ID   HRPI_ERWAM              Reviewed;         715 AA.
AC   P35654;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 2.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Harpin secretion protein HrpI;
GN   Name=hrpI;
OS   Erwinia amylovora (Fire blight bacteria).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Erwinia.
OX   NCBI_TaxID=552;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Ea321;
RX   PubMed=8253684; DOI=10.1128/jb.175.24.7958-7967.1993;
RA   Wei Z.-M., Beer S.V.;
RT   "HrpI of Erwinia amylovora functions in secretion of harpin and is a member
RT   of a new protein family.";
RL   J. Bacteriol. 175:7958-7967(1993).
RN   [2]
RP   SEQUENCE REVISION.
RA   Beer S.V.;
RL   Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in the secretion of harpin; a proteinaceous elicitor
CC       of the hypersensitivity response in plants.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC       membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the FHIPEP (flagella/HR/invasion proteins export
CC       pore) family. {ECO:0000305}.
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DR   EMBL; L25828; AAB05999.2; -; Genomic_DNA.
DR   PIR; A49913; A49913.
DR   RefSeq; WP_004155349.1; NZ_RQKG01000002.1.
DR   AlphaFoldDB; P35654; -.
DR   SMR; P35654; -.
DR   GeneID; 8913115; -.
DR   OMA; ECGHLPP; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0034053; P:modulation by symbiont of host defense-related programmed cell death; IEA:UniProtKB-KW.
DR   GO; GO:0009306; P:protein secretion; IEA:InterPro.
DR   Gene3D; 1.10.8.540; -; 1.
DR   Gene3D; 3.40.30.60; -; 1.
DR   Gene3D; 3.40.50.12790; -; 1.
DR   InterPro; IPR042194; FHIPEP_1.
DR   InterPro; IPR042193; FHIPEP_3.
DR   InterPro; IPR042196; FHIPEP_4.
DR   InterPro; IPR025505; FHIPEP_CS.
DR   InterPro; IPR001712; T3SS_FHIPEP.
DR   InterPro; IPR006302; T3SS_HrcV.
DR   PANTHER; PTHR30161; PTHR30161; 1.
DR   Pfam; PF00771; FHIPEP; 1.
DR   PIRSF; PIRSF005419; FlhA; 1.
DR   PRINTS; PR00949; TYPE3IMAPROT.
DR   TIGRFAMs; TIGR01399; hrcV; 1.
DR   PROSITE; PS00994; FHIPEP; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Hypersensitive response elicitation;
KW   Membrane; Protein transport; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..715
FT                   /note="Harpin secretion protein HrpI"
FT                   /id="PRO_0000190024"
FT   TRANSMEM        23..43
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        45..65
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        69..89
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        115..135
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        203..223
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        241..261
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        298..318
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   715 AA;  79505 MW;  D42373616446401B CRC64;
     MSSLFVWLNR LAISAMQRSE VVGAAIVMSI VFMMIIPLPT GLIDVLIALN ICISSLLIVL
     AMYLPKPLAF STFPSVLLLT TMFRLALSIS TTRQILLQQD AGHIVEAFGN FVVGGNLAVG
     LVIFLILTVV NFLVITKGSE RVAEVAARFT LDAMPGKQMS IDSDLRAGLI EAHQARQRRE
     NLAKESQLFG AMDGAMKFVK GDAIAGLVIV FINMIGGFAI GVLQNGMEAG AAMHIYSVLT
     IGDGLIAQIP ALLISLTAGM IITRVSADGQ QVDANIGREI AEQLTSQPKA WIMSAAGMLG
     FALLPGMPTA VFVIISAIAL GSGLFQLWRI KQQDSQQQAD ELHAQQLAPE DNGYQDLRRF
     NPTRAYLLQF SQEHLNSEAA ESLIQHIRRL RNRLVYHFGF TLPSFDIEFS PALAADEFRF
     CVYEIPLVTA TFAVEQLAVR TSSIEIHLAD GESDGQIQPG QAERDEHHWC WLPPQHPLLQ
     QEDRRCWNAQ QLIMLRMEQA IHQSGAQFIG LQESKSILNW LESEQPELAQ ELQRIMPLSR
     FAAVLQRLAS ERIPLRSVRT IAETLIEHGQ HERDSAALTD FVRIALKEHI CHQYQQPNGL
     NVWLLTPETE ELLRDSLRQA QSETFFSLAQ EYGINLLNQM RSAFPPYDNH HALILVAQDL
     RSPLRALLKD EFHAVPVLSF AELTSNVAIN VLGRLDLQQS PPELQENDPC MNYAY