HRPZ_PSESH
ID HRPZ_PSESH Reviewed; 345 AA.
AC Q9F0B0;
DT 26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 64.
DE RecName: Full=Harpin HrpZ;
DE AltName: Full=Harpin-Psph;
DE AltName: Full=HrpZ-Psph protein;
GN Name=hrpZ;
OS Pseudomonas savastanoi pv. phaseolicola (Pseudomonas syringae pv.
OS phaseolicola).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=319;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND SUBCELLULAR LOCATION.
RC STRAIN=1302A / Race 6;
RX PubMed=11134504; DOI=10.1073/pnas.98.1.289;
RA Lee J., Kluesener B., Tsiamis G., Stevens C., Neyt C., Tampakaki A.P.,
RA Panopoulos N.J., Noeller J., Weiler E.W., Cornelis G.R., Mansfield J.W.,
RA Nuernberger T.;
RT "HrpZPsph from the plant pathogen Pseudomonas syringae pv. phaseolicola
RT binds to lipid bilayers and forms an ion-conducting pore in vitro.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:289-294(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=1302A / Race 6;
RX AGRICOLA=IND43617657; DOI=10.1111/j.1364-3703.2004.00212.x;
RA Gropp S.J., Guttman D.S.;
RT "The PCR amplification and characterization of entire Pseudomonas syringae
RT hrp/hrc clusters.";
RL Mol. Plant Pathol. 5:137-140(2004).
RN [3]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RC STRAIN=NPS 3121;
RX PubMed=11106029; DOI=10.1094/mpmi.2000.13.12.1366;
RA Tampakaki A.P., Panopoulos N.J.;
RT "Elicitation of hypersensitive cell death by extracellularly targeted
RT HrpZPsph produced in planta.";
RL Mol. Plant Microbe Interact. 13:1366-1374(2000).
RN [4]
RP FUNCTION.
RX PubMed=11340183; DOI=10.2307/3871365;
RA Lee J., Klessig D.F., Nuernberger T.;
RT "A harpin binding site in tobacco plasma membranes mediates activation of
RT the pathogenesis-related gene HIN1 independent of extracellular calcium but
RT dependent on mitogen-activated protein kinase activity.";
RL Plant Cell 13:1079-1093(2001).
RN [5]
RP TRANSCRIPTIONAL REGULATION.
RC STRAIN=1449B / Race 7, and 1488A / Race 7;
RX PubMed=15553250; DOI=10.1094/mpmi.2004.17.11.1250;
RA Thwaites R., Spanu P.D., Panopoulos N.J., Stevens C., Mansfield J.W.;
RT "Transcriptional regulation of components of the type III secretion system
RT and effectors in Pseudomonas syringae pv. phaseolicola.";
RL Mol. Plant Microbe Interact. 17:1250-1258(2004).
RN [6]
RP FUNCTION.
RC STRAIN=Race 6;
RX PubMed=15672819; DOI=10.1094/mpmi-18-0060;
RA Li C.-M., Haapalainen M., Lee J., Nuernberger T., Romantschuk M., Taira S.;
RT "Harpin of Pseudomonas syringae pv. phaseolicola harbors a protein binding
RT site.";
RL Mol. Plant Microbe Interact. 18:60-66(2005).
CC -!- FUNCTION: Harpins are proteins able to elicit hypersensitive response
CC (HR) in non-host plants and are required for pathogenicity in host
CC plants. HrpZ forms ion-conducting pores permeable for cations. Such
CC pore-forming activity may allow nutrient release and/or delivery of
CC virulence factors during bacterial colonization of host plants. Also
CC binds peptides, which contain tryptophan and leucine, suggesting a
CC protein-protein interaction between the harpin and a host plant
CC protein, possibly involved in the bacterial pathogenesis.
CC {ECO:0000269|PubMed:11106029, ECO:0000269|PubMed:11340183,
CC ECO:0000269|PubMed:15672819}.
CC -!- SUBUNIT: Homomultimeric. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted. Host cell membrane {ECO:0000305}.
CC Note=Secreted via type III secretion system (TTSS), delivered into the
CC host intercellular space. HrpZ travels through the hrp pilus and it is
CC secreted only from the pilus tip (By similarity). It is found to
CC associate stably with liposomes and synthetic bilayer membranes.
CC {ECO:0000250}.
CC -!- INDUCTION: Expression enhanced by contact with host cell wall. Also
CC regulated by HrpRS and HrpL. {ECO:0000269|PubMed:15553250}.
CC -!- DOMAIN: The glycine-rich region is characteristic of harpins.
CC -!- SIMILARITY: Belongs to the harpin HrpZ family. {ECO:0000305}.
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DR EMBL; AF268940; AAF99292.1; -; Genomic_DNA.
DR EMBL; AY530203; AAS20457.1; -; Genomic_DNA.
DR RefSeq; WP_011167991.1; NZ_RBTC01000005.1.
DR AlphaFoldDB; Q9F0B0; -.
DR PATRIC; fig|319.14.peg.1560; -.
DR OMA; MDDNPAQ; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR GO; GO:0034053; P:modulation by symbiont of host defense-related programmed cell death; IEA:UniProtKB-KW.
DR InterPro; IPR006961; HrpN/Z.
DR Pfam; PF04877; Harpin; 1.
PE 2: Evidence at transcript level;
KW Host cell membrane; Host membrane; Hypersensitive response elicitation;
KW Ion channel; Ion transport; Membrane; Repeat; Secreted; Transport;
KW Virulence.
FT CHAIN 1..345
FT /note="Harpin HrpZ"
FT /id="PRO_0000220300"
FT REPEAT 213..219
FT /note="1-1"
FT REPEAT 269..275
FT /note="1-2"
FT REGION 86..194
FT /note="Peptide-binding"
FT REGION 202..247
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 213..275
FT /note="2 X 7 AA repeats of G-G-G-L-G-[ST]-P"
FT COMPBIAS 215..229
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 345 AA; 35250 MW; A4B0B23A67268CA2 CRC64;
MQSLSLNSST LQSPSMALVL IRPETETTGS STSSRALQEV IAQLAQELTH NGQLDESSPL
GKLLGKAMAA SGKAGGGLED IKAALDTLIH EKLGDNFGAS ADNASDTGQH DLMTQVLNGL
AKSMLNDLLT KQDDGTRFSE DDMPMLKKIA EFMDDNPAQF PKPDSGSWVN ELKEDNFLDG
DETAQFRSAL DIIGQQLGSQ QNAAGGLAGD SSGGGLGSPV SNTENSPGSL GDPLIDANTG
PASNSNSNGD VGQLIGELID RGLQSVLAGG GLGTPVSTAN TALVPGGEQP NQDLGQLLGG
LLQKGLEATL QDAGQTGTGV QSSAAQVALL LVNMLLQSTK NQAAA
