ID   HRPZ_PSEAP              Reviewed;         342 AA.
AC   O87653;
DT   26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   25-MAY-2022, entry version 52.
DE   RecName: Full=Harpin HrpZ;
DE   AltName: Full=Harpin-Psa;
DE   AltName: Full=HrpZ-Psa protein;
GN   Name=hrpZ;
OS   Pseudomonas syringae pv. aptata.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas; Pseudomonas syringae.
OX   NCBI_TaxID=83167;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-10.
RC   STRAIN=LMG 5532 / NCPPB 2664;
RX   PubMed=11392485; DOI=10.1023/a:1010280116487;
RA   Musa A.R., Minard P., Mazzucchi U.;
RT   "Identification and expression of the Pseudomonas syringae pv. aptata
RT   hrpZ(Psa) gene which encodes an harpin elicitor.";
RL   Antonie Van Leeuwenhoek 79:61-71(2001).
CC   -!- FUNCTION: Harpins are proteins able to elicit hypersensitive response
CC       (HR) in non-host plants and are required for pathogenicity in host
CC       plants. HrpZ forms ion-conducting pores permeable for cations. Such
CC       pore-forming activity may allow nutrient release and/or delivery of
CC       virulence factors during bacterial colonization of host plants (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homomultimeric. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted. Host cell membrane {ECO:0000305}.
CC       Note=Secreted via type III secretion system (TTSS), delivered into the
CC       host intercellular space. HrpZ travels through the hrp pilus and it is
CC       secreted only from the pilus tip (By similarity). {ECO:0000250}.
CC   -!- DOMAIN: The glycine-rich region is characteristic of harpins.
CC   -!- SIMILARITY: Belongs to the harpin HrpZ family. {ECO:0000305}.
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DR   EMBL; AF092879; AAC63363.1; -; Genomic_DNA.
DR   AlphaFoldDB; O87653; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR   GO; GO:0034053; P:modulation by symbiont of host defense-related programmed cell death; IEA:UniProtKB-KW.
DR   InterPro; IPR006961; HrpN/Z.
DR   Pfam; PF04877; Harpin; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Host cell membrane; Host membrane;
KW   Hypersensitive response elicitation; Ion channel; Ion transport; Membrane;
KW   Repeat; Secreted; Transport; Virulence.
FT   CHAIN           1..342
FT                   /note="Harpin HrpZ"
FT                   /id="PRO_0000220299"
FT   REPEAT          211..217
FT                   /note="1-1"
FT   REPEAT          266..272
FT                   /note="1-2"
FT   REPEAT          277..280
FT                   /note="2-1"
FT   REPEAT          312..315
FT                   /note="2-2"
FT   REGION          200..248
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          211..272
FT                   /note="2 X 7 AA repeats of G-G-G-L-G-T-P"
FT   REGION          267..287
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          277..315
FT                   /note="2 X 4 AA repeats of Q-T-[GA]-T"
FT   COMPBIAS        214..248
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        271..287
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   342 AA;  34669 MW;  8D08737596CADDEA CRC64;
     MQSLSLNSSS LQTPAMALVL VRPETETTGA STSSRALQEV VVKLAEELMR NGQLDDSSPL
     GKLLAKSMAA DGKAGGGIED VIAALDKLIH EKLGDNFGAS ADNASGTGQQ DLMTQVLSGL
     AKSMLDDLLT KQDGGSSFSE DDMPMLSKIA QFMDDNPAQF PKPDSGSWVN ELKEDNFLDG
     DKTAAFRSAL DIIGQQLGNQ QSDAGGLAGT GGGLGTPSSF SNNSSVTGDP LIGANTGPGD
     SGNSSSEAGQ LIGEFIDRGL QSVLAGGGLG TPVNTPQTGT AANGGQSAQD LDQLLGGLLL
     KGLEATLKDA GQTATDVQSS AAQIATLLVS TLLQGTRNQA AA