ID   HRPS_PSESY              Reviewed;         302 AA.
AC   P37931;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=Pathogenicity locus probable regulatory protein HrpS;
GN   Name=hrpS;
OS   Pseudomonas syringae pv. syringae.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas; Pseudomonas syringae.
OX   NCBI_TaxID=321;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Pss61;
RX   PubMed=8106313; DOI=10.1128/jb.176.4.1025-1036.1994;
RA   Xiao Y., Heu S., Yi J., Lu Y., Hutcheson S.W.;
RT   "Identification of a putative alternate sigma factor and characterization
RT   of a multicomponent regulatory cascade controlling the expression of
RT   Pseudomonas syringae pv. syringae Pss61 hrp and hrmA genes.";
RL   J. Bacteriol. 176:1025-1036(1994).
CC   -!- FUNCTION: Regulates the activation of the sigma factor HrpL which
CC       itself induces the expression of hprD as well as other hrp loci which
CC       are involved in plant pathogenicity, hrmA and avr genes. Probably
CC       interacts with sigma-54.
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DR   EMBL; U03852; AAA17650.1; -; Unassigned_DNA.
DR   PIR; B49889; B49889.
DR   RefSeq; WP_003365153.1; NZ_MLEV01000001.1.
DR   AlphaFoldDB; P37931; -.
DR   SMR; P37931; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0034053; P:modulation by symbiont of host defense-related programmed cell death; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR002197; HTH_Fis.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002078; Sigma_54_int.
DR   InterPro; IPR025943; Sigma_54_int_dom_ATP-bd_2.
DR   InterPro; IPR025944; Sigma_54_int_dom_CS.
DR   Pfam; PF02954; HTH_8; 1.
DR   Pfam; PF00158; Sigma54_activat; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00676; SIGMA54_INTERACT_2; 1.
DR   PROSITE; PS00688; SIGMA54_INTERACT_3; 1.
DR   PROSITE; PS50045; SIGMA54_INTERACT_4; 1.
PE   4: Predicted;
KW   Activator; ATP-binding; DNA-binding; Hypersensitive response elicitation;
KW   Nucleotide-binding; Transcription; Transcription regulation;
KW   Two-component regulatory system.
FT   CHAIN           1..302
FT                   /note="Pathogenicity locus probable regulatory protein
FT                   HrpS"
FT                   /id="PRO_0000081323"
FT   DOMAIN          9..237
FT                   /note="Sigma-54 factor interaction"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   DNA_BIND        279..298
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255"
FT   BINDING         37..44
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   BINDING         99..108
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
SQ   SEQUENCE   302 AA;  33410 MW;  0F1955FC0AD52CDF CRC64;
     MNLDDEFDDD LDAERVPNLG IVAESISQLG IDVLLSGETG TGKDTTARRI HNMSGRQGRF
     VPMNCAAIPE SLAESELFGV VSGAYTGADR SRMGYIEAAQ GGTLYLDEID SMPLALQAKL
     LRVLETRALE RLGSTSTIHL DICVIASAQA SLDDAVEEGK FRRDLYFRLN VLTLKLPPLR
     DQPERILPLF TRFVAASAKE LNVAIPDVCP LLQQVLTGHR WPGNIRELKA AAKRHVLGFP
     LLGADSQTEE HMACGLKFQL RAIEKALIQQ ALKRHRNCID AASLELDIPR RTLYRRIKEL
     SI