 320..322
FT /evidence="ECO:0007829|PDB:7AKP"
FT STRAND 325..327
FT /evidence="ECO:0007829|PDB:6ZWX"
FT HELIX 329..332
FT /evidence="ECO:0007829|PDB:7AKP"
FT TURN 333..336
FT /evidence="ECO:0007829|PDB:7AKP"
FT STRAND 344..347
FT /evidence="ECO:0007829|PDB:7AKP"
FT HELIX 349..352
FT /evidence="ECO:0007829|PDB:6ZWX"
FT STRAND 362..365
FT /evidence="ECO:0007829|PDB:7AKP"
FT STRAND 368..370
FT /evidence="ECO:0007829|PDB:7AKP"
FT STRAND 373..375
FT /evidence="ECO:0007829|PDB:7AKP"
FT TURN 376..379
FT /evidence="ECO:0007829|PDB:7AKP"
FT STRAND 380..382
FT /evidence="ECO:0007829|PDB:7AKP"
FT STRAND 385..387
FT /evidence="ECO:0007829|PDB:7AKP"
FT HELIX 390..397
FT /evidence="ECO:0007829|PDB:7AKP"
FT HELIX 398..400
FT /evidence="ECO:0007829|PDB:6ZWX"
FT STRAND 401..405
FT /evidence="ECO:0007829|PDB:7AKP"
FT STRAND 407..412
FT /evidence="ECO:0007829|PDB:7AKP"
FT HELIX 414..418
FT /evidence="ECO:0007829|PDB:7AKP"
FT HELIX 427..429
FT /evidence="ECO:0007829|PDB:7AKP"
FT HELIX 433..442
FT /evidence="ECO:0007829|PDB:7AKP"
FT HELIX 448..450
FT /evidence="ECO:0007829|PDB:7AKP"
FT HELIX 460..471
FT /evidence="ECO:0007829|PDB:7AKP"
FT STRAND 475..479
FT /evidence="ECO:0007829|PDB:6ZWW"
FT STRAND 484..486
FT /evidence="ECO:0007829|PDB:6ZWW"
FT HELIX 488..495
FT /evidence="ECO:0007829|PDB:7AKP"
FT STRAND 496..498
FT /evidence="ECO:0007829|PDB:6ZWX"
FT HELIX 500..509
FT /evidence="ECO:0007829|PDB:7AKP"
FT TURN 510..513
FT /evidence="ECO:0007829|PDB:7AKP"
FT HELIX 515..525
FT /evidence="ECO:0007829|PDB:7AKP"
FT HELIX 535..537
FT /evidence="ECO:0007829|PDB:6ZWX"
FT HELIX 539..545
FT /evidence="ECO:0007829|PDB:7AKP"
FT HELIX 546..548
FT /evidence="ECO:0007829|PDB:7AKP"
FT STRAND 551..555
FT /evidence="ECO:0007829|PDB:7AKP"
FT HELIX 556..569
FT /evidence="ECO:0007829|PDB:7AKP"
FT HELIX 574..583
FT /evidence="ECO:0007829|PDB:7AKP"
FT HELIX 588..607
FT /evidence="ECO:0007829|PDB:7AKP"
FT HELIX 619..627
FT /evidence="ECO:0007829|PDB:7AKP"
FT HELIX 631..633
FT /evidence="ECO:0007829|PDB:6ZWX"
FT STRAND 634..637
FT /evidence="ECO:0007829|PDB:7AKP"
FT STRAND 644..646
FT /evidence="ECO:0007829|PDB:6ZWX"
FT HELIX 647..649
FT /evidence="ECO:0007829|PDB:7AKP"
FT STRAND 651..654
FT /evidence="ECO:0007829|PDB:7AKP"
FT STRAND 661..663
FT /evidence="ECO:0007829|PDB:7AKP"
FT STRAND 666..686
FT /evidence="ECO:0007829|PDB:7AKP"
FT HELIX 689..691
FT /evidence="ECO:0007829|PDB:7AKP"
FT HELIX 693..695
FT /evidence="ECO:0007829|PDB:7AKP"
FT TURN 697..699
FT /evidence="ECO:0007829|PDB:7AKP"
FT STRAND 701..710
FT /evidence="ECO:0007829|PDB:7AKP"
FT TURN 711..714
FT /evidence="ECO:0007829|PDB:7AKP"
FT STRAND 715..724
FT /evidence="ECO:0007829|PDB:7AKP"
FT STRAND 727..734
FT /evidence="ECO:0007829|PDB:7AKP"
FT HELIX 737..740
FT /evidence="ECO:0007829|PDB:7AKP"
FT HELIX 743..749
FT /evidence="ECO:0007829|PDB:7AKP"
SQ SEQUENCE 1300 AA; 149028 MW; A26601266D771638 CRC64;
MTEQQKLTFT ALQQRLDSLM LRDRLRFSRR LHGVKKVKNP DAQQAIFQEM AKEIDQAAGK
VLLREAARPE ITYPDNLPVS QKKQDILEAI RDHQVVIVAG ETGSGKTTQL PKICMELGRG
IKGLIGHTQP RRLAARTVAN RIAEELKTEP GGCIGYKVRF SDHVSDNTMV KLMTDGILLA
EIQQDRLLMQ YDTIIIDEAH ERSLNIDFLL GYLKELLPRR PDLKIIITSA TIDPERFSRH
FNNAPIIEVS GRTYPVEVRY RPIVEEADDT ERDQLQAIFD AVDELSQESH GDILIFMSGE
REIRDTADAL NKLNLRHTEI LPLYARLSNS EQNRVFQSHS GRRIVLATNV AETSLTVPGI
KYVIDPGTAR ISRYSYRTKV QRLPIEPISQ ASANQRKGRC GRVSEGICIR LYSEDDFLSR
PEFTDPEILR TNLASVILQM TALGLGDIAA FPFVEAPDKR NIQDGVRLLE ELGAITTDEQ
ASAYKLTPLG RQLSQLPVDP RLARMVLEAQ KHGCVREAMI ITSALSIQDP RERPMDKQQA
SDEKHRRFHD KESDFLAFVN LWNYLGEQQK ALSSNAFRRL CRTDYLNYLR VREWQDIYTQ
LRQVVKELGI PVNSEPAEYR EIHIALLTGL LSHIGMKDAD KQEYTGARNA RFSIFPGSGL
FKKPPKWVMV AELVETSRLW GRIAARIDPE WVEPVAQHLI KRTYSEPHWE RAQGAVMATE
KVTVYGLPIV AARKVNYSQI DPALCRELFI RHALVEGDWQ TRHAFFRENL KLRAEVEELE
HKSRRRDILV DDETLFEFYD QRISHDVISA RHFDSWWKKV SRETPDLLNF EKSMLIKEGA
EKISKLDYPN FWHQGNLKLR LSYQFEPGAD ADGVTVHIPL PLLNQVEESG FEWQIPGLRR
ELVIALIKSL PKPVRRNFVP APNYAEAFLG RVKPLELPLL DSLERELRRM TGVTVDREDW
HWDQVPDHLK ITFRVVDDKN KKLKEGRSLQ DLKDALKGKV QETLSAVADD GIEQSGLHIW
SFGQLPESYE QKRGNYKVKA WPALVDERDS VAIKLFDNPL EQKQAMWNGL RRLLLLNIPS
PIKYLHEKLP NKAKLGLYFN PYGKVLELID DCISCGVDKL IDANGGPVWT EEGFAALHEK
VRAELNDTVV DIAKQVEQIL TAVFNINKRL KGRVDMTMAL GLSDIKAQMG GLVYRGFVTG
NGFKRLGDTL RYLQAIEKRL EKLAVDPHRD RAQMLKVENV QQAWQQWINK LPPARREDED
VKEIRWMIEE LRVSYFAQQL GTPYPISDKR ILQAMEQISG
