HRPA_PSESM
ID HRPA_PSESM Reviewed; 113 AA.
AC Q52473; Q7C4I1;
DT 07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 25-MAY-2022, entry version 104.
DE RecName: Full=Hrp pili protein HrpA;
DE AltName: Full=TTSS pilin HrpA;
GN Name=hrpA; Synonyms=hrpA1; OrderedLocusNames=PSPTO_1381;
OS Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=223283;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC BAA-871 / DC3000;
RX PubMed=7579616; DOI=10.1094/mpmi-8-0717;
RA Preston G., Huang H.-C., He S.Y., Collmer A.;
RT "The HrpZ proteins of Pseudomonas syringae pvs. syringae, glycinea, and
RT tomato are encoded by an operon containing Yersinia ysc homologs and elicit
RT the hypersensitive response in tomato but not soybean.";
RL Mol. Plant Microbe Interact. 8:717-732(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC BAA-871 / DC3000;
RX PubMed=12580281; DOI=10.1094/mpmi.2003.16.1.43;
RA Fouts D.E., Badel J.L., Ramos A.R., Rapp R.A., Collmer A.;
RT "A Pseudomonas syringae pv. tomato DC3000 Hrp (type III secretion) deletion
RT mutant expressing the Hrp system of bean pathogen P. syringae pv. syringae
RT 61 retains normal host specificity for tomato.";
RL Mol. Plant Microbe Interact. 16:43-52(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ICMP 2844;
RA Inoue Y., Takikawa Y.;
RT "Phylogenic analysis of DNA sequences around the hrpZ regions of
RT Pseudomonas syringae.";
RL Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-871 / DC3000;
RX PubMed=12928499; DOI=10.1073/pnas.1731982100;
RA Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T.,
RA Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C.,
RA Davidsen T.M., Zafar N., Zhou L., Liu J., Yuan Q., Khouri H.M.,
RA Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R.,
RA Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R.,
RA Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G.,
RA Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M.,
RA Collmer A.;
RT "The complete genome sequence of the Arabidopsis and tomato pathogen
RT Pseudomonas syringae pv. tomato DC3000.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003).
RN [5]
RP PROTEIN SEQUENCE OF 2-11; 16-25 AND 35-50, MASS SPECTROMETRY, AND FUNCTION.
RC STRAIN=ATCC BAA-871 / DC3000;
RX PubMed=9395289; DOI=10.1016/s0014-5793(97)01276-3;
RA Roine E., Saarinen J., Kalkkinen N., Romantschuk M.;
RT "Purified HrpA of Pseudomonas syringae pv. tomato DC3000 reassembles into
RT pili.";
RL FEBS Lett. 417:168-172(1997).
RN [6]
RP PROTEIN SEQUENCE OF 2-36, AND FUNCTION.
RC STRAIN=ATCC BAA-871 / DC3000;
RX PubMed=9096416; DOI=10.1073/pnas.94.7.3459;
RA Roine E., Wei W., Yuan J., Nurmiaho-Lassila E.-L., Kalkkinen N.,
RA Romantschuk M., He S.Y.;
RT "Hrp pilus: an hrp-dependent bacterial surface appendage produced by
RT Pseudomonas syringae pv. tomato DC3000.";
RL Proc. Natl. Acad. Sci. U.S.A. 94:3459-3464(1997).
RN [7]
RP MUTAGENESIS.
RC STRAIN=ATCC BAA-871 / DC3000;
RX PubMed=10564513; DOI=10.1046/j.1365-2958.1999.01635.x;
RA Taira S., Tuimala J., Roine E., Nurmiaho-Lassila E.-L., Savilahti H.,
RA Romantschuk M.;
RT "Mutational analysis of the Pseudomonas syringae pv. tomato hrpA gene
RT encoding Hrp pilus subunit.";
RL Mol. Microbiol. 34:737-744(1999).
RN [8]
RP FUNCTION, REGULATION OF HRP-ASSOCIATED GENES, AND MUTAGENESIS OF GLY-23;
RP ALA-54; LYS-93; GLU-94; ASP-95; ILE-101 AND ILE-111.
RC STRAIN=ATCC BAA-871 / DC3000;
RX PubMed=10681465; DOI=10.1073/pnas.040570097;
RA Wei W., Plovanich-Jones A., Deng W.-L., Jin Q.-L., Collmer A., Huang H.-C.,
RA He S.Y.;
RT "The gene coding for the Hrp pilus structural protein is required for type
RT III secretion of Hrp and Avr proteins in Pseudomonas syringae pv. tomato.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:2247-2252(2000).
RN [9]
RP FUNCTION OF HRP PILUS.
RC STRAIN=ATCC BAA-871 / DC3000;
RX PubMed=11401717; DOI=10.1046/j.1365-2958.2001.02455.x;
RA Jin Q.-L., Hu W., Brown I., McGhee G., Hart P., Jones A.L., He S.Y.;
RT "Visualization of secreted Hrp and Avr proteins along the Hrp pilus during
RT type III secretion in Erwinia amylovora and Pseudomonas syringae.";
RL Mol. Microbiol. 40:1129-1139(2001).
RN [10]
RP FUNCTION OF HRP PILUS.
RC STRAIN=ATCC BAA-871 / DC3000;
RX PubMed=11752577; DOI=10.1126/science.1066397;
RA Jin Q.-L., He S.Y.;
RT "Role of the Hrp pilus in type III protein secretion in Pseudomonas
RT syringae.";
RL Science 294:2556-2558(2001).
RN [11]
RP SECRETION SIGNAL.
RC STRAIN=ATCC BAA-871 / DC3000;
RX PubMed=11862491; DOI=10.1007/s00438-001-0619-4;
RA Hienonen E., Roine E., Romantschuk M., Taira S.;
RT "mRNA stability and the secretion signal of HrpA, a pilin secreted by the
RT type III system in Pseudomonas syringae.";
RL Mol. Genet. Genomics 266:973-978(2002).
CC -!- FUNCTION: Major structural protein of the Hrp pilus, which is a
CC component of the type III secretion system (TTSS, Hrp secretion system)
CC required for effector protein delivery, parasitism, and pathogenicity.
CC Required for secretion of virulence proteins such as HrpW and AvrPto.
CC The Hrp pilus functions as a conduit for protein delivery into the host
CC cell. Also, affects the expression of TTSS-associated genes. Required
CC for full expression of genes that encode regulatory, secretion, and
CC effector proteins of the TTSS. HrpA-mediated gene regulation apparently
CC is through effect on the mRNA level of hrpR and hrpS.
CC {ECO:0000269|PubMed:10681465, ECO:0000269|PubMed:11401717,
CC ECO:0000269|PubMed:11752577, ECO:0000269|PubMed:9096416,
CC ECO:0000269|PubMed:9395289}.
CC -!- SUBCELLULAR LOCATION: Secreted. Fimbrium. Note=Extracellular, secreted
CC via type III secretion system.
CC -!- INDUCTION: Expression enhanced by contact with host cell wall. Also
CC regulated by HrpRS and HrpL.
CC -!- PTM: HrpA might be cleaved/degraded in 3 forms but it is not known if
CC the observed processing has any biological significance.
CC -!- MASS SPECTROMETRY: Mass=11270; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:9395289};
CC -!- MISCELLANEOUS: Analysis of mutants revealed that the C-terminus is
CC important for pilus assembly and secretion and / or regulation function
CC of HprA.
CC -!- SIMILARITY: Belongs to the HrpA type 1 family. {ECO:0000305}.
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DR EMBL; AF232004; AAB00126.1; -; Genomic_DNA.
DR EMBL; AB112567; BAD20879.1; -; Genomic_DNA.
DR EMBL; AE016853; AAO54903.1; -; Genomic_DNA.
DR RefSeq; NP_791208.1; NC_004578.1.
DR RefSeq; WP_005763904.1; NC_004578.1.
DR AlphaFoldDB; Q52473; -.
DR SMR; Q52473; -.
DR STRING; 223283.PSPTO_1381; -.
DR EnsemblBacteria; AAO54903; AAO54903; PSPTO_1381.
DR GeneID; 1183017; -.
DR KEGG; pst:PSPTO_1381; -.
DR PATRIC; fig|223283.9.peg.1403; -.
DR eggNOG; ENOG5032GQ2; Bacteria.
DR HOGENOM; CLU_2131313_0_0_6; -.
DR OMA; SSKAMED; -.
DR OrthoDB; 1977653at2; -.
DR Proteomes; UP000002515; Chromosome.
DR GO; GO:0005615; C:extracellular space; IEA:InterPro.
DR GO; GO:0009289; C:pilus; IEA:UniProtKB-SubCell.
DR InterPro; IPR018581; T3SS_pilus_HrpA.
DR Pfam; PF09589; HrpA_pilin; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Fimbrium; Reference proteome; Secreted;
KW Virulence.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:9096416,
FT ECO:0000269|PubMed:9395289"
FT CHAIN 2..113
FT /note="Hrp pili protein HrpA"
FT /id="PRO_0000219574"
FT MUTAGEN 23
FT /note="G->A: No effect."
FT /evidence="ECO:0000269|PubMed:10681465"
FT MUTAGEN 54
FT /note="A->E: No effect."
FT /evidence="ECO:0000269|PubMed:10681465"
FT MUTAGEN 93
FT /note="K->I: No effect."
FT /evidence="ECO:0000269|PubMed:10681465"
FT MUTAGEN 94
FT /note="E->K: Affects the regulatory function."
FT /evidence="ECO:0000269|PubMed:10681465"
FT MUTAGEN 95
FT /note="D->S: Affects the secretion function."
FT /evidence="ECO:0000269|PubMed:10681465"
FT MUTAGEN 101
FT /note="I->T: No effect."
FT /evidence="ECO:0000269|PubMed:10681465"
FT MUTAGEN 111
FT /note="I->P: Affects the secretion function."
FT /evidence="ECO:0000269|PubMed:10681465"
SQ SEQUENCE 113 AA; 11387 MW; 8E6C28F26B8FB080 CRC64;
MVAFAGLTSK LTNLGNSAVG GVGGALQGVN TVASNATLQK NILLGTGDSL SVDAQAKASK
ESDANGAKLI AMQAQETMKK QTMDVLNAIQ AGKEDSTNKK ISATATNAKG ISY
