ID   HRPZ_PSESG              Reviewed;         345 AA.
AC   Q52481;
DT   26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 60.
DE   RecName: Full=Harpin HrpZ;
DE   AltName: Full=Harpin-Psg;
DE   AltName: Full=HrpZ-Psg protein;
GN   Name=hrpZ;
OS   Pseudomonas savastanoi pv. glycinea (Pseudomonas syringae pv. glycinea).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=318;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION IN HR.
RC   STRAIN=Race 4;
RX   PubMed=7579616; DOI=10.1094/mpmi-8-0717;
RA   Preston G., Huang H.-C., He S.Y., Collmer A.;
RT   "The HrpZ proteins of Pseudomonas syringae pvs. syringae, glycinea, and
RT   tomato are encoded by an operon containing Yersinia ysc homologs and elicit
RT   the hypersensitive response in tomato but not soybean.";
RL   Mol. Plant Microbe Interact. 8:717-732(1995).
CC   -!- FUNCTION: Harpins are proteins able to elicit hypersensitive response
CC       (HR) in non-host plants and are required for pathogenicity in host
CC       plants. HrpZ forms ion-conducting pores permeable for cations. Such
CC       pore-forming activity may allow nutrient release and/or delivery of
CC       virulence factors during bacterial colonization of host plants (By
CC       similarity). {ECO:0000250, ECO:0000269|PubMed:7579616}.
CC   -!- SUBUNIT: Homomultimeric. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}. Host cell membrane
CC       {ECO:0000250}. Note=Secreted via type III secretion system (TTSS),
CC       delivered into the host intercellular space. HrpZ travels through the
CC       hrp pilus and it is secreted only from the pilus tip (By similarity).
CC       {ECO:0000250}.
CC   -!- DOMAIN: The glycine-rich region is characteristic of harpins.
CC   -!- SIMILARITY: Belongs to the harpin HrpZ family. {ECO:0000305}.
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DR   EMBL; L41862; AAB00136.1; -; Genomic_DNA.
DR   RefSeq; WP_004656200.1; NZ_RBVH01000303.1.
DR   AlphaFoldDB; Q52481; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR   GO; GO:0034053; P:modulation by symbiont of host defense-related programmed cell death; IEA:UniProtKB-KW.
DR   InterPro; IPR006961; HrpN/Z.
DR   Pfam; PF04877; Harpin; 1.
PE   1: Evidence at protein level;
KW   Host cell membrane; Host membrane; Hypersensitive response elicitation;
KW   Ion channel; Ion transport; Membrane; Repeat; Secreted; Transport;
KW   Virulence.
FT   CHAIN           1..345
FT                   /note="Harpin HrpZ"
FT                   /id="PRO_0000220303"
FT   REPEAT          213..219
FT                   /note="1-1"
FT   REPEAT          269..275
FT                   /note="1-2"
FT   REGION          202..247
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          213..275
FT                   /note="2 X 7 AA repeats of G-G-G-L-G-[ST]-P"
FT   COMPBIAS        215..229
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   345 AA;  35290 MW;  B73FB324B7F28DDC CRC64;
     MQSLSLNSST LQSPSMALVL IRPETETTGP STSSRALQEV IAQLAQELTH NGQLDESSPL
     GKLLGKAMAA SGKAGGGLED IKAALDTLIH EKLGDNFGAS ADNASDTGQH DLMTQVLNGL
     AKSMLNDLLT KQDDGTRFSE DDMPMLKKIA EFMDDNPAQF PKPDSGSWVN ELKEDNFLDG
     DETAQFRSAL DIIGQQLGSQ QNAAGGLAGD SSGGGLGSPV SNTENSPGSL GDPLIDANTG
     PASNSNSNGD VGQLIGELID RGLQSVLAGG GLGTPVSTAN TALVPGGEQP NQDLGQLLGG
     LLQKGLEATL QDAGQTGTGV QSSTAQVALL LVNMLLQSTK NQAAA